We narrowed to 238 results for: ESP3;

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pVP096

Plasmid

#222043

Purpose

pGGPK-AG2 where the ccdb/CmR has been swappe for mRFP1e; Esp3I domesticated backbone.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

mRFP1e

Use

Synthetic Biology; Greengate compatible cloning v…

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pTn7-SCOUT10

Plasmid

#200989

Purpose

pL0V-Lv1-pUC18R6KT-miniTn7-GG-Esp3I sites for Antb modules, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Expression

Bacterial

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT20

Plasmid

#200990

Purpose

pL0V-Lv2-pUC18R6KT-miniTn7-GG-Esp3I sites for Antb modules, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Expression

Bacterial

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pUPD2+pEstrInd

Plasmid

#170880

Purpose

Phytobrick - Estradiol Inducible Promoter

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

pEstradiol_Inducible

Expression

Plant

Mutation

c.837C>T and c.1353G>A to remove BsaI and E…

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pBlue-TAL

Plasmid

#49401

Purpose

The pBlue-TAL is suitable for TALEN mutagenesis in insects. It is compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites and a T7 promoter.

Depositor

Michal Zurovec

Article

Takasu et al PLoS One. 2013 Sep 18;8(9):e73458. doi:

Type

Empty backbone

Use

TALEN

Expression

Insect

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only

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Lenti-Trono-EFS-Blast-P2A-TurboRFP

Plasmid

#228893

Purpose

Cloning and expression of gRNAs or pegRNAs with EcoRI/Esp3I insertion.

Depositor

Francisco J Sánchez-Rivera

Article

Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pMC037-HisMS2_PLP_pac

Plasmid

#128233

Purpose

Expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

Depositor

Paul Freemont

Article

de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

Inserts

Maturation Protein
Coat Protein Dimer

Expression

Bacterial

Promoter

T7

Available Since

Nov. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2_hMGFP-60A

Plasmid

#200393

Purpose

A plasmid IVT template containing a humanized Monster Green Fluorescent Protein (hMGFP) CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

Depositor

Xiao Wang

Article

Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

Insert

monster GFP

Expression

Mammalian

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2_Renilla_luc-60A

Plasmid

#200397

Purpose

A plasmid IVT template containing a Renilla luciferase CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

Depositor

Xiao Wang

Article

Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

Insert

Renilla luciferase

Expression

Mammalian

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pMC051-HisMS2_PLP_pac_LC

Plasmid

#128234

Purpose

Lower Copy expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

Depositor

Paul Freemont

Article

de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

Inserts

Maturation Protein
Coat Protein Dimer

Expression

Bacterial

Promoter

T7

Available Since

Nov. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pgRNA-backbone

Plasmid

#172517

Purpose

Yeast plasmid encoding a 2-kb gRNA-placeholder flanked by BsmBI/Esp3I restriction sites to facilitate gRNA insertion

Depositor

Leonid Kruglyak

Article

Schubert et al Elife. 2022 Nov 3;11. pii: 79525. doi: 1

Insert

gRNA cassette

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK087

Plasmid

#171359

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

P-ANtRNA[Pro1]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK086

Plasmid

#171358

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

P-ANtRNA[Arg21]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2_Firefly_degron-60A

Plasmid

#200396

Purpose

A plasmid IVT template containing a destabilized Firefly luciferase-PEST (degron) fusion followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

Depositor

Xiao Wang

Article

Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

Insert

Firefly luciferase - degron

Expression

Mammalian

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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EC71100

Plasmid

#154068

Purpose

Level 0 Golden Gate vector, pHvHSP17

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

pHvHSP17

Use

Standard cloning vector used by geneart for synth…

Mutation

All BsaI, Esp3I and BpiI restriction sites were r…

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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EC71022

Plasmid

#159608

Purpose

Level 0.5 Golden Gate Vector, loxP-mCHERRY-HDEL-t35s-loxP

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

loxP-mCherry-HDEL-t35S-loxP

Use

Cre/Lox and Synthetic Biology

Mutation

All BsaI, Esp3I and BPiI restriction sites were r…

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pJG85

Plasmid

#89281

Purpose

Entry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sites

Depositor

Daniel Voytas

Article

Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pEND-350_Cacnes_ori

Plasmid

#225605

Purpose

Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEND-349_Cacnes_ori

Plasmid

#225604

Purpose

Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector with C. acnes chloramphenicol resistance, harbouring a medium-copy pBR322 E. coli ori (no ROP)

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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EC71171

Plasmid

#154069

Purpose

Level 0 Golden Gate vector, CRE with U5 intron at 254bp, in pICH41308 backbone

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

CRE_U5intron

Use

Synthetic Biology

Mutation

All BsaI, Esp3I and BPiI restriction sites were r…

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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