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SPOP-V5 (BTB+BACK)

Plasmid

#169723

Purpose

Mammalian Expression of mouse SPOP-BTB+BACK (L186D, L190D, L193D, I217K, Y353E)

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Tags

V5

Expression

Mammalian

Mutation

L186D, L190D, L193D, I217K, Y353E

Promoter

CMV

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS

Plasmid

#78251

Purpose

Can be used to subclone in tagged gene of interest into inducible motor neuron expression vector.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Inserts

-3mnx1 (mnx1 Zebrafish)
-loxP-mTagBFP2-stop-
mCherry-NS-MCS

Use

Zebrafish motor neuron inducible expression

Promoter

-3mnx1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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ACTN3-pEGFP

Plasmid

#52670

Purpose

Overexpression of GFP-tagged α-actinin-3

Depositor

Johannes Hell

Article

Hall et al Neuron. 2013 May 8;78(3):483-97. doi: 10

Insert

ACTN3 (ACTN3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR221-YAP1

Plasmid

#79503

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

YAP1 (YAP1 Human)

Use

Multisite gateway entry clone

Tags

FLAG

Promoter

no Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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OptoProfilin

Plasmid

#208286

Purpose

Expresses Cry2(1-531)-mCherry-Profilin.WT

Depositor

Robert Hughes

Article

Mann et al Chembiochem. 2024 May 2;25(9):e202400007

Insert

Cry2 - mCherry - Profilin.WT (Cry2, PRF1 Mouse, Mustard Weed)

Tags

mCherry

Expression

Mammalian

Mutation

Profilin WT (Mus musculus)

Promoter

CMV

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLV CMV mCherry-DDX21-V5

Plasmid

#175163

Purpose

Lentiviral expression of human DDX21-V5

Depositor

Larry Gerace

Article

Hammond et al J Mol Biol. 2018 Feb 16;430(4):537-553.

Insert

DDX21 (DDX21 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mKate2

Plasmid

#162624

Purpose

Allows for transcription of mKate2 control for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mKate2

Expression

Mammalian

Promoter

SP6

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-mCherry-CLCN3

Plasmid

#225514

Purpose

Lentiviral mCherry-CLCN3 expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

CLCN3 (CLCN3 Human)

Use

Lentiviral

Tags

mCherry

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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OPEN-HLA-B*07:02-BirA

Plasmid

#239752

Purpose

E. coli expression of BirA tagged HLA-B*07:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-B*07:02 (HLA-B Human)

Tags

BirA substrate peptide

Expression

Bacterial

Mutation

G120C

Promoter

T7

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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OPEN-HLA-A*11:01-BirA

Plasmid

#235135

Purpose

E. coli expression of BirA tagged HLA-A*11:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*11:01 (HLA-A Human)

Tags

BirA

Expression

Bacterial

Mutation

G120C

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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