-
Plasmid#222043PurposepGGPK-AG2 where the ccdb/CmR has been swappe for mRFP1e; Esp3I domesticated backbone.DepositorInsertmRFP1e
UseSynthetic Biology; Greengate compatible cloning v…TagsExpressionMutationPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT10
Plasmid#200989PurposepL0V-Lv1-pUC18R6KT-miniTn7-GG-Esp3I sites for Antb modules, ApRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT20
Plasmid#200990PurposepL0V-Lv2-pUC18R6KT-miniTn7-GG-Esp3I sites for Antb modules, ApRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2+pEstrInd
Plasmid#170880PurposePhytobrick - Estradiol Inducible PromoterDepositorInsertpEstradiol_Inducible
UseTagsExpressionPlantMutationc.837C>T and c.1353G>A to remove BsaI and E…PromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlue-TAL
Plasmid#49401PurposeThe pBlue-TAL is suitable for TALEN mutagenesis in insects. It is compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites and a T7 promoter.DepositorTypeEmpty backboneUseTALENTagsExpressionInsectMutationPromoterAvailable sinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Trono-EFS-Blast-P2A-TurboRFP
Plasmid#228893PurposeCloning and expression of gRNAs or pegRNAs with EcoRI/Esp3I insertion.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMC037-HisMS2_PLP_pac
Plasmid#128233PurposeExpression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.DepositorInsertsMaturation Protein
Coat Protein Dimer
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCS2_Renilla_luc-60A
Plasmid#200397PurposeA plasmid IVT template containing a Renilla luciferase CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.DepositorInsertRenilla luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2_hMGFP-60A
Plasmid#200393PurposeA plasmid IVT template containing a humanized Monster Green Fluorescent Protein (hMGFP) CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.DepositorInsertmonster GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC051-HisMS2_PLP_pac_LC
Plasmid#128234PurposeLower Copy expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.DepositorInsertsMaturation Protein
Coat Protein Dimer
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pgRNA-backbone
Plasmid#172517PurposeYeast plasmid encoding a 2-kb gRNA-placeholder flanked by BsmBI/Esp3I restriction sites to facilitate gRNA insertionDepositorInsertgRNA cassette
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK087
Plasmid#171359PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertP-ANtRNA[Pro1]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK086
Plasmid#171358PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertP-ANtRNA[Arg21]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2_Firefly_degron-60A
Plasmid#200396PurposeA plasmid IVT template containing a destabilized Firefly luciferase-PEST (degron) fusion followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.DepositorInsertFirefly luciferase - degron
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
EC71100
Plasmid#154068PurposeLevel 0 Golden Gate vector, pHvHSP17DepositorInsertpHvHSP17
UseStandard cloning vector used by geneart for synth…TagsExpressionMutationAll BsaI, Esp3I and BpiI restriction sites were r…PromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
EC71022
Plasmid#159608PurposeLevel 0.5 Golden Gate Vector, loxP-mCHERRY-HDEL-t35s-loxPDepositorInsertloxP-mCherry-HDEL-t35S-loxP
UseCre/Lox and Synthetic BiologyTagsExpressionMutationAll BsaI, Esp3I and BPiI restriction sites were r…PromoterAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJG85
Plasmid#89281PurposeEntry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sitesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEND-349_Cacnes_ori
Plasmid#225604PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector with C. acnes chloramphenicol resistance, harbouring a medium-copy pBR322 E. coli ori (no ROP)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
EC71171
Plasmid#154069PurposeLevel 0 Golden Gate vector, CRE with U5 intron at 254bp, in pICH41308 backboneDepositorInsertCRE_U5intron
UseSynthetic BiologyTagsExpressionMutationAll BsaI, Esp3I and BPiI restriction sites were r…PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
EC71139
Plasmid#154062PurposeLevel 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Barley Construct)DepositorInsertpZmUbi
UseSynthetic BiologyTagsExpressionMutationAll BsaI, Esp3I and BpiI restriction sites were r…PromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only