We narrowed to 12,677 results for: sequence

1. NUPL2_ZNF_pGEX

   Plasmid

   #135135

   Purpose

   Recombinant expression of NUPL2 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   NUPL2 (NUPL2 Human)
   

   Use

   Tags

   GST-SBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT5T_hp53_ST

   Plasmid

   #34922

   Depositor

   Thanos Halazonetis

   Article

   Petty et al EMBO J. 2011 Jun 1;30(11):2167-76. Epub

   Insert

   p53 DNA-binding (Res 1-393) (TP53 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …

   Promoter

   T7

   Available since

   Feb. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDN-D2irC6kwh

   Plasmid

   #44725

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

   Inserts

   pCMV-D2i promoter
   mCherry
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Tags

   Rabbit β-globin intron II and WPRE

   Expression

   Mammalian

   Mutation

   Initiator motif (Inr) displaced relative to pCMV-…

   Promoter

   pCMV-D2i

   Available since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-Flag-dSpCas9-HF1

   Plasmid

   #92115

   Purpose

   Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9-HF1 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, N497A, R661A, Q695A, H840A, Q926A

   Promoter

   Cbh

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. GST-AR-1-566

   Plasmid

   #104195

   Purpose

   Expresses human AR-1-566 from bacterial expression vector pGEX-5X-1 as GST fusion

   Depositor

   Elizabeth Wilson

   Article

   He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

   Insert

   human androgen receptor aa 1-566 (AR Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. EF1a_KLF7_P2A_Hygro_Barcode

   Plasmid

   #120493

   Purpose

   Barcoded lentiviral vector to express KLF7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   KLF7 (KLF7 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. RBM23_2xRRM_pGEX

   Plasmid

   #135118

   Purpose

   Recombinant expression of RBM23 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   RBM23 (RBM23 Human)
   

   Use

   Tags

   GST-SBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAGGS-Flex/3'USS(Luc)-GFP

   Plasmid

   #197887

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   CAG

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. F11-R pEBio

   Plasmid

   #61486

   Purpose

   Expresses full-length F11R (JAM1) ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag and biotinylation sequence

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   F11R (F11R Human)
   

   Use

   Tags

   enzymatic biotinylation sequence and rat CD4 d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPLN-hFluc-WT-HA-GFP11-KDEL

    Plasmid

    #177722

    Purpose

    Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    hFluc
    

    Use

    Tags

    GFP11, HA, KDEL, and Prolactin Signal Sequence

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-CAG-dDIO-lck-smMyc-4x6T

    Plasmid

    #196421

    Purpose

    Dre-dependent AAV expression of membrane-targeted Myc spaghetti monster reporter preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    Lck-smMyc
    

    Use

    AAV; Dre/rox; astrocyte-selective

    Tags

    Lck

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSP1-bio

    Plasmid

    #68505

    Purpose

    Expresses full-length P. vivax MSP1 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

    Depositor

    Julian Rayner, Gavin Wright

    Article

    Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

    Insert

    MSP1
    

    Use

    Tags

    rat CD4 d3+4, enzymatic biotinylation sequence

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Sept. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. 139H2_LC

    Plasmid

    #206202

    Purpose

    For recombinant expression of the light chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells.

    Depositor

    Joost Snijder

    Article

    Peng et al Life Sci Alliance. 2024 Mar 20;7(6):e202

    Insert

    anti-MUC1 antibody 139H2 light chain (Igkc Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. MTRAP-bio

    Plasmid

    #68530

    Purpose

    Expresses full-length P. vivax MTRAP ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

    Depositor

    Julian Rayner, Gavin Wright

    Article

    Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

    Insert

    MTRAP
    

    Use

    Tags

    rat CD4 d3+4, enzymatic biotinylation sequence

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. PVX_110945-bio

    Plasmid

    #68538

    Purpose

    Expresses full-length P. vivax PVX_110945 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

    Depositor

    Julian Rayner, Gavin Wright

    Article

    Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

    Insert

    PVX_110945
    

    Use

    Tags

    rat CD4 d3+4, enzymatic biotinylation sequence

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. VIPR1

    Plasmid

    #51865

    Purpose

    Expresses full-length Vasoactive intestinal polypeptide receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    VIPR1 (VIPR1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. TMED9-bio-His

    Plasmid

    #51855

    Purpose

    Expresses full-length Transmembrane emp24 domain-containing protein 9 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TMED9 (TMED9 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF1a_ASCL3_P2A_Hygro_Barcode

    Plasmid

    #120428

    Purpose

    Barcoded lentiviral vector to express ASCL3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    ASCL3 (ASCL3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. RIPR-bio

    Plasmid

    #68526

    Purpose

    Expresses full-length P. vivax RIPR ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

    Depositor

    Julian Rayner, Gavin Wright

    Article

    Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

    Insert

    RIPR
    

    Use

    Tags

    rat CD4 d3+4, enzymatic biotinylation sequence

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TTMP-His-bio

    Plasmid

    #52335

    Purpose

    Expresses full-length TPA-induced transmembrane protein ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TTMP (C3orf52 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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