We narrowed to 10,200 results for: ESP

1. pRDAX-EMMI-029 mNeptune2.5(G-H)//Esp3I

   Plasmid

   #130231

   Purpose

   EMMA Kit # 1000000119 extension

   Depositor

   Gary Lewin

   Insert

   mNeptune2.5
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRDAX-EMMI-030 SuperNovaGreen(G-H)//Esp3I

   Plasmid

   #130232

   Purpose

   EMMA Kit # 1000000119 extension

   Depositor

   Gary Lewin

   Insert

   SuperNovaGreen
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRDAX-EMMI-032 connector(H-K)//Esp3I

   Plasmid

   #130234

   Purpose

   EMMA Kit # 1000000119 extension

   Depositor

   Gary Lewin

   Insert

   artificial connector
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRDAX-EMMI-033 connector(M-W)//Esp3I

   Plasmid

   #130235

   Purpose

   EMMA Kit # 1000000119 extension

   Depositor

   Gary Lewin

   Insert

   artificial connector
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRDAX-EMMI-035 Lamp1(D-G)//Esp3I

   Plasmid

   #130237

   Purpose

   EMMA Kit # 1000000119 extension

   Depositor

   Gary Lewin

   Insert

   Lamp1 (Lamp1 Mouse)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRDAX-EMMI-036 Vimentin(D-G)//Esp3I

   Plasmid

   #130238

   Purpose

   EMMA Kit # 1000000119 extension

   Depositor

   Gary Lewin

   Insert

   Vimentin
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRDAX-EMMI-037 NLSNoLS(D-G)//Esp3I

   Plasmid

   #130239

   Purpose

   EMMA Kit # 1000000119 extension

   Depositor

   Gary Lewin

   Insert

   NLSNoLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRDAX-EMMI-038 Lyn(D-G)//Esp3I

   Plasmid

   #130240

   Purpose

   EMMA Kit # 1000000119 extension

   Depositor

   Gary Lewin

   Insert

   Lyn
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRDAX-EMMI-039 LifeAct(D-G)//Esp3I

   Plasmid

   #130241

   Purpose

   EMMA Kit # 1000000119 extension

   Depositor

   Gary Lewin

   Insert

   LifeAct
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRDAX-EMMI-017 IRES-WT(H-I)//Esp3I

    Plasmid

    #130219

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    IRES wt
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRDAX-EMMI-034 EB3(D-G)//Esp3I

    Plasmid

    #130236

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    EB3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PIESP15-COMP-blac-flag-his

    Plasmid

    #110958

    Purpose

    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

    Depositor

    Gavin Wright

    Article

    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

    Insert

    parasite-infected erythrocyte surface protein (PIESP15) (PF3D7_0103900 Synthetic)
    

    Use

    Tags

    Exogenous signal peptide of mouse origin and rat …

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX459V2.0-eSpCas9(1.1)+K810A+K855A

    Plasmid

    #108299

    Purpose

    pX459 V2.0 (Plasmid #62988) with the K810A, K848A, K855A, K1003A, and R1060A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid

    #182653

    Purpose

    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Inserts

    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    

    Use

    Tags

    HA tag and nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    E55D and Y306A/Y384F (AF) double mutant of Methan…

    Promoter

    CMV and U6

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)_4x(U6 tRNA M15)_CMV NESPylRS(AF)

    Plasmid

    #182652

    Purpose

    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & 4x M15 tRNACUA used for amber codon suppression in mammalian cells

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Inserts

    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    4xU6-tRNAM15
    

    Use

    Tags

    nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Y306A/Y384F (AF) double mutant of Methanosarcina …

    Promoter

    CMV and U6

    Available since

    May 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs

    Plasmid

    #220493

    Purpose

    To generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.

    Depositor

    Sébastien Britton

    Article

    Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

    Insert

    sgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. BPK3258 - human expression plasmid for eSpCas9(1.1)

    Plasmid

    #101176

    Purpose

    Human expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-eSpCas9(1.1)(K848A/K1003A/R1060A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    K848A/K1003A/R1060A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLe6Δ -hLP-mcs/(EGFP-IresPuro-hInt)

    Plasmid

    #64314

    Purpose

    chondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter vector to select iChon cells

    Depositor

    Noriyuki Tsumaki

    Article

    Outani et al PLoS One. 2013 Oct 16;8(10):e77365. doi:

    Inserts

    COL11A2 promoter (COL11A2 Human)
    COL11A2 enhancer (COL11A2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP and IRES-PURO

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRDAX-EMMI-021 IRES-D[C333G](H-I)//Esp3I

    Plasmid

    #130223

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    IRES-D[C333G]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    C333G

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRDAX-EMMI-023 IRES-F[C336A](H-I)//Esp3I

    Plasmid

    #130225

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    IRES-F[C336A]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    C336A

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge