We narrowed to 2,816 results for: cdna expression plasmid
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Plasmid#218230PurposeGasdermin-D_1-275 is a pore-forming GSDMD formed by cleavage by host caspases. Transfection in HEK293 cells showed GSDMD 1-275 pores secrete 3CLpro, nucleocapsid; in excess, kills cells by pyroptosisDepositorInsertGSDMD (GSDMD Human)
TagsflagExpressionMammalianMutationconstruct only codes for amino acids 1-275Available SinceJuly 1, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-GSDMD_29-484
Plasmid#218231PurposeGasdermin-D_29-484 is a non-functional GSDMD formed by SARS-CoV-2 3CLpro cleavage in the N-terminal domain. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosisDepositorInsertGSDMD (GSDMD Human)
TagsflagExpressionMammalianMutationconstruct only codes for amino acids 29-484Available SinceJuly 1, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-GSDMD_29-270
Plasmid#218232PurposeGasdermin-D_29-270 is a non-functional GSDMD formed by two SARS-CoV-2 3CLpro cleavages. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosisDepositorInsertGSDMD (GSDMD Human)
TagsflagExpressionMammalianMutationconstruct only codes for amino acids 29-270Available SinceJuly 1, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5/FRT/TO-GAlpha12-RLuc8
Plasmid#140985PurposeEncodes a G alpha subunit (GNA12) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlpha12-RLuc8 (GNA12 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphasL-RLuc8
Plasmid#140981PurposeEncodes a G alpha subunit (GNAS1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphasL-RLuc8 (GNAS Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphaoA-RLuc8
Plasmid#140976PurposeEncodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphaoA-RLuc8 (GNAO1 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphaoB-RLuc8
Plasmid#140977PurposeEncodes a G alpha subunit (GNAO1 Isoform Alpha 2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphaoB-Rluc8 (GNAO1 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/(CAA)26 5′ leader GUG-nLuc-3XFLAG
Plasmid#127325PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (CAA)26 repeats in 5' UTRDepositorInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/(CAA)26 5′ leader AUG-nLuc-3XFLAG
Plasmid#127324PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (CAA)26 repeats in 5' UTRDepositorInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
(112) pcDNA3.1-GFP-Flag-Nup62-EPEA
Plasmid#168101PurposeGFP and Flag tagged Nup62 with EPEA nanobody tagDepositorInsertGFP-Flag-Nup62-EPEA
TagsEPEA, Flag, and GFPExpressionMammalianPromoterT7/CMVAvailable SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only