We narrowed to 13,309 results for: sequence

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pT7-EGFP-C1-HsNot1iso1_1318-1605-IKGIRQtoDSYDAA_W

Plasmid

#147894

Purpose

Mammalian Expression of HsNot1iso1_1318-1605-IKGIRQtoDSYDAA

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_1318-1605-IKGIRQtoDSYDAA (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_YTHDF2_KO_Neo

Plasmid

#186672

Purpose

Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. Neomycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

YTHDF2 KO Neomycin (YTHDF2 Human)

Expression

Mammalian

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_ANKRD1_KO_Hygro

Plasmid

#186668

Purpose

Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, hygromycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

ANKRD1 KO Hygromycin (ANKRD1 Human)

Expression

Mammalian

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Cas9_ANKRD1_sgRNA2

Plasmid

#186669

Purpose

Plasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

ANKRD1 KO sgRNA Plasmid (ANKRD1 Human)

Expression

Mammalian

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4 TAPBPR-TM-TN6

Plasmid

#178650

Purpose

Mammalian expression of FLAG-tagged TAPBPR with MHC TM (E205K, R207E, Q209S, Q272S)

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

TAPBPR (TAPBPL Human)

Tags

FLAG and Signal/leader sequence from HLA class I …

Expression

Mammalian

Mutation

E205K, R207E, Q209S, Q272S, switched transmembran…

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-hFAM3B-HA

Plasmid

#180145

Purpose

Coding sequence of human FAM3B pCS2 with C terminal HA tag

Depositor

Edward De Robertis

Article

Zhang et al Proc Natl Acad Sci U S A. 2021 May 18;11

Insert

FAM3B (FAM3B Human)

Tags

HA

Expression

Mammalian

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-HsSyx3

Plasmid

#179289

Purpose

E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + GFP + TEV protease cleavage site

Depositor

Debra Hansen

Article

Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

Insert

Syx

Tags

His6 + GFP + TEV protease cleavage site

Expression

Bacterial

Mutation

residues 393-792 from accession number NP_0010361…

Promoter

T7

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pHsSyx3-MBP

Plasmid

#179286

Purpose

E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with C-terminal TEV protease cleavage site + mature maltose binding protein + His6

Depositor

Debra Hansen

Article

Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

Insert

Syx

Tags

TEV protease cleavage site + mature maltose bindi…

Expression

Bacterial

Mutation

residues 393-792 from accession number NP_0010361…

Promoter

T7

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-hFAM3B-FLAG

Plasmid

#180146

Purpose

Coding sequence of human FAM3B in pCS2 with C terminal FLAG tag

Depositor

Edward De Robertis

Article

Zhang et al Proc Natl Acad Sci U S A. 2021 May 18;11

Insert

FAM3B (FAM3B Human)

Tags

FLAG

Expression

Mammalian

Promoter

SP6

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot1iso1_840-1605-del1097-1110-RNFtoAAA_X

Plasmid

#148000

Purpose

Mammalian Expression of HsNot1iso1_840-1605-del1097-1110-RNFtoAAA

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_840-1605-del1097-1110-RNFtoAAA (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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