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Showing: 5351 - 5400 of 13339 results

  19. pEM9_CL7_Bac_2NLS-iCas12a (LbCas12a)-4NLS

    Plasmid
    #244844
    Purpose
    Bacterial expression of iCas12a LbCas12a with 2xSV40 nuclear localization sequence for protein purification
    Depositor
    Jennifer Doudna
    Article
    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka
    Insert
    iCas12a
    Use
    CRISPR
    Tags
    10xHis-CL7-2xSV40 NLS and 4xSV40 NLS
    Expression
    Bacterial
    Mutation
    F863V/D952N/C965Y/V1011A/Q1108L/A1113V/S1132T/S12…
    Promoter
    T7
    Available Since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    244844-plasmid-map-sequence-id-486520

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  25. pRECMYC-Eco2

    Plasmid
    #240706
    Purpose
    pLAM12 vector backbone containing Eco2 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Eco2 RT, Eco1 ncRNA, MspRecT
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240706-plasmid-map-sequence-id-483558

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  27. pREC1010-Kva1

    Plasmid
    #240684
    Purpose
    RSF1010 origin of replication plasmid containing Kva1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Kva1 RT,Kva1 ncRNA, Beta and EcSSB
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240684-plasmid-map-sequence-id-483595

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  34. pUC19_21 tRNA genes

    Plasmid
    #242430
    Purpose
    In vitro transcription of 21 tRNAs after Nt.BspQI treatment. The plasmid contains a T7 promoter upstream and an Nt.BspQI recognition site downstream of each tRNA gene.
    Depositor
    Sebastian Maerkl
    Article
    Li et al Nat Commun. 2025 Jul 5;16(1):6212. doi:
    Insert
    21 tRNA genes with a T7 promoter upstream and an Nt.BspQI recognition site downstream of each tRNA gene
    Expression
    Bacterial
    Available Since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    242430-plasmid-map-sequence-id-485004

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  39. pNS1D-sgRNA9-SpR

    Plasmid
    #191092
    Purpose
    aTc inducible gRNA expression plasmid with BbsI sites for Golden Gate insertion of crRNA oligos and homology arms for genome integration at NS1D in Synechococcus sp. PCC 7002
    Depositor
    Anne Ruffing
    Article
    Dallo et al ACS Synth Biol. 2023 Apr 21;12(4):1175-1
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    tet
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    191092-plasmid-map-sequence-id-373360

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  40. pRECMYC-Tsp1

    Plasmid
    #240712
    Purpose
    pLAM12 vector backbone containing Tsp1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Tsp11 RT, Eco1 ncRNA, MspRecT
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240712-plasmid-map-sequence-id-482753

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  41. pRECMYC-Kva1

    Plasmid
    #240709
    Purpose
    pLAM12 vector backbone containing Kva1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Kva1 RT, Eco1 ncRNA, MspRecT
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240709-plasmid-map-sequence-id-482751

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  42. pRECMYC-Hcl1

    Plasmid
    #240714
    Purpose
    pLAM12 vector backbone containing Hcl1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Hcl1 RT, Eco1 ncRNA, MspRecT
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240714-plasmid-map-sequence-id-482749

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  43. pRECMYC-Eas1

    Plasmid
    #240710
    Purpose
    pLAM12 vector backbone containing Eas1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Eas1 RT, Eco1 ncRNA, MspRecT
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    240710-plasmid-map-sequence-id-482711

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  48. pRECMYC-Csp1

    Plasmid
    #240711
    Purpose
    pLAM12 vector backbone containing Csp1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Csp1 RT, Eco1 ncRNA, MspRecT
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    240711-plasmid-map-sequence-id-482471

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  49. pRECMYC-Abe1

    Plasmid
    #240708
    Purpose
    pLAM12 vector backbone containing Abe1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Abe1 RT, Eco1 ncRNA, MspRecT
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    240708-plasmid-map-sequence-id-482469

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  50. pRECMYC-Ban1

    Plasmid
    #240707
    Purpose
    pLAM12 vector backbone containing Ban1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Ban1 RT, Eco1 ncRNA, MspRecT
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    240707-plasmid-map-sequence-id-482428

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Showing: 5351 - 5400 of 13339 results