Addgene

We narrowed to 18,315 results for: Tec

Showing: 2161 - 2180 of 18315 results

Results per page:

Sort results by:

pET-gSTEP1

Plasmid

#159376

Purpose

Expresses gSTEP1 in E. coli

Depositor

Roberto Chica

Article

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

gSTEP1

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET11a+CYP119

Plasmid

#66131

Purpose

Expresses the wild type of CYP119

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLZ12Km2-P23R:TA:ffluc

Plasmid

#88900

Purpose

Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

Depositor

Thomas Proft

Article

Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

Insert

toxin-antitoxin + firefly luciferase

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLH-sgRNA1-2XboxB

Plasmid

#75391

Purpose

sgRNA1-2XboxB

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-2XboxB

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pNH051

Plasmid

#42157

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

GFP-RT-GFP

Use

Tags

Expression

Mutation

Promoter

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
Cas9m4-VP64

Plasmid

#47319

Purpose

Cas9m4 Activator

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

Cas9m4-VP64

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCVII-tdTomato

Plasmid

#162752

Purpose

Retroviral (MSCV) expression of td Tomato fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

tdTomato

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
JD630

Plasmid

#160394

Purpose

Vitis GRF4-GIF1 in pDONR-zeo

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

Vitis GRF4-GIF1 chimera

Use

Entry clon

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-NLS-M-MLV-RT

Plasmid

#181804

Purpose

pET28a-NLS-M-MLV RT

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

NLS-M-MLV-RT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG-Luc-N

Plasmid

#58790

Purpose

Express LucN alone in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

firefly Luciferase 1-398

Use

Tags

FLAG

Expression

Mammalian

Mutation

T576C,T591C to destroy MfeI and EcoRI sites

Promoter

CMV

Available since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
PlayBack-Ef1aKpn1

Plasmid

#203307

Purpose

For expression of eukaryotic genes of interest with Ef1a promoter with Kpn1 as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKD182

Plasmid

#90952

Purpose

Expresses ThsS under the Ptac promoter

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Insert

ThsS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBad33_ISYmu1_reRNA

Plasmid

#205358

Purpose

Bacterial expression of ISYmu1 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISYmu1 TnpB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBbi-BN

Plasmid

#60518

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and neomycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Tags

Expression

Mammalian

Mutation

Promoter

EF1a/RPBSA

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
K-SPOTIT1.0

Plasmid

#171215

Purpose

Expresses a kappa-opioid receptor-based circular permuted green fluorescent protein sensor

Depositor

Wenjing Wang

Article

Kroning et al Angew Chem Int Ed Engl. 2021 Mar 4. doi:

Insert

K-SPOTIT1.0

Use

Lentiviral

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MOBE3

Plasmid

#219943

Purpose

Mammalian expression of MOBE3 protein (apt-CBE-SL3_PT2A_apt-ABE8e)

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

apt-CBE-SL3_PT2A_apt-ABE8e

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p104_gRNA_Sp_CD3e

Plasmid

#213780

Purpose

Expresses CRISPRi gRNA for CD3e promoter and GFP. gRNA driven by mU6.

Depositor

Howard Chang

Article

Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

Sp gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-p4X

Plasmid

#75289

Purpose

Expresses proteins in the periplasm as fusions to a high-binding mutant (A313V) maltose-binding protein

Depositor

Paul Riggs

Article

Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

Type

Empty backbone

Use

Tags

maltose-binding protein

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 19, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBad33_ISEc41_reRNA

Plasmid

#205341

Purpose

Bacterial expression of ISEc41 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISEc41 TnpB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBad33_ISEc46_reRNA

Plasmid

#205343

Purpose

Bacterial expression of ISEc46 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISEc46 TnpB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge