166,033 results

1. TFORF2908

   Plasmid

   #143663

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NFIC (NFIC Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSynSRE-T-Luc

   Plasmid

   #60444

   Purpose

   Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.

   Depositor

   Timothy Osborne

   Article

   Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.

   Insert

   HMG-CoA synthase promoter (-324/-225)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Promoter

   HMG-CoA synthase (-324/-225) & TATAA (-28/+39)

   Available Since

   Feb. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a MDHP_YEAST

   Plasmid

   #204233

   Purpose

   Bacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MDHP_YEAST (MDH3 Budding Yeast)
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   The last 3 C-terminal residues (SKL) are the pero…

   Promoter

   Lac

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. mRFP-Rab7

   Plasmid

   #14436

   Purpose

   Mammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.

   Depositor

   Ari Helenius

   Article

   Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

   Insert

   Rab 7 (RAB7A Canis familialis)
   

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   3 silent mutations near the C-terminus

   Available Since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEB2-E2-Crimson

   Plasmid

   #104010

   Purpose

   Plasmid encoding E2-Crimson

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   E2-Crimson
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to DsRed (R2D, S4T, K5E, E10P,…

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3 Flag DLC1

   Plasmid

   #24265

   Depositor

   Roger Davis

   Article

   Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

   Insert

   DLC1 (DYNLL1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   June 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENN.AAV.CAG.Flex.CaMPARI.WPRE.SV40

   Plasmid

   #100831

   Purpose

   AAV expression of CaMPARI, a photoconvertible fluorescent protein-based calcium integrator

   Depositor

   Loren Looger, Eric Schreiter

   Article

   Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

   Insert

   CaMPARI
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAcCas12nHS_RV6_empty

   Plasmid

   #203811

   Purpose

   Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.

   Depositor

   Quanjiang Ji

   Article

   Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

   Insert

   AcCas12nHS_RV6
   

   Use

   CRISPR

   Promoter

   CMV

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Luciferase-pcw107-V5

   Plasmid

   #64649

   Purpose

   (control) when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   n/a
   

   Use

   Lentiviral

   Tags

   V5

   Promoter

   PGK

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pCAGGS-FuG-B2

    Plasmid

    #67513

    Purpose

    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B2 envelope coating virus particle with HiRet by using this plasmid.

    Depositor

    Kazuto Kobayashi

    Article

    Kato et al J Neurosci. 2011 Nov 23;31(47):17169-79.

    Insert

    Fusion Glycoprotein type B2
    

    Use

    Lentiviral

    Tags

    Fusion protein of Vesicular stomatitis Indiana vi…

    Promoter

    CAGGS

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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