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Plasmid#176665PurposeExpression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only
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pCDNA5/FRT-ChemoB-NAD
Plasmid#193824PurposeCMV overexpression of ChemoB-NAD in mammalian cell linesDepositorInsertChemoB-NAD
UseTagsExpressionMammalianMutationEBFP2:N39Y, A206K, T225R; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLib6.6B
Plasmid#176666PurposeExpression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoB
Plasmid#193808PurposeCMV overexpression of ChemoB in mammalian cell linesDepositorInsertChemoB
UseTags10xHisExpressionMammalianMutationEBFP2: N39Y, V206K, T225R; HT7: E143R, E147R, L27…PromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+) ChemoB-CaM
Plasmid#193812PurposeProtein production of ChemoB-CaM in bacteriaDepositorInsertChemoB-CaM
UseTags10xHisExpressionBacterialMutationEBFP2: N39Y, V206KPromoterT7Available sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJM572 ZF6x6-C intC-ZF1-NLS-HA in TUPV2
Plasmid#161559PurposeInducible expression of intC-ZF1-NLS-HA under the ZF6x6-C promoterDepositorInsertintC-ZF1-NLS-HA
UseSynthetic BiologyTagsHAExpressionMammalianMutationPromoterZF6x6-CAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM589 EF1α intN-ZF10-NLS-HA in TUPV2
Plasmid#161565PurposeConstitutive expression of intN-ZF10-NLS-HA under the EF1α promoterDepositorInsertintN-ZF10-NLS-HA
UseSynthetic BiologyTagsHAExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM516 EF1α intC-ZF1-NLS-HA in TUPV2
Plasmid#161551PurposeConstitutive expression of intC-ZF1-NLS-HA under the EF1α promoterDepositorInsertintC-ZF1-NLS-HA
UseSynthetic BiologyTagsHAExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM522 EF1α intC-ZF10-NLS-HA in TUPV2
Plasmid#161552PurposeConstitutive expression of intC-ZF10-NLS-HA under the EF1α promoterDepositorInsertintC-ZF10-NLS-HA
UseSynthetic BiologyTagsHAExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTW087
Plasmid#115935PurposeORF insert for replicon MoCloDepositorInsertLvl0 EBFP2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK331
Plasmid#59572PurposeTC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceIDepositorInsertSGP(16) 2xK-turn Kozak EGFP attB2 AscI
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG::Nucbow
Plasmid#158996PurposeMulticolor nuclear-restricted labeling with a genome-integrating piggyBac transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)DepositorInsertNucbow
UseTagsAll FPs are fused to H2BExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4B-Consensus
Plasmid#176678PurposeExpression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM600 EF1α PC 3xFLAG-FKBP-FGFR4-TEVp in TUPV2
Plasmid#161570PurposeConstitutive expression of PC 3xFLAG-FKBP-FGFR4-TEVp under the EF1α promoterDepositorInsertPC 3xFLAG-FKBP-FGFR4-TEVp
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTSAR3.5t
Plasmid#210257PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertseBFP2
superfolder GFP
UseTagsssraExpressionBacterialMutationPromoteripaH7.8p and rpsMAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4B-Agam_695
Plasmid#176668PurposeExpression of sgRNA under An. gambiae U6-2 (AGAP013695) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4B-Aaeg_774
Plasmid#176673PurposeExpression of sgRNA under Ae. Aegypti U6 (AAEL017774) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4B-Cquin_596
Plasmid#176669PurposeExpression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4B-Aalb_743
Plasmid#176676PurposeExpression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM582 EF1α 3xFLAG-NLS-VP64-ZF2 in TUPV2
Plasmid#161543PurposeConstitutive expression of 3xFLAG-NLS-VP64-ZF2 under the EF1α promoterDepositorInsert3xFLAG-NLS-VP64-ZF2
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only