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Showing: 401 - 440 of 8763 results

  1. pSV40-GUS-P2A-Hygro

    Plasmid
    #106480
    Purpose
    Expression of GUS and Hygromycin resistance gene from SV40 promoter
    Depositor
    Alex Hewitt
    Inserts
    beta-glucuronidase
    Hygromycin B resistance
    Expression
    Mammalian and Plant
    Promoter
    SV40 promoter
    Available Since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106480-plasmid-map-sequence-id-207984

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  2. LHP938 - Gal1-HA-Ub

    Plasmid
    #32175
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Tags
    HA
    Expression
    Yeast
    Promoter
    Gal1
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  3. mCherry-Paxillin-KM-N-18

    Plasmid
    #55115
    Purpose
    Localization: Focal Adhesions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Paxillin KM
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Mutation to remove Kozak sequence
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  4. LHP336 - HA-Ub-K48R

    Plasmid
    #32164
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Tags
    HA
    Expression
    Yeast
    Mutation
    K48R
    Promoter
    CUP1
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  5. mApple-PDHA1-N-10

    Plasmid
    #54938
    Purpose
    Localization: Mitochondria, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    PDHA1 (PDHA1 Human)
    Tags
    mApple
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. pBS L30 mRuby3-Rab11A

    Plasmid
    #166430
    Purpose
    Rab11A fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.
    Depositor
    Raphael Gaudin
    Insert
    Rab11A
    Tags
    mRuby3
    Expression
    Mammalian
    Promoter
    L30 weak promoter
    Available Since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    166430-plasmid-map-sequence-id-333541

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  7. pGL3-Basic-OPN-600

    Plasmid
    #11997
    Depositor
    Steven Johnson
    Insert
    Osteopontin (SPP1 Human)
    Expression
    Mammalian
    Mutation
    The following construct contains the OPN promoter…
    Available Since
    July 25, 2007
    Availability
    Academic Institutions and Nonprofits only

  8. mApple-Paxillin-KM-N-18

    Plasmid
    #54936
    Purpose
    Localization: Focal Adhesions, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Paxillin-KM
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Mutation to remove Kozak sequence
    Promoter
    CMV
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. pJK554

    Plasmid
    #72453
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72453-plasmid-map-sequence-id-133893

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  10. LHP599 - pACT2-Ub

    Plasmid
    #32170
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Tags
    Gal4 AD and HA
    Expression
    Yeast
    Promoter
    pADH1
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  11. LPH2776 - Ub-I44A

    Plasmid
    #32163
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Expression
    Yeast
    Mutation
    I44A
    Promoter
    CUP1
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  12. CAA705

    Plasmid
    #250781
    Purpose
    Expression of a TOP2B C-terminal domain-GST fusion protein
    Depositor
    Caroline Austin
    Insert
    TOP2B C-terminal domain (TOP2B Human)
    Tags
    GST
    Expression
    Bacterial
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250781-plasmid-map-sequence-id-499030

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  13. pTYB1-T4denV

    Plasmid
    #228216
    Purpose
    Expression in BL21(DE3) of T4 endonuclease V N-glycosylase UV repair enzyme
    Depositor
    Stephen Benkovic
    Insert
    denV
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    228216-plasmid-map-sequence-id-455996

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  14. L0I101- GG4-pGATA23-Bxb1-tUBQ10-GG6

    Plasmid
    #219712
    Purpose
    Level 0 pGATA23-Bxb1-tUBQ10 construct for assembly of two integrase constructs, GG4 and GG6 BsaI sites.
    Depositor
    Jennifer Nemhauser
    Insert
    GG4-pGATA23-Bxb1-tUBQ10-GG6
    Use
    Synthetic Biology
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219712-plasmid-map-sequence-id-435863

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  15. L0I90 - GG0-pSPCH-PhiC31-tUBQ1-GG4

    Plasmid
    #219709
    Purpose
    Level 0 pSPCH-PhiC31-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pSPCH:PhiC31:tUBQ1-GG4
    Use
    Synthetic Biology
    Available Since
    June 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    219709-plasmid-map-sequence-id-435862

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  16. pcDNA-HisFlag-rUSP19ER-CRmut

    Plasmid
    #155253
    Purpose
    for mammalian overexpression of the mutated ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (ER isoform) CR mutant (Usp19 Rat)
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Cys(545) to Arg
    Promoter
    CMV
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155253-plasmid-map-sequence-id-311950

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  17. pEGFP-rUSP19CYT-CAmut

    Plasmid
    #155250
    Purpose
    for mammalian overexpression of the mutated CYT isoform of rat USP19 fused to GFP at N-terminus
    Depositor
    Simon Wing
    Insert
    USP19 (Cytoplasmic isoform) CA mutant (Usp19 Rat)
    Expression
    Mammalian
    Mutation
    Cys(545) to Ala
    Promoter
    CMV
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155250-plasmid-map-sequence-id-311949

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  18. pEGFP-rUSP19ER-CAmut

    Plasmid
    #155249
    Purpose
    for mammalian overexpression of the mutated ER isoform of rat USP19 fused to GFP at N-terminus
    Depositor
    Simon Wing
    Insert
    USP19 (ER isoform) CA mutant (Usp19 Rat)
    Expression
    Mammalian
    Mutation
    Cys(545) to Ala
    Promoter
    CMV
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155249-plasmid-map-sequence-id-311947

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  19. pcDNA-HisFlag-rUSP19ER deltaN3

    Plasmid
    #155243
    Purpose
    for mammalian overexpression of truncated (delta1-1631) ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (delta1-1631) (ER isoform) (Usp19 Rat)
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155243-plasmid-map-sequence-id-311880

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  20. pcDNA-HisFlag-rUSP19ER deltaN2

    Plasmid
    #155242
    Purpose
    for mammalian overexpression of truncated (delta1-1088) ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (delta1-1088) (ER isoform) (Usp19 Rat)
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155242-plasmid-map-sequence-id-311878

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  21. pcDNA-HisFlag-rUSP19ER deltaN1

    Plasmid
    #155241
    Purpose
    for mammalian overexpression of truncated (delta1-536) ER isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (delta1-536) (ER isoform) (Usp19 Rat)
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155241-plasmid-map-sequence-id-311860

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  22. pRARB.1.0-gDNA

    Plasmid
    #132471
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    RARB (RARB Human)
    Use
    CRISPR
    Available Since
    Dec. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    132471-plasmid-map-sequence-id-267178

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  23. pJK275

    Plasmid
    #73363
    Purpose
    Encodes Crepis alpina delta-8 sphingolipid desaturase (SLD1)
    Depositor
    T. J. Kappock
    Insert
    delta-8 sphingolipid desaturase
    Expression
    Yeast
    Mutation
    missing residues 1-6
    Promoter
    GAL1
    Available Since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73363-plasmid-map-sequence-id-138870

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  24. pJK521

    Plasmid
    #72440
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes (AaOrfX)
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein
    Use
    Unspecified
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    n/a
    Available Since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72440-plasmid-map-sequence-id-133778

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  25. pJK323

    Plasmid
    #73495
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes and N5-carboxyaminoimidazole ribonucleotide synthetase
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Expression
    Bacterial
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73495-plasmid-map-sequence-id-135755

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  26. pJK536

    Plasmid
    #72444
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes (AaOrfX)
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein
    Expression
    Bacterial
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    T7
    Available Since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72444-plasmid-map-sequence-id-136729

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  27. pJK550

    Plasmid
    #72450
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    inserted Val2
    Promoter
    T7
    Available Since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72450-plasmid-map-sequence-id-133877

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  28. LHP809 - Ub-K63A-CYC1

    Plasmid
    #32166
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Expression
    Yeast
    Mutation
    K63A
    Promoter
    CUP1
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  29. LHP327 - Ub-F45A/G76A

    Plasmid
    #32165
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Expression
    Yeast
    Mutation
    F45A and G76A
    Promoter
    CUP1
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  32. pET151-D-TOPO-PARP14-SΔK

    Plasmid
    #216658
    Purpose
    Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.
    Depositor
    Herwig Schüler
    Article
    Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.
    Insert
    PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Synthetic, Human)
    Tags
    6xHis-V5-TEV
    Expression
    Bacterial
    Mutation
    Deletion starting from Q1390 to D1448 and substit…
    Promoter
    T7
    Available Since
    Aug. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    216658-plasmid-map-sequence-id-429340

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Showing: 401 - 440 of 8763 results