We narrowed to 165,385 results for: Gene

1. pLVX_TRE3G_DD::mCherry

   Plasmid

   #108679

   Purpose

   Tunable system for controlled gene expression and protein stability in mammalian cells

   Depositor

   Lucia Marucci

   Article

   Pedone et al Nat Commun. 2019 Oct 2;10(1):4481. doi:

   Insert

   Destabilizing Domain (DD) mCherry fusion protein
   

   Use

   Lentiviral

   Tags

   DD::mCherry fusion protein

   Expression

   Mammalian

   Promoter

   Doxycycline inducible

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu

   Plasmid

   #216421

   Purpose

   Empty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.

   Depositor

   Ellen Bushell

   Article

   Jonsdottir et al bioRxiv 2024.04.20.590404

   Type

   Empty backbone

   Use

   CRISPR; Expression in plasmodium berghei

   Tags

   3xmyc

   Expression

   Bacterial

   Available Since

   April 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPbU6_hdhfr/yfcu

   Plasmid

   #216422

   Purpose

   Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter for traditional CRISPR editing.

   Depositor

   Ellen Bushell

   Article

   Jonsdottir et al bioRxiv 2024.04.20.590404

   Type

   Empty backbone

   Use

   CRISPR; Expression in plasmodium berghei

   Expression

   Bacterial

   Available Since

   April 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMC_mNG2(11)_BSDminus_F2

   Plasmid

   #184164

   Purpose

   Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Inserts

   mNG2(11)
   bsd
   

   Use

   CRISPR

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAluYa5-neo-TET

   Plasmid

   #51283

   Purpose

   expresses Alu, a human SINE, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette

   Depositor

   Astrid Roy-Engel

   Article

   Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

   Insert

   AluYa5
   

   Tags

   neoTET cassette with a tetrahymena self-splicing …

   Expression

   Mammalian

   Promoter

   upstream pol III enhancer sequence of the 7SL gen…

   Available Since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. M249-5'csr-1-Pgsy-1-luciferase-Bar-3'csr-1

   Plasmid

   #89693

   Purpose

   Luciferase reporter plasmid targeted at csr-1 locus

   Depositor

   Christian Hong

   Article

   Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

   Insert

   gsy-1 promoter, luciferase
   

   Use

   Yeast episomal vector

   Tags

   luciferase

   Promoter

   gsy-1

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG Cas9-2A-Citrine

   Plasmid

   #92358

   Purpose

   CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   Cas9 2A Citrine
   

   Expression

   Mammalian

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUDP004

   Plasmid

   #101165

   Purpose

   E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   ScTDH3

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Htr5b(3.7)-tTA-sv40

   Plasmid

   #184412

   Purpose

   AAV expression of tTA from mouse 5-hydroxytryptamine (serotonin) receptor 5B (Htr5b) gene promoter.

   Depositor

   Marylka Yoe Uusisaari

   Article

   Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

   Insert

   Mouse Htr5b gene promoter-tTA
   

   Use

   AAV

   Tags

   tTA

   Expression

   Mammalian

   Promoter

   Mouse Htr5b gene

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMDC32B-AtMIR390a-B/c

    Plasmid

    #51776

    Purpose

    Plant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor

    Depositor

    James Carrington

    Article

    Carbonell et al Plant Physiol. 2014 Mar 19.

    Insert

    AtMIR390a-B/c
    

    Use

    Plant expression

    Mutation

    A. thaliana MIR390a precursor sequence including …

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMaCTag-P29

    Plasmid

    #120040

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Strep-II

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Strep-II
    

    Use

    Pcr template

    Tags

    Strep-II

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRvTUB-mEGFP-NLS

    Plasmid

    #205011

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the tubulin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP-NLS flanked by 1kbp upstream and downstream of the tubulin gene from R. varieornatus
    

    Use

    Tardigrade expression

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR-AtMIR390a-B/c

    Plasmid

    #51778

    Purpose

    Gateway-compatible entry vector for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor

    Depositor

    James Carrington

    Article

    Carbonell et al Plant Physiol. 2014 Mar 19.

    Insert

    AtMIR390a-B/c
    

    Use

    Gateway entry vector

    Mutation

    A. thaliana MIR390a precursor sequence including …

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPEPY-PF6-lacI

    Plasmid

    #85589

    Purpose

    Work as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniae

    Depositor

    Jan-Willem Veening

    Article

    Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

    Insert

    lacI
    

    Expression

    Bacterial

    Promoter

    PF6

    Available Since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. SPDK3860

    Plasmid

    #199245

    Purpose

    Modified Tobacco rattle virus (TRV) RNA2 vector for editing of Nicotiana benthamiana Phytoene desaturase (PDS) gene

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
    

    Use

    T-dna vector

    Promoter

    pPEBV

    Available Since

    June 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRvSAHS1-mEGFP-NLS

    Plasmid

    #205005

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
    

    Use

    Tardigrade expression

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRvCAHS3-mEGFP-NLS

    Plasmid

    #205004

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP-NLS flanked by 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
    

    Use

    Tardigrade expression

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Equalizer-L plasmid

    Plasmid

    #169732

    Purpose

    Equalizer variant plasmid with low eGFP output levels but the best gene dosage compensation capability.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    pCMV-tetO2

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. BC1450-H2B 5'HA-mCherry-CRISPR-Tag_V2-3'HA (HDR donor)

    Plasmid

    #164041

    Purpose

    CRISPR donor plasmid to insert mCherry-CRISPR-TagV2 into the C-terminus of human H2B gene

    Depositor

    Baohui Chen

    Article

    Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

    Insert

    mCherry-CRISPR-TagV2
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAP01

    Plasmid

    #59981

    Purpose

    The function of this material is to facilitate the capture and expression of bacterial gene clusters.

    Depositor

    Bradley Moore

    Article

    Yamanaka et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

    Type

    Empty backbone

    Use

    Synthetic Biology; Transformation-associated reco…

    Tags

    no

    Expression

    Bacterial and Yeast

    Promoter

    no

    Available Since

    Nov. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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