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Showing: 501 - 550 of 2230 results

  1. pDK714

    Plasmid
    #134230
    Purpose
    Mammalian expresion of tdTomato SKAP
    Depositor
    Iain Cheeseman
    Insert
    DK365 tdTomato (short SKAP) (KNSTRN Human)
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    134230-plasmid-map-sequence-id-272270

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  2. pJK650

    Plasmid
    #72400
    Purpose
    Produces Escherichia coli glutamine synthetase with Y397F mutation that prevents adenylation (EcGlnA-Y397F)
    Depositor
    T. J. Kappock
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Available since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    72400-plasmid-map-sequence-id-133994

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  3. pJK122

    Plasmid
    #74295
    Purpose
    Produces Acetobacter aceti 1023 hypothetical acetic acid resistance protein B, cleavable N-terminal NusA fusion
    Depositor
    T. J. Kappock
    Insert
    hypothetical acetic acid resistance protein B
    Use
    Tags
    NusA
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    74295-plasmid-map-sequence-id-141881

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  4. pDK713

    Plasmid
    #134229
    Purpose
    Mammalian expresion of tdTomato SKAP
    Depositor
    Iain Cheeseman
    Insert
    DK360 tdTomato (long SKAP) (KNSTRN Human)
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    134229-plasmid-map-sequence-id-272275

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  5. pJK237

    Plasmid
    #73339
    Purpose
    Produces Acetobacter aceti 1023 citrate synthase with cleavable N-terminal His6/CBD/Ssp-intein fusion
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Use
    Tags
    His6, Ssp intein, and chitin-binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73339-plasmid-map-sequence-id-138207

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  6. pRDB_268

    Plasmid
    #231112
    Purpose
    SpG-Cas9
    Depositor
    John Doench, David Root
    Insert
    Cas9-SpG
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231112-plasmid-map-sequence-id-458383

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  7. AA566

    Plasmid
    #231115
    Purpose
    Fragmid fragment: (guide cassette) ABE activity-based selection cloning
    Depositor
    John Doench, David Root
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231115-plasmid-map-sequence-id-458370

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  8. AA568

    Plasmid
    #231117
    Purpose
    Fragmid fragment: (guide cassette) CBE activity-based selection cloning
    Depositor
    John Doench, David Root
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231117-plasmid-map-sequence-id-458363

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  9. pJK103

    Plasmid
    #73265
    Purpose
    Produces Acetobacter aceti 1023 hypothetical acetic acid resistance protein B, C-terminal His6 tag (AaAarBH6)
    Depositor
    T. J. Kappock
    Insert
    hypothetical acetic acid resistance protein B
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73265-plasmid-map-sequence-id-134809

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  10. pJK610

    Plasmid
    #72531
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ); large subunit wutg C-terminal His6 tag (AaPurLH6)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7 and n/a
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    72531-plasmid-map-sequence-id-132619

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  11. pJK540

    Plasmid
    #72446
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein with N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7 and n/a
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72446-plasmid-map-sequence-id-133865

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  12. pJK552

    Plasmid
    #72451
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins); N5-carboxyaminoimidazole ribonucleotide synthetase, Val319>Phe mutant (AaPurK-V319F)
    Depositor
    T. J. Kappock
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Val319 to Phe and inserted Val2
    Promoter
    T7
    Available since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    72451-plasmid-map-sequence-id-133883

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  13. pJK141

    Plasmid
    #73316
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with Pas2p signal sequence, c-myc tag, and His6-tag
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Use
    Tags
    His6, Pas2p signal sequence, and c-myc
    Expression
    Yeast
    Mutation
    synthetic gene
    Promoter
    AOX1
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73316-plasmid-map-sequence-id-135024

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  14. pJK586

    Plasmid
    #72459
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Thr/Trp165>Phe mutant (AaPurE1fwf-S57T)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes tryptophan-34 to phenylalanine, …
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72459-plasmid-map-sequence-id-133898

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  15. pJK565

    Plasmid
    #72454
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Ala/Trp165>Phe mutant (AaPurE1fwf-H59A)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes tryptophan-34 to phenylalanine, …
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72454-plasmid-map-sequence-id-133894

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  16. pJK578

    Plasmid
    #72457
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Ala/Trp165>Phe mutant (AaPurE1fwf-S57A)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes tryptophan-34 to phenylalanine, …
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72457-plasmid-map-sequence-id-133895

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  17. pJK585

    Plasmid
    #72458
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Cys/Trp165>Phe mutant (AaPurE1fwf-S57C)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes tryptophan-34 to phenylalanine, …
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72458-plasmid-map-sequence-id-133896

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  18. pJK543

    Plasmid
    #72516
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); large subunit (AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    contains additional Ala2
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72516-plasmid-map-sequence-id-132549

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  19. pJK576

    Plasmid
    #72527
    Purpose
    Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); hypothetical protein associated with purSyQL cluster (AaOrfY)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    Use
    Gene synthesis
    Tags
    Expression
    Mutation
    synthetic gene
    Promoter
    n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72527-plasmid-map-sequence-id-132615

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  20. pJK602

    Plasmid
    #71607
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Tyr154>Phe mutant (AaPurE1-H59N,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine, tyrosine-154 …
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71607-plasmid-map-sequence-id-131684

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  21. pJK603

    Plasmid
    #71608
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Phe mutant (AaPurE1fww-Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tyrosine-…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71608-plasmid-map-sequence-id-131687

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  22. pJK663

    Plasmid
    #71612
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Tyr154>Phe/Trp165>Phe mutant (AaPurE1wwf-Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tyrosine-154 to phenylalanine, tryptophan…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71612-plasmid-map-sequence-id-131690

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  23. pJK665

    Plasmid
    #71614
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Trp97>Tyr mutant (AaPurE1fyw-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, histidine…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71614-plasmid-map-sequence-id-131692

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  24. pJK510

    Plasmid
    #71600
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Trp165>Phe mutant (AaPurE1wwf-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine, tryptophan-16…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71600-plasmid-map-sequence-id-131674

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  25. pCVD315

    Plasmid
    #63737
    Purpose
    Derivative of pKO-1 carrying the multiple cloning region from M13mp19RF
    Depositor
    James Kaper
    Article
    Vimr et al J Bacteriol. 1988 Apr;170(4):1495-504.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  27. pRDB_270

    Plasmid
    #231111
    Purpose
    C>T base editor
    Depositor
    John Doench, David Root
    Insert
    TadA-CDd-nCas9-SpG-UGI-HA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    D10A
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    231111-plasmid-map-sequence-id-458638

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  28. pRDA_867

    Plasmid
    #231109
    Purpose
    A>G base editor
    Depositor
    John Doench, David Root
    Insert
    ABE8e-nCas9-SpG-HA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    D10A
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231109-plasmid-map-sequence-id-458425

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  37. pJK276

    Plasmid
    #73366
    Purpose
    Encodes Crepis alpina plastid omega-3 fatty acid desaturase (FAD7)
    Depositor
    T. J. Kappock
    Insert
    plastid omega-3 fatty acid desaturase
    Use
    Tags
    Expression
    Yeast
    Mutation
    Mutation I231F. Also insert is missing residues 1…
    Promoter
    GAL1
    Available since
    May 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    73366-plasmid-map-sequence-id-135095

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  38. pJK693

    Plasmid
    #72382
    Purpose
    Produces Escherichia coli cysteine desulfurase IscS with Ala260>Thr mutation, N-terminal His6-tag (H6EcIscS-A260T)
    Depositor
    T. J. Kappock
    Insert
    cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent (iscS Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated alanine-260 to threonine
    Promoter
    T7
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72382-plasmid-map-sequence-id-134008

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  40. pJK142

    Plasmid
    #73317
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) as a fusion to Pas2p, with c-myc tag and His6-tag
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Use
    Tags
    His6, Pas2p, and c-myc
    Expression
    Yeast
    Mutation
    synthetic gene. Encodes a glycine-204 to serine m…
    Promoter
    AOX1
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73317-plasmid-map-sequence-id-140432

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  41. pRDB_092

    Plasmid
    #231110
    Purpose
    C>T base editor
    Depositor
    John Doench, David Root
    Insert
    TadA-CDd-nCas9-SpG-UGI
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    D10A
    Promoter
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231110-plasmid-map-sequence-id-459684

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  42. AA516

    Plasmid
    #231118
    Purpose
    Fragmid fragment: (2A Selection) CBE activity-based selection intron
    Depositor
    John Doench, David Root
    Insert
    PuroN'-intron-PuroC'
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231118-plasmid-map-sequence-id-458371

    Enlarge

  43. AA509

    Plasmid
    #231116
    Purpose
    Fragmid fragment: (2A Selection) ABE activity-based selection intron
    Depositor
    John Doench, David Root
    Insert
    PuroN'-intron-PuroC'
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231116-plasmid-map-sequence-id-458354

    Enlarge

  44. pJK652

    Plasmid
    #72470
    Purpose
    Produces Escherichia coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag, His233>Gly mutant (H6EcYfdE-H233G)
    Depositor
    T. J. Kappock
    Insert
    acetyl-CoA:oxalate CoA-transferase (yfdE Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated histidine-233 to glycine
    Promoter
    T7
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72470-plasmid-map-sequence-id-134018

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  47. pJK520

    Plasmid
    #72479
    Purpose
    Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); hypothetical protein with N-terminal His6 tag (H6AaOrfY), synthetic gene
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    Use
    Gene synthesis
    Tags
    His6
    Expression
    Mutation
    synthetic gene
    Promoter
    n/a and none
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72479-plasmid-map-sequence-id-132532

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  48. pJK559

    Plasmid
    #71603
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tyrosine-…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71603-plasmid-map-sequence-id-131677

    Enlarge

  49. pJK562

    Plasmid
    #71604
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Leu/Trp165>Phe mutant (AaPurE1fwf-Y154L)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tyrosine-…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71604-plasmid-map-sequence-id-131680

    Enlarge

  50. pJK604

    Plasmid
    #71610
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Tyr154>Phe mutant (AaPurE1fww-H59N,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, histidine…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71610-plasmid-map-sequence-id-131686

    Enlarge

Showing: 501 - 550 of 2230 results