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Addgene

We narrowed to 3,314 results for: GCT

Showing: 401 - 440 of 3314 results
  1. mU6-sgSik3_1st-hU6-sgNT

    Plasmid
    #177214
    Purpose
    Expresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Insert
    sgSik3_1st/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. SGEP-sh-Hs-PHGDH-1956

    Plasmid
    #188670
    Purpose
    shRNA
    Depositor
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pCfb13244

    Plasmid
    #219915
    Purpose
    The base plasmid of TUNEYALI for TF54
    Depositor
    Insert
    Contains gRNA targeting TF54 (YALI1_F26367g) and homologous arm matching TF54
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pT2/shαSMA/GFP4_Seq2/clone1

    Plasmid
    #201402
    Purpose
    Knockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Insert
    alpha-SMA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. SGEP-sh-Hs-PHGDH-1968

    Plasmid
    #188673
    Purpose
    shRNA
    Depositor
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. SGEP-sh-Hs-PHGDH-1957

    Plasmid
    #188674
    Purpose
    shRNA
    Depositor
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. SGEP-sh-Hs-PHGDH-1967

    Plasmid
    #188672
    Purpose
    shRNA
    Depositor
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. mU6-sgNeo1-hU6-sgNT

    Plasmid
    #177211
    Purpose
    Expresses a Neomycin-targeting gRNA (mU6), a non-targeting gRNA (hU6) and Cre-recombinase
    Depositor
    Insert
    sgNeo/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pSC49

    Plasmid
    #104823
    Purpose
    CRISPR/Cas9 2xplex gRNA targeting Glyma17g11235 (Dcl4a). Also expresses Cas9 from AtUBQ10 promoter.
    Depositor
    Insert
    Glyma17g11235
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. pHnS 2comp Donor;smFP-V5 KO;Gphn#2

    Plasmid
    #240308
    Purpose
    Donor:smFP-V5 KO:Gphn#2
    Depositor
    Insert
    KO gRNAs for Gphn
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
  11. pICSL4723_ CUL3

    Plasmid
    #126891
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
  12. pICSL4723_ HDT701

    Plasmid
    #126886
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
  13. pENTRY4_ CUL3

    Plasmid
    #126903
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 16, 2019
    Availability
    Academic Institutions and Nonprofits only
  14. pENTRY4_ OBfold

    Plasmid
    #126901
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  15. pICSL4723_ OBfold

    Plasmid
    #126889
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  16. pENTRY4_ HDT701

    Plasmid
    #126898
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  17. pENTRY4_ Dja6_1

    Plasmid
    #126894
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  18. pNSU299_GAL2

    Plasmid
    #166088
    Purpose
    Plasmid for constituive spCas9 and tet-inducible GAL2 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Insert
    GAL2 (GAL2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
  19. pLKO.1-TRC.mKO2_shHuR CDS

    Plasmid
    #110426
    Purpose
    TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene and express monomeric Kusabira-Orange2.
    Insert
    ELAVL1 HuR
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (RNA Pol III)
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  20. bU6-sgNeo1-mU6-sgNT-hU6-sgSnrk

    Plasmid
    #177236
    Purpose
    Expresses neomycin (bU6), non-targeting (mU6) and Snrk (hU6) gRNAs and Cre-recombinase
    Depositor
    Insert
    sgNeo1/sgNT1/sgSnrk
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
  21. bU6-sgNeo1-mU6-sgNuak1-hU6-sgNuak2

    Plasmid
    #177234
    Purpose
    Expresses Neomycin (bU6), Nuak1 (mU6) and Nuak2(hU6) gRNAs and Cre-recombinase
    Depositor
    Insert
    sgNeo1/sgNuak1/sgNuak2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
  22. bU6-sgNeo1-mU6-sgAmpk1-hU6-sgAmpk2

    Plasmid
    #177233
    Purpose
    Expresses Neomycin (bU6), Ampk1 (mU6) and Ampk2(hU6) gRNAs and Cre-recombinase
    Depositor
    Insert
    sgNeo1/sgPrkaa1/sgPrkaa2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  23. bU6-sgNeo1-mU6-sgNT-hU6-sgLkb1_1st

    Plasmid
    #177227
    Purpose
    Expresses Neomycin(bU6), non-targeting (mU6) and Lkb1 (hU6) gRNAs and Cre-recombinase
    Depositor
    Insert
    sgNeo1/sgNT1/sgLkb1_1st
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  24. pML107-ARB1

    Plasmid
    #232888
    Purpose
    Plasmid expressing Cas9 and gRNA CAAAAACTAACTGCTTACGG which targets the ARB1 gene.
    Insert
    ARB1 gRNA (ARB1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  25. pML107-YFR054C

    Plasmid
    #232900
    Purpose
    Plasmid expressing Cas9 and gRNA GCTCAAAGAAACAATTAGAG which targets the YFR054C gene.
    Insert
    YFR054C gRNA (YFR054C Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
  26. pML107-SRP14

    Plasmid
    #232896
    Purpose
    Plasmid expressing Cas9 and gRNA GAAAACAAAATGCTCAACAG which targets the SRP14 gene.
    Insert
    SRP14 gRNA (SRP14 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
  27. bU6-sgLkb1_1st-mU6-sgNeo1-hU6-sgNT

    Plasmid
    #177225
    Purpose
    Expresses Lkb1(bU6), neomycin (mU6) and non-targeting(hU6) gRNAs and Cre-recombinase
    Depositor
    Insert
    sgLkb1_1st/sgNeo1/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  28. bU6-sgNeo1-mU6-sgLkb1_1st-hU6-sgNT

    Plasmid
    #177226
    Purpose
    Expresses Neomycin (bU6), Lkb1 (mU6) and non-targeting(hU6) gRNAs and Cre-recombinase
    Depositor
    Insert
    sgNeo1/sgLkb1_1st/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  29. pUPD2b:TMV-3NTR

    Plasmid
    #106218
    Purpose
    Provides Nos terminator and 3' part of Tobacco mosaic virus (3NTR, ribozyme) as level 0 GoldenBraid part (GCTT-CGCT)
    Depositor
    Insert
    Infectious clone of Tobacco mosaic virus, 3' part
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  30. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid
    #140082
    Purpose
    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter
    Depositor
    Insert
    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    Use
    In vitro transcription (mrna synthesis)
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
  31. pX459-puro-hMYC

    Plasmid
    #185376
    Purpose
    For mammalian expression of guide RNA: TGCTGCCAAGAGGGTCAAGT that targets human MYC
    Depositor
    Insert
    MYC (MYC Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
  32. pZSCAN9.1.0-gDNA

    Plasmid
    #112440
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF193
    Depositor
    Insert
    ZNF193 (ZNF193 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
  33. pZBTB40.1.0-gDNA

    Plasmid
    #112431
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZBTB40
    Depositor
    Insert
    ZBTB40 (ZBTB40 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
  34. pZNF398.2.0-gDNA

    Plasmid
    #112415
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF398
    Depositor
    Insert
    ZNF398 (ZNF398 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
  35. pZNF70.1.0-gDNA

    Plasmid
    #112414
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF70
    Depositor
    Insert
    ZNF70 (ZNF70 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
  36. pLKO.1-blast shLuc

    Plasmid
    #110409
    Purpose
    shLuc (Target TTACGCTGAGTACTTCGA), silence LUC gene, blasticidin selection.
    Insert
    Luciferase
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (RNA PolIII)
    Available since
    Nov. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  37. pYTP MYRF sg3

    Plasmid
    #88859
    Purpose
    pAC154-dual-dCas9VP160-sgExpression with MYRF sg3 cloned into BbsI site
    Depositor
    Insert
    dCas9VP160 and MYRF sg3 gRNA (MYRF Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
  38. pYTP MYRF sg6

    Plasmid
    #88862
    Purpose
    pAC154-dual-dCas9VP160-sgExpression with MYRF sg6 cloned into BbsI site
    Depositor
    Insert
    dCas9VP160 and MYRF sg6 gRNA (MYRF Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 401 - 440 of 3314 results