We narrowed to 2,318 results for: pET28

1. Fast-GCaMP-EF16

   Plasmid

   #52642

   Purpose

   genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

   Depositor

   Samuel Wang

   Article

   Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

   Insert

   Fast-GCaMP-EF16
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D431N/D433N/D435N/E442A mutated from GCaMP3

   Promoter

   T7

   Available since

   April 23, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pJBL717

   Plasmid

   #140387

   Purpose

   Expresses MobR protein under the control of T7-lacO promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-lacO-MobR-T
   

   Use

   Tags

   TEV-6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Fast-GCaMP-EF19

   Plasmid

   #52644

   Purpose

   genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

   Depositor

   Samuel Wang

   Article

   Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

   Insert

   Fast-GCaMP-EF19
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T328D/T330D mutated from GCaMP3

   Promoter

   T7

   Available since

   April 23, 2014

   Availability

   Academic Institutions and Nonprofits only

4. Human USP30 (64-502, construct 13, MG-26-82)

   Plasmid

   #110746

   Purpose

   Bacterial expression of human USP30 (construct 13)

   Depositor

   David Komander

   Article

   Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

   Insert

   USP30 (USP30 Human)
   

   Use

   Tags

   N-His6-3C-GST-3C

   Expression

   Bacterial

   Mutation

   F348D, M350S, I353E + insertion deletion: 64-178;…

   Promoter

   T7

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pStrepIHFb

   Plasmid

   #149385

   Purpose

   Expression of P. aeruginosa PA14, StrepII-HRV3C-IHFbeta

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 StrepII-tagged IHFbeta
   

   Use

   Tags

   HRV3C cleavage site and StrepII-tag with

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SENP2M497A

   Plasmid

   #61088

   Purpose

   cleave the SUMO off when use PETSUMO vector for express fusion protein

   Depositor

   Cynthia Wolberger

   Article

   Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

   Insert

   SENP2M497A catalytic domain (SENP2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   M497A; Catalytic domain is residues 364-589

   Promoter

   T7

   Available since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pET-28a-GFP-Patronin CC 868–1457

   Plasmid

   #59053

   Purpose

   for E. coli expression of Patronin CC truncation

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   Patronin (Patronin Fly)
   

   Use

   Tags

   His, Strep, TEV, and sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

8. HOXC13(human)-pET

   Plasmid

   #8679

   Depositor

   Corey Largman

   Article

   Komuves et al Dev Dyn 2003 Jun;227(2):192-202.

   Insert

   HOXC13 (HOXC13 Human)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   makes protein by Coomassie blue staining

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pET-28a(+)-His(6)-TEV-AtPCO4-1(wt)

   Plasmid

   #191801

   Purpose

   Full length wt AtPCO4 with Nt-His tag

   Depositor

   Emily Flashman

   Article

   White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

   Insert

   AtPCO4-1
   

   Use

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Pediculus humanus corporis PINK1 (143 - 575)

    Plasmid

    #110751

    Purpose

    Bacterial expression of PhPINK1 (crystallography)

    Depositor

    David Komander

    Article

    Schubert et al Nature. 2017 Dec 7;552(7683):51-56. doi:

    Insert

    Phum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)
    

    Use

    Tags

    N-His6-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HOXA9 (murine) HIS-tag pET

    Plasmid

    #8492

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HoxA9 (Hoxa9 Mouse)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    BamHI/BalI fusion into amino acid #2 of HOXA9; ma…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

12. Fast-GCaMP-RS15

    Plasmid

    #52652

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-RS15
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted G47/H48 from GCaMP3

    Promoter

    T7

    Available since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

13. MALT1-DD (2G7R)

    Plasmid

    #25613

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    MALT1-DD (MALT1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains Death Domain

    Promoter

    Available since

    Aug. 31, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

14. 4E2H.210_LUMABS

    Plasmid

    #155209

    Purpose

    Expresses 4E2H.210_LUMABS sensor in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    4E2H.210_LUMABS
    

    Use

    Tags

    6x His Tag

    Expression

    Mutation

    Promoter

    Available since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Dom34+GST

    Plasmid

    #19099

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Use

    Tags

    C tag, GST, and N tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

16. Tip alpha (21-192)

    Plasmid

    #23157

    Depositor

    SeWon Suh

    Article

    Jang et al J Mol Biol. 2009 Sep 11. 392(1):191-7.

    Insert

    TNF-α-inducing protein
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-20

    Promoter

    Available since

    April 16, 2010

    Availability

    Academic Institutions and Nonprofits only

17. Fast-GCaMP-RS06

    Plasmid

    #52647

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-RS06
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M374Q mutated from GCaMP3

    Promoter

    T7

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

18. Fast-GCaMP-RS14

    Plasmid

    #52651

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-RS14
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted G47 from GCaMP3

    Promoter

    T7

    Available since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

19. Tip alpha (28-192)

    Plasmid

    #23158

    Depositor

    SeWon Suh

    Article

    Jang et al J Mol Biol. 2009 Sep 11. 392(1):191-7.

    Insert

    TNF-α-inducing protein
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-27

    Promoter

    Available since

    April 16, 2010

    Availability

    Academic Institutions and Nonprofits only

20. 3JZN

    Plasmid

    #60123

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    EED:APC056-C06:C32050 (EED EED:APC056-C06:C32050, Human)
    

    Use

    Tags

    6xHis and GST

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits