We narrowed to 2,540 results for: organization Addgene

1. pLenti-xBE4Gam-P2A-Puro

   Plasmid

   #112676

   Purpose

   Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)-BE4Gam
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. TP63-T2A-Rox-EF1a-Rox-Venus-NLS repair template

   Plasmid

   #167971

   Purpose

   Tp63 reporter targeting repair template

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   Tp63 (TP63 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. PEX-mCherry-CRY2

   Plasmid

   #102250

   Purpose

   Expresses fusion of CRY2PHR with mCherry and PEX3 (1-42)

   Depositor

   Bianxiao Cui

   Article

   Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

   Insert

   PEX-mCherry-CRY2 (PEX3, CRY2 Human, Mustard Weed)
   

   Tags

   6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. TVBB C-term-mTurquoise2-Hygro

   Plasmid

   #169225

   Purpose

   Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Hygro at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mTurquoise2-P2A-Hygro
   

   Use

   Targeting vector backbone

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. TVBB C-term-mTurquoise2-Puro

   Plasmid

   #169224

   Purpose

   Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Puro at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mTurquoise2-P2A-Puro
   

   Use

   Targeting vector backbone

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-xBE3-P2A-Puro

   Plasmid

   #110870

   Purpose

   Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)-BE3
   

   Use

   Lentiviral

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. SOX2 KO (T2A-H2B-EGFP)

   Plasmid

   #167969

   Purpose

   SOX2 CDS replacement with T2A-H2B-EGFP

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   SRY-Box Transcription Factor 2 (SOX2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   H2B-EGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT2K-CAGGS-mouse Gfi1-IRES-GFP

   Plasmid

   #91893

   Purpose

   Mammalian expression of mouse Gfi1

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   Gfi1 (Gfi1 Mouse)
   

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pPB CAG Gfi1:FLAG-IRES-EGFP

   Plasmid

   #153945

   Purpose

   piggyBac transposon vector with CAG promoter expressing FLAG-tagged Gfi1 and EGFP

   Depositor

   Luca Tiberi

   Article

   Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

   Insert

   Gfi1 (Gfi1 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB CAG Cdk6 R31C-IRES-EGFP

    Plasmid

    #153946

    Purpose

    piggyBac transposon vector with CAG promoter expressing Cdk6 (R31C mutant) and EGFP

    Depositor

    Luca Tiberi

    Article

    Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

    Insert

    Cdk6 (Cdk6 Mouse)
    

    Expression

    Mammalian

    Mutation

    R31C

    Promoter

    CAG

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP N1 mitoAURKA

    Plasmid

    #157754

    Purpose

    Expression of AURKA:GFP targeted to mitochondria

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    AURKA without the first 30 amino acids replaced b…

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV ShadowG-AURKA-mTurq2

    Plasmid

    #157768

    Purpose

    Expression of AuroraA kinase biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    mTurquoise2 and shadowG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. TVBB C-term-Dendra2-Puro

    Plasmid

    #169217

    Purpose

    Targeting vector backbone to support a knock-in of Linker-Dendra2-P2A-Puro at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked Dendra2-P2A-Puro
    

    Use

    Targeting vector backbone

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-VQRRA-P2A-GFP-PGK-Puro

    Plasmid

    #110864

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQRRA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQRRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    D1135V, R1335Q, T1337R and NLS sequence at the N-…

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-VRERRA-P2A-GFP-PGK-Puro

    Plasmid

    #110865

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRERRA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRERRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    D1135V, G1218R, R1335E, T1337R, and NLS sequence …

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-tetON-NKX2-1-R162del-EF1a-TagRFP-2A-tet3G

    Plasmid

    #204191

    Purpose

    Doxycycline inducible lentiviral vector for human NKX2-1 (R162del) overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Deletion of R162

    Promoter

    TetON

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-tetON-NKX2-1-I207M -EF1a-TagRFP-2A-tet3G

    Plasmid

    #204213

    Purpose

    Doxycycline inducible lentiviral vector for human NKX2-1 (I207M) overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Point mutation on I207M

    Promoter

    TetON

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-tetON-NKX2-1-Q175∗-EF1a-TagRFP-2A-tet3G

    Plasmid

    #204211

    Purpose

    Doxycycline inducible lentiviral vector for human NKX2-1 (Q175∗) overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Point mutation on Q175* (*termination)

    Promoter

    TetON

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-tetON-NKX2-1-R178∗-EF1a-TagRFP-2A-tet3G

    Plasmid

    #204210

    Purpose

    Doxycycline inducible lentiviral vector for human NKX2-1 (R178∗) overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Point mutation on R178* (*termination)

    Promoter

    TetON

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-tetON-NKX2-1-I207F -EF1a-TagRFP-2A-tet3G

    Plasmid

    #204212

    Purpose

    Doxycycline inducible lentiviral vector for human NKX2-1 (I207F) overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    NK2 homeobox 1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    Point mutation on I207F

    Expression

    Mammalian

    Promoter

    TetON

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge