Addgene

We narrowed to 4,082 results for: Erf

Showing: 201 - 220 of 4082 results

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pCAG_mRuby2_smFP FLAG

Plasmid

#59760

Purpose

encodes multiple copies of an epitope tag FLAG ( not detected by anti GFP ab)

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

mRuby2_smFP_FLAG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG_mRuby2_smFP OLLAS

Plasmid

#59761

Purpose

encodes multiple copies of an epitope tag OLLAS ( not detected by anti GFP ab)

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

mRuby2_smFP_OLLAS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.GFPsm-myc.WPRE.SV40

Plasmid

#98926

Purpose

AAV expression of GFP spaghetti monster myc construct

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

GFP-smFP-myc

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CBA

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40

Plasmid

#98927

Purpose

Cre dependent AAV expression of GFP spaghetti monster myc construct

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Has Service

AAV1

Insert

GFP-smFP-myc

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Ruby2sm-Flag.WPRE.SV40

Plasmid

#98925

Purpose

AAV expression of Ruby2 spaghetti monster FLAG construct

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

Ruby2-smFP-Flag

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CBA

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pMRS-LacI-GFP

Plasmid

#220198

Purpose

mini-Tn7 GFP expression vector - LacI/pLac promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

monomeric superfolder green fluorescent protein

Use

CRISPR

Tags

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

LacI/pLac

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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SLC9A3R2_PDZ_2

Plasmid

#103926

Purpose

Protein expression and purification of NHERF2 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

SLC9A3R2 (NHERF2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMRS-BG42-GFP

Plasmid

#220202

Purpose

mini-Tn7 GFP expression vector - pBG42 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

monomeric superfolder green fluorescent protein

Use

CRISPR

Tags

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG42

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMRS-BG35-GFP

Plasmid

#220201

Purpose

mini-Tn7 GFP expression vector - pBG35 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

monomeric superfolder green fluorescent protein

Use

CRISPR

Tags

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG35

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMRS-AraC-GFP

Plasmid

#220199

Purpose

mini-Tn7 GFP expression vector - AraC/pBAD promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

monomeric superfolder green fluorescent protein

Use

CRISPR

Tags

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

AraC/pBAD

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRS-XylS-GFP

Plasmid

#220200

Purpose

mini-Tn7 GFP expression vector - XylS/pM promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

monomeric superfolder green fluorescent protein

Use

CRISPR

Tags

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

XylS/pM

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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MSGV1-1D3-28Z.1-3 mut

Plasmid

#107227

Purpose

Anti-mouse CD19 CAR with CD3 ITAMs mutated

Depositor

James Kochenderfer, Steven Rosenberg

Article

Kochenderfer et al Blood. 2010 Nov 11;116(19):3875-86. doi:

Insert

1D3-28Z. 1-3 mut

Use

Retroviral

Tags

None

Expression

Mutation

None

Promoter

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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MSGV-1D3-28Z All ITAMs intact

Plasmid

#107226

Purpose

Anti-mouse CD19 CAR with CD3 ITAMs intact

Depositor

James Kochenderfer, Steven Rosenberg

Article

Kochenderfer et al Blood. 2010 Nov 11;116(19):3875-86. doi:

Insert

1D3-28Z All ITAMs intact

Use

Retroviral

Tags

None

Expression

Mutation

None

Promoter

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40

Plasmid

#98928

Purpose

Cre dependent AAV expression of Ruby2 spaghetti monster FLAG construct

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Has Service

AAV1

Insert

Ruby2-smFP-Flag

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pFUGW hUbC-Sga-dCas9-KRAB-T2A-GFP

Plasmid

#227009

Purpose

Lentiviral Expression of dCas9-KRAB from S. gallolyticus fused to the repressive KRAB domain

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xFLAG, SV40 NLS

Expression

Mutation

Promoter

UbC

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pFUGW hUbC-Sin-dCas9-KRAB-T2A-GFP

Plasmid

#227010

Purpose

Lentiviral Expression of dCas9-KRAB from S. iniae fused to the repressive KRAB domain

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xFLAG, SV40 NLS

Expression

Mutation

Promoter

UbC

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pFUGW hUbC-Spa-dCas9-KRAB-T2A-GFP

Plasmid

#227011

Purpose

Lentiviral Expression of dCas9-KRAB from S. parasanguinis fused to the repressive KRAB domain

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xFLAG, SV40 NLS

Expression

Mutation

Promoter

UbC

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pFUGW hUbC-Sub-dCas9-KRAB-T2A-GFP

Plasmid

#227012

Purpose

Lentiviral Expression of dCas9-KRAB from S. uberis fused to the repressive KRAB domain

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xFLAG, SV40 NLS

Expression

Mutation

Promoter

UbC

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pMTgR2

Plasmid

#107286

Purpose

Plasmid can be used for Drosophila S2 driven secretion of extracellular part of human interferon-_ receptor 2.

Depositor

Bohdan Schneider

Article

Mikulecky et al Acta Crystallogr D Struct Biol. 2016 Sep

Insert

Interferon gamma receptor 2 (IFNGR2 Human)

Use

Tags

CATCATCACCATCACCATGA

Expression

Insect

Mutation

Promoter

Metallothionein promoter

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAAVS1-NC-CRISPRi (Gen3)

Plasmid

#73499

Purpose

Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

dCas9-KRAB

Use

CRISPR

Tags

HA, KRAB, and NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically deactivated Cas9)

Promoter

CAG

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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