We narrowed to 14,886 results for: pET

1. pET11a-dH3-dH4

   Plasmid

   #105502

   Purpose

   Co-expression of Drosophila histones H3 and H4

   Depositor

   James Kadonaga

   Article

   Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

   Insert

   dH3-dH4
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 5787 pET28aSX104B/ pES42

   Plasmid

   #1229

   Depositor

   Susan Lindquist

   Article

   Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

   Insert

   Hsp104 (HSP104 Budding Yeast)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

3. pET28_6xHis-PARP1-Y986H

   Plasmid

   #174801

   Purpose

   Bacterial expression of a PARP1 mutant that produces highly branched but short PAR chains overall

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-Y986H (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Y986H, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28_6xHis-PARP1-Y986S

   Plasmid

   #174802

   Purpose

   Bacterial expression of a PARP1 mutant that produces mainly short PAR oligomers

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-Y986S (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Y986H, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28_6xHis-PARP1-G972R

   Plasmid

   #174800

   Purpose

   Bacterial expression of a PARP1 mutant that produces less PAR oligomers overall

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-G972R (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   G972R, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-pro-sumo3

   Plasmid

   #25103

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   pro-SUMO3 (SUMO3 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   changed Serine 2 to Alanine

   Promoter

   Available since

   June 22, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pETa(+) HpCsd3 (42-403)

   Plasmid

   #71285

   Purpose

   Expresses Csd3 from Helicobacter pylori

   Depositor

   SeWon Suh

   Article

   An et al Acta Crystallogr D Biol Crystallogr. 201

   Insert

   HP0506 (HP0506 )
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pFS_0143-pET30-Fusicatenibacter_saccharivorans

   Plasmid

   #116952

   Purpose

   Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with classic acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Fusicatenibacter saccharivorans RT-Cas1-Cas2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET14b PHAS-I F113A

   Plasmid

   #15680

   Depositor

   John Lawrence

   Article

   Choi et al J Biol Chem. 2003 May 30. 278(22):19667-

   Insert

   PHAS-I F113A (Eif4ebp1 Rat)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F113A mutation.

   Promoter

   Available since

   Sept. 24, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pET22b-T5-FB-E25TAG-F6C

    Plasmid

    #176546

    Purpose

    Expresses IgG affinity protein FB(F6C) with a TAG stop codon at E25 position

    Depositor

    Han Xiao

    Article

    Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

    Insert

    IgG affinity protein
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    change phenylalanine 6 to cysteine; change glutam…

    Promoter

    T5

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-PLS-KP6_rpoA-C

    Plasmid

    #190966

    Purpose

    Test the C-to-U editing activity of KP6 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET51b-NAD-cpLuc

    Plasmid

    #135628

    Purpose

    Bacterial expression of a biosensor for measuring NAD+ levels at the point of care (V1)

    Depositor

    Kai Johnsson

    Article

    Yu et al Nat Metab. 2019 Dec;1(12):1219-1225. doi

    Insert

    NAD-cpLuc sensor
    

    Use

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-Hisx6-Sirt6

    Plasmid

    #20277

    Depositor

    Leonard Guarente

    Article

    Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

    Insert

    Sirt6 (Sirt6 Mouse)
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pET SUMO-FL-syt1

    Plasmid

    #213628

    Purpose

    Bacterial expression of SUMO-tagged rat FL-syt1

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    FL-syt1 (Syt1 Rat)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET19-M(BRCT)TdTwt

    Plasmid

    #207459

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domain

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    M(BRCT)TdTwt (Dntt Mouse)
    

    Use

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-ChE1 v4.5

    Plasmid

    #185755

    Purpose

    Bacterial expression of ChE1 v4.5

    Depositor

    Jeffrey Bode

    Article

    Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

    Insert

    ChE1 v4.5
    

    Use

    Tags

    His10

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETM-60-NusA-DmBelle_B

    Plasmid

    #145963

    Purpose

    Bacterial Expression of DmBelle

    Depositor

    Elisa Izaurralde

    Insert

    DmBelle (bel Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    del 155-157, T530A and I685V

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-DUET-Prp18

    Plasmid

    #111354

    Purpose

    protein purification

    Depositor

    Christine Guthrie

    Insert

    PRP18
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET30-hDlg PDZ2

    Plasmid

    #21692

    Depositor

    James Baleja

    Article

    Liu et al Biochemistry. 2007 Sep 25. 46(38):10864-

    Insert

    hDlg/SAP97 PDZ2 domain (DLG1 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    human hDlg/SAP97 PDZ2 domain (residues 318-405)

    Promoter

    Available since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pET16b-Trx C35S

    Plasmid

    #36947

    Depositor

    Anna Blom

    Article

    King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

    Insert

    Thioredoxin-1 C35S mutation (TXN Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    cys 35 to serine

    Promoter

    T7

    Available since

    May 20, 2013

    Availability

    Academic Institutions and Nonprofits only