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pHH0103_SHANK1-1/1

Plasmid

#91517

Purpose

Protein expression and purification of human SH3 domain construct SHANK1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SHANK1-1/1 (SHANK1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFA3

Plasmid

#46790

Purpose

To express ATPase inactive Fun30 in budding yeast

Depositor

Patrick Varga-Weisz

Article

Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

Insert

FUN30 (FUN30 Budding Yeast)

Tags

V5

Expression

Yeast

Mutation

ATP-binding site mutated replaced lysin 603 by ar…

Promoter

PGAL1

Available Since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only
CMV-CC2

Plasmid

#51221

Purpose

expression of amino acid 917-1040 of chicken DCTN1 p150Glued in mammalian cells

Depositor

Trina Schroer

Article

Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

Insert

DCTN1 (DCTN1 Chicken)

Expression

Mammalian

Mutation

aa917-1040

Promoter

CMV

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only
fgfprom luc

Plasmid

#69446

Purpose

basal plasmid containing minimal fgf4 promoter used for testing enhancer activity of DNAs inserted immediately upstream of the promoter.

Depositor

Lisa Dailey

Article

Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

Insert

minimal fgf4 promoter

Use

Luciferase

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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PX458_GATAD2A_2

Plasmid

#86334

Purpose

Encodes gRNA for 3' target of human GATAD2A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against GATAD2A (GATAD2A Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_GATAD2A_1

Plasmid

#86333

Purpose

Encodes gRNA for 3' target of human GATAD2A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against GATAD2A (GATAD2A Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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CaM-RhoA(DP)-Venus

Plasmid

#31994

Depositor

Kevin Truong

Article

Mills et al Cell Calcium. 2010 Oct;48(4):195-201.

Insert

CaM-RhoA(DP) (RHOA Human)

Tags

YFP

Expression

Bacterial and Mammalian

Mutation

Q63L mutation in RhoA, E7S and D65Y in CaM

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only
pPK1E-2+t

Plasmid

#48148

Purpose

Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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SMT205

Plasmid

#134817

Purpose

SMT201 with mutated RBS for DHFR, in vivo assays

Depositor

Tanja Kortemme

Article

Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

Inserts

Dihydrofolate reductase
Thymidylate synthase

Expression

Bacterial

Mutation

Silent mutations to eliminate internal restrictio…

Promoter

T7 and TET

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCNL-N1_ITPKA

Plasmid

#65688

Purpose

Expresses ITPKA-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

inositol triphosphate 3-kinase A

Use

Luciferase

Tags

CNL

Expression

Mammalian

Mutation

contains residues 9-52

Promoter

CMV

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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