We narrowed to 7,040 results for: poly
-
Plasmid#121767PurposepMVP L3-L2 entry plasmid, contains eCFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest.DepositorInserteCFP + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_R89A-PolyA
Plasmid#112291PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) R89A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralMutationHuman YAP1-2gamma with Arginine 89 to AlaninePromoterEF-1 alphaAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_L91A-PolyA
Plasmid#112292PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) L91A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralMutationHuman YAP1-2gamma with Leucine 91 to AlaninePromoterEF-1 alphaAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_F95A-PolyA
Plasmid#112293PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) F95A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralMutationHuman YAP1-2gamma with Phenylalanine 95 to AlaninePromoterEF-1 alphaAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-poly(alanylglycine)
Plasmid#67003PurposeExpresses malE-poly(alanylglycine) in E. coliDepositorInsertmalE-poly(alanylglycine)
TagsHis6 and malE-Factor XaExpressionBacterialPromotertacAvailable SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-poly-EAK9
Plasmid#67010PurposeExpresses malE-poly-EAK9 in E. coliDepositorInsertmalE-poly-EAK9
TagsHis6 and malE-Factor XaExpressionBacterialPromotertacAvailable SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 RLUC POLIRES FLUC
Plasmid#45642PurposeBicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.DepositorInsertRLUC - IRES - FLUC
UseLuciferaseExpressionMammalianAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-enCas9-PolI3M-TBD
Plasmid#113077PurposeExpresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI3M-TBD
ExpressionBacterialAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-nCas9(D10A)-PolI5MΔ
Plasmid#249069PurposeExpresses nucleus-localized nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.DepositorInsertnCas9-PolI5MΔ
UseCRISPRTagsmCherryExpressionMammalianMutationdeletion of PolI5M N-terminal flap endonuclease d…PromoterCMVAvailable SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-HA_aa 1-420 POLI
Plasmid#201582PurposeExpresses the catalytic domain (aa 1-420) of human DNA polymerase iota with a HA tag in mammalian cellsDepositorInsertDNA polymerase iota (POLI Human)
TagsHAExpressionMammalianMutationExpresses amino acids 1-420 of DNA polymerase iotaAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRM919_WT aa 1-419 His POLI
Plasmid#201445PurposeLow copy vector for expressing the catalytic domain (amino acids 1-419) of human DNA polymerase iota in E. coli with an N-terminal 6x His tag. C-terminal truncation mutant of pJRM919_WT His POLIDepositorInsertDNA polymerase iota (POLI Human)
Tags6x HisExpressionBacterialMutationExpresses amino acids 1-449 of DNA polymerase iot…Available SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-mCherry-nCas9-PolI5M
Plasmid#249066PurposeExpresses nucleus-localized nCas9 (D10A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.DepositorInsertnCas9-PolI5M
UseCRISPRExpressionMammalianAvailable SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_RKK/AAA POLI
Plasmid#131240PurposeMammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations R524A, K526A and K530ADepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains point mutations that convert arginine re…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_3KA + S580A POLI
Plasmid#131241PurposeMammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A, K446A and S580ADepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains point mutations that convert Pol iota se…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_1-590 POLI
Plasmid#131233PurposeMammalian expression of DNA polymerase iota residues 1-590 with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains a 24 bp insertion within the Pol iota co…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_1-420 POLI
Plasmid#131234PurposeMammalian expression of DNA polymerase iota residues 1-420 with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains a 24 bp insertion within the Pol iota co…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Polymerase expression - pCMV-Phi29 (LM2708)
Plasmid#208965PurposeUnfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.DepositorInsertPhi29-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationPhi29(-exo;D169A)PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-flag-hKDM4A(H188A)-polyA
Plasmid#101052Purposein vitro transcription (T7 promoter)DepositorAvailable SinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
AP1muA-Halo-ALFA-polyA-G418 HR
Plasmid#229681PurposeHomology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.DepositorAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
LncRNA overexpression EF1α-BbsI-SV40polyA_puroR
Plasmid#219826PurposeOverexpression vector for non-coding RNAs driven by EF1α promoter with puromycin resistance. Cloning using BbsI.DepositorTypeEmpty backboneExpressionMammalianPromoterEF1aAvailable SinceJuly 29, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits