Addgene

We narrowed to 9,706 results for: yeast

Showing: 3241 - 3270 of 9706 results

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pGADT7 Hsh155 R294L

Plasmid

#87293

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 R294L

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

GAL4 AD

Expression

Bacterial and Yeast

Mutation

R294L

Promoter

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 P369E

Plasmid

#87301

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 P369E

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

P369E

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 K335T

Plasmid

#87300

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 K335T

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

K335T

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 K335N

Plasmid

#87298

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 K335N

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

K335N

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 K335E

Plasmid

#87297

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 K335E

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

K335E

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 R294C

Plasmid

#87292

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 R294C

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

R294C

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 E291D

Plasmid

#87290

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 E291D

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

E291D

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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Ppms028(JBEI-11601)

Plasmid

#87958

Purpose

jStack yeast assembly into plant vectors, Level 1 Intermediary Vector, p15A origin

Depositor

Dominique Loque

Article

Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPMS008 (JBEI-11596)

Plasmid

#87957

Purpose

jStack yeast assembly into plant vectors, Level 1 Intermediary Vector, ColE1origin

Depositor

Dominique Loque

Article

Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pms5102(JBEI-10536)

Plasmid

#87969

Purpose

jStack yeast assembly into plant vectors

Depositor

Dominique Loque

Article

Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

Insert

{L_AtHsp18.2}

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pms5055(JBEI-10543)

Plasmid

#87962

Purpose

jStack yeast assembly into plant vectors

Depositor

Dominique Loque

Article

Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

Insert

{L_tOcs}

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pms5092(JBEI-14430)

Plasmid

#87968

Purpose

jStack yeast assembly into plant vectors

Depositor

Dominique Loque

Article

Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

Insert

{T_AtHsp18.2}

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pms5056(JBEI-10534)

Plasmid

#87963

Purpose

jStack yeast assembly into plant vectors

Depositor

Dominique Loque

Article

Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

Insert

{T_tNos}

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pH3F-PYK1

Plasmid

#85774

Purpose

N-ICE plasmid pH3F-PYK1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pGP4GY-cccdB

Plasmid

#83878

Purpose

Destination vector for EYFP fusions integrated at yeast Trp locus, expression from the GPD promoter

Depositor

Jennifer Nemhauser

Article

Pierre-Jerome et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Type

Empty backbone

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-VN155(I153L)-TRP1

Plasmid

#53193

Purpose

chromosomal tagging with Venus

Depositor

John Pringle

Article

Onishi et al J Cell Biol. 2013 Jul 22;202(2):311-29.

Insert

Venus

Use

Yeast genomic targeting

Tags

TRP1

Expression

Mutation

aa 1–155, I to L mutation at aa 153

Promoter

Available since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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pSS63

Plasmid

#47951

Purpose

rtTA positive feedback loop with single tet operator in the GAL1 promoter

Depositor

Attila Becskei

Article

Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

Insert

rtTA

Use

Tet inducible expression

Tags

Expression

Yeast

Mutation

Promoter

T3

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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413-Met25 Kar2 TorsinADE E171Q HDEL

Plasmid

#32124

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Use

Tags

HDEL and Kar2

Expression

Yeast

Mutation

deletion of E at 302 or 303, E171Q

Promoter

Available since

Oct. 31, 2011

Availability

Academic Institutions and Nonprofits only
413-Met25 Kar2 TorsinADE K108A GFP

Plasmid

#32126

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Use

Tags

GFP and Kar2

Expression

Yeast

Mutation

deletion of E at 302 or 303, K108A

Promoter

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
413-Met25 Kar2 TorsinADE K108A HDEL

Plasmid

#32122

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Use

Tags

HDEL and Kar2

Expression

Yeast

Mutation

deletion of E at 302 or 303, K108A

Promoter

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pPS1701

Plasmid

#8852

Depositor

Pamela Silver

Article

Kahana et al Mol Biol Cell. 1998 Jul . 9(7):1741-56.

Insert

NIP100 (NIP100 Budding Yeast)

Use

Tags

3x HA

Expression

Yeast

Mutation

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pHES936

Plasmid

#89199

Purpose

Expresses DIG1 under control of GAL1 promoter

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

RST1 (DIG1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Availability

Academic Institutions and Nonprofits only

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pHES938

Plasmid

#89200

Purpose

Expresses MSG5 under control of GAL1 promoter

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

MSG 5 (MSG5 Budding Yeast)

Use

Yeast integration

Tags

Expression

Mutation

Promoter

GAL1

Available since

Availability

Academic Institutions and Nonprofits only

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pHES939

Plasmid

#89201

Purpose

Expresses GPA1 under control of GAL1 promoter

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

GPA1 (GPA1 Budding Yeast)

Use

Yeast integration

Tags

Expression

Mutation

Promoter

GAl1

Available since

Availability

Academic Institutions and Nonprofits only

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pHES940

Plasmid

#89202

Purpose

Expresses STE4 under control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

STE4 (STE4 Budding Yeast)

Use

Yeast integration

Tags

Expression

Mutation

Promoter

GAl1

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416-yZ3EV-Z3pr-yEGFP (RB3579)

Plasmid

#69100

Purpose

URA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr driving expression of yEGFP

Depositor

David Botstein

Inserts

yZ3EV cassette
Z3 promoter
yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMZ379

Plasmid

#74215

Purpose

Combined sgRNA/Cas9 vector with NatMX marker

Depositor

Mikel Zaratiegui

Inserts

Cas9
rrk1:sgRNA

Use

CRISPR

Tags

Expression

Yeast

Mutation

Silent muation of the CspCI site

Promoter

adh1 and rrk1

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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pRS416-PHO5-GFP-Opi1 + terminator

Plasmid

#58836

Purpose

Expresses GFP-Tagged Opi1 + Terminator in yeast

Depositor

Tim Levine

Insert

Opi1p (OPI1 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

PHO5

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
pRS415-IVa

Plasmid

#31460

Depositor

Ana Kitazono

Article

Kitazono et al Gene. 2011 Jun 22. ():.

Insert

linker

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pMZ377

Plasmid

#74214

Purpose

Combined sgRNA/Cas9 vector with LEU2 marker

Depositor

Mikel Zaratiegui

Inserts

Cas9
rrk1:sgRNA

Use

CRISPR

Tags

Expression

Yeast

Mutation

Silent muation of the CspCI site

Promoter

adh1 and rrk1

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge