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pME_Cp_0_1_004_psbA_5'Hom_Cr

Plasmid

#235785

Purpose

5' Homology psbA in the M1 position used for genome integration at the psbA locus

Depositor

Tobias Erb

Article

Inckemann et al bioRxiv 2024.05.08.593163

Insert

5' Homology region of the psbA locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_1_007_petB_5'Hom_cr

Plasmid

#235787

Purpose

5' Homology petB in the M1 position used for genome integration at the petB locus

Depositor

Tobias Erb

Article

Inckemann et al bioRxiv 2024.05.08.593163

Insert

5' Homology region of the petB locus of Chlamydomonas reinhardtii chloroplast CC-125

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His(6)-TEV-AtPCO4-2(wt)

Plasmid

#191802

Purpose

Full length wt AtPCO4-2 with Nt-His tag

Depositor

Emily Flashman

Article

White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

Insert

AtPCO4-2

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGWT35S-VC155

Plasmid

#194051

Purpose

Transient expression of VN155 (mVenus β-strands 8 to 11, amino acids 155−239) in plant cell (Cytosol)

Depositor

Yutaka Kodama

Article

Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

Insert

VC155 (mVenus β-strands 8 to 11, amino acids 155−239)

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pGWT35S-VN154

Plasmid

#194050

Purpose

Transient expression of VN154 (mVenus β-strands 1 to 7, amino acids 1−154) in plant cell (Cytosol)

Depositor

Yutaka Kodama

Article

Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

Insert

VN154 (mVenus β-strands 1 to 7, amino acids 1−154)

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His(6)-TEV-AtPCO5(wt)

Plasmid

#191803

Purpose

Full length wt AtPCO5 with Nt-His tag

Depositor

Emily Flashman

Article

White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

Insert

AtPCO5

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His(6)-AtPCO1(wt)

Plasmid

#191798

Purpose

Full length wt AtPCO1 with Nt-His tag

Depositor

Emily Flashman

Article

White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

Insert

AtPCO1

Tags

6xHis, Thrombin cleavage site

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His(6)-AtPCO2(wt)

Plasmid

#191799

Purpose

Full length wt AtPCO2 with Nt-His tag

Depositor

Emily Flashman

Article

White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

Insert

AtPCO2

Tags

6xHis, Thrombin cleavage site

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His(6)-AtPCO3(wt)

Plasmid

#191800

Purpose

Full length wt AtPCO3 with Nt-His tag

Depositor

Emily Flashman

Article

White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

Insert

AtPCO3

Tags

6xHis, Thrombin cleavage site

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSAC16

Plasmid

#137965

Purpose

Expresses Zea mays FNR and Prochlorococcus marinus NATL2A SIR

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Inserts

Zea mays Ferredoxin-NADP reductase
Procholoroccus marinus NATL2A sulfite reductase

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSAC17

Plasmid

#137966

Purpose

Expresses Zea mays FNR and Prochlorococcus marinus MIT9211 SIR

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Inserts

Zea mays Ferredoxin-NADP reductase
Procholoroccus marinus MIT9211 sulfite reductase

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b(+)-JHP933

Plasmid

#59302

Depositor

SeWon Suh

Article

Yoon et al Proteins. 2014 Mar 27. doi: 10.1002/prot

Insert

jhp0933

Tags

His tag

Expression

Bacterial

Mutation

aa1-232

Promoter

T7

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pVDL9.3

Plasmid

#168299

Purpose

Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

Depositor

Víctor de Lorenzo

Article

Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

Insert

Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD

Expression

Bacterial

Promoter

LacI-Plac

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pNOC-CRISPR-GFP

Plasmid

#100009

Purpose

Expresses Cas9-GFP and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into geneome

Depositor

Eva Farre

Article

Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

Type

Empty backbone

Use

Algae, nannochloropsis expression

Tags

Cas9-GFP

Promoter

Ribi promoter

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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mbYFPQS

Plasmid

#80742

Purpose

membrane-targeted biosensor for monitoring changes in intracellular chloride

Depositor

Susan Amara, Susan Ingram

Article

Watts et al PLoS One. 2012;7(4):e35373. doi: 10.1371

Insert

YFPQS

Tags

palmitoylation signal sequence of neuromodulin

Expression

Mammalian

Mutation

H148Q (CAC to CAG) and V163S (GTG to AGC).

Promoter

CMV

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only
pNOC-CRISPR-P2A-BlastR

Plasmid

#98147

Purpose

Expresses Cas9-Nlux-P2A-BlastR and sgRNA in Nannochloropsis oceanica.

Depositor

Eva Farre

Article

Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

Type

Empty backbone

Use

Algae, nannochloropsis expression

Tags

P2A-BlastR fusion with Cas9-Nlux

Promoter

Ribi promoter

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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csr-1_iRFP670_son-1_GpdToxA_bml_mTagBFP2

Plasmid

#191751

Purpose

Microtubule marker together with nuclear envelope marker driven by bidirectional promoter

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Inserts

NCU04288 (NCU04288 Neurospora crassa)
bml (Bml Neurospora crassa)

Tags

Gdp1 promoter, ToxA promoter, iRFP670, and mTagBF…

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMALp2x-SCO5461

Plasmid

#42507

Depositor

Koji Okamoto

Article

Nakano et al Toxicon. 2012 Dec 2;63C:55-63. doi: 10.1

Insert

guanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))

Tags

maltose-binding protein

Expression

Bacterial

Promoter

tac

Available Since

April 22, 2013

Availability

Academic Institutions and Nonprofits only
AAV-CAG-HyPerFLEX-NLSx3-WPRE

Plasmid

#217563

Purpose

Mammalian expression of HyPerFLEX targeted to the nucleus

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-NLS

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CaMKII-HyPerFLEX-UnaG-WPRE

Plasmid

#217564

Purpose

Expression of HyPerFLEX fused with UnaG in mammalian neurons

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-UnaG

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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