We narrowed to 14,579 results for: pET

1. pET30a-SRAP-delCys2

   Plasmid

   #84568

   Purpose

   Encodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2

   Depositor

   Douglas Feldman

   Article

   Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

   Insert

   Srap1 (Hmces Mouse)
   

   Use

   Tags

   6xHis and S-Tag

   Expression

   Bacterial

   Mutation

   deletion of Cys2

   Promoter

   T7

   Available since

   Nov. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a-mAIM2-deltaPYD

   Plasmid

   #51535

   Purpose

   Express His6-tagged HIN domain of mAIM2 in bacteria

   Depositor

   Emad Alnemri

   Article

   Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

   Insert

   Aim2 (Aim2 Mouse)
   

   Use

   Tags

   His6 and T7

   Expression

   Bacterial

   Mutation

   Promoter

   lac operator

   Available since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-PesaR-Clux

   Plasmid

   #47658

   Purpose

   PesaR-C promoter, luxCDABE, and terminator in pET17b(AmpR)

   Depositor

   Cynthia Collins

   Article

   Shong et al ACS Synth Biol. 2013 Jul 23.

   Insert

   PesaR-C
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Additional esa box between -35 site and the origi…

   Promoter

   PesaR-C

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

4. human TLNRD1 pET151

   Plasmid

   #159384

   Purpose

   Expresses human TLNRD1 in bacterial cells

   Depositor

   Ben Goult

   Article

   Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   Talin Rod Domain Containing Protein 1 (TLNRD1 Human)
   

   Use

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CyPet-A206K-PRL2

   Plasmid

   #149685

   Purpose

   For the FRET-based binding assay between human CNNM3 CBS domain and PRL2.

   Depositor

   Motoyuki Hattori

   Article

   Cai et al Sci Rep. 2020 Jul 30;10(1):12879. doi: 1

   Insert

   CyPet-A206K-PRL2
   

   Use

   Tags

   8 histidine tag

   Expression

   Bacterial

   Mutation

   CyPet A206K

   Promoter

   T7

   Available since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28 Set9 mutant

   Plasmid

   #24083

   Depositor

   Danny Reinberg

   Article

   Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

   Insert

   Set9 (SETD7 Human)
   

   Use

   Tags

   FLAG and His

   Expression

   Bacterial

   Mutation

   H297A

   Promoter

   Available since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pET-28a(+)-FPPS

   Plasmid

   #108952

   Purpose

   heterologous expression of FPPS of G. stearothermophilus in E. coli

   Depositor

   Frank Schulz

   Article

   Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

   Insert

   Farnesyldiphosphat synthase (FPPS)
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET21-pelB-LaG-24

   Plasmid

   #172750

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-24
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21-pelB-LaG-9

   Plasmid

   #172742

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-9
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. C122-E16: CyPet

    Plasmid

    #162896

    Purpose

    Standard Gateway entry clone encoding CyPet fluorescent protein

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    CyPet fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-HJURP-CBP

    Plasmid

    #30393

    Depositor

    Yawen Bai

    Article

    Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

    Insert

    HJURP CenpA Binding Domain (CENPA Human, Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pET His10-Src42a

    Plasmid

    #126674

    Purpose

    Expression of His10-Src42a for purification from E. coli

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Src42a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PET28-FnCas12a-EP16

    Plasmid

    #183636

    Purpose

    PET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.

    Depositor

    Yunzi Luo

    Article

    Wang et al Biotechnol Bioeng. 2019 Jun;116(6):1463-

    Insert

    FnCas12a-EP16
    

    Use

    CRISPR

    Tags

    6×His Tag

    Expression

    Bacterial

    Mutation

    N607R, K613V, N617R, K180S, K660R, D616N

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. HOXD9 (human) pET

    Plasmid

    #8570

    Depositor

    Corey Largman

    Insert

    HOXD9 (HOXD9 Human)
    

    Use

    Tags

    T7

    Expression

    Bacterial

    Mutation

    PCR human cDNA clone; makes protein by in vitro T…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. TrmD_HaeIn/pET28b(+) N,C-tag

    Plasmid

    #12665

    Depositor

    SeWon Suh

    Article

    Kim et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    tRNA (guanine-N(1)-)-methyltransferase (trmD Haemophilus influenzae)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET20b- hTPI Lys13Arg

    Plasmid

    #50726

    Purpose

    overexpression of the human TPI1 Lys13Arg allele in E.coli

    Depositor

    Markus Ralser

    Article

    Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

    Insert

    Triosephosphate isomerase 1 (TPI1 Human)
    

    Use

    Tags

    6xHIS tag

    Expression

    Bacterial

    Mutation

    changed Lysine 13 to Argenine

    Promoter

    T7

    Available since

    Feb. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-GST-Q11

    Plasmid

    #185790

    Purpose

    Encodes glutathione S-transferase linked to Q11 self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    GST-Q11
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-MBP-creM

    Plasmid

    #112238

    Purpose

    pET28-derived plasmid that expresses an N-terminal maltose binding fusion construct of CreM

    Depositor

    Emily Balskus

    Article

    Waldman et al J Org Chem. 2018 May 29. doi: 10.1021/ac

    Insert

    CreM
    

    Use

    Tags

    MBP

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28-p53 (Mouse 1-87)

    Plasmid

    #62061

    Purpose

    Expression of Mouse p53 (residues 1-87) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

    Insert

    p53 (Trp53 Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-87

    Promoter

    T7

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pET-21a(+)-IS200

    Plasmid

    #12604

    Depositor

    SeWon Suh

    Article

    Lee et al J Biol Chem. 2006 Feb 17. 281(7):4261-6.

    Insert

    IS200 transposase (SSO1474 Sulfolobus solfataricus)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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