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We narrowed to 372 results for: P40

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MAPK1 gRNA (BRDN0001147498)

Plasmid

#77789

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK1 (MAPK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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shERK2a-mlpx-puro

Plasmid

#65229

Purpose

encodes a shRNA against ERK2

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

shRNA against ERK2 (MAPK1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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shERK1a-mlpx-puro

Plasmid

#65228

Purpose

encodes a shRNA against ERK1

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

shRNA against ERK2 (MAPK1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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shERK2b-mlpx-neo

Plasmid

#65231

Purpose

encodes a shRNA against ERK2

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

shRNA against ERK2 (MAPK1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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MK01_HUMAN_D0

Plasmid

#79713

Purpose

This plasmid encodes the kinase domain of MK01. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

Nicholas Levinson, John Chodera, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK01 (MAPK1 Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
CRaf-IRES-pErk reporter

Plasmid

#226170

Purpose

Used to transiently express Myc-tagged CRaf with the pErk reporter. Used in LABEL-seq MAPK activity assays.

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

Myc-CRaf-IRES-3xFlag-tdMCP-Erk2 (MAPK1, RAF1 Human)

Use

Tags

3xFlag-tdMCP and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pJK708

Plasmid

#71692

Purpose

Produces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Trp400>Phe mutant (AarAH6-W400F)

Depositor

T. J. Kappock

Insert

citrate synthase

Use

Tags

His6

Expression

Bacterial

Mutation

changed Trp400 to Phe

Promoter

T7 promoter

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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5538 pUG2-SSA1

Plasmid

#1247

Depositor

Susan Lindquist

Article

Glover et al Cell 1998 Jul 10;94(1):73-82.

Insert

Ssa1 (SSA1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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6377 H10-104b

Plasmid

#1248

Depositor

Susan Lindquist

Article

Glover et al Cell 1998 Jul 10;94(1):73-82.

Insert

Hsp104 (HSP104 Budding Yeast)

Use

Tags

his tag

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
6379 H10-K218T

Plasmid

#1250

Depositor

Susan Lindquist

Article

Glover et al Cell 1998 Jul 10;94(1):73-82.

Insert

Hsp104 (K218T) (HSP104 Budding Yeast)

Use

Tags

his tag

Expression

Bacterial

Mutation

K218T

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
6380 H10-K620T

Plasmid

#1251

Depositor

Susan Lindquist

Article

Glover et al Cell 1998 Jul 10;94(1):73-82.

Insert

Hsp104 (K620T) (HSP104 Budding Yeast)

Use

Tags

his tag

Expression

Bacterial

Mutation

K620T

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pNB649

Plasmid

#60136

Purpose

Firefly luciferase (Promega) driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Use

Tags

Expression

Yeast

Mutation

A4V

Promoter

MET17pr

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pNB769

Plasmid

#60138

Purpose

Red click beetle luciferase from Promega driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

CBR

Use

Tags

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pNB768

Plasmid

#60139

Purpose

Green click beetle luciferase from Promega driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

CBG99

Use

Tags

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-p47PX(aa2-132)

Plasmid

#19007

Depositor

Michael Yaffe

Article

Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

Insert

p47phox (NCF1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa2-132 (PX domains)

Promoter

Available since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
pNB778

Plasmid

#60154

Purpose

Green click beetle luciferase (Promega) driven by LEU1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

CBG99

Use

Tags

Expression

Yeast

Mutation

Promoter

LEU1pr

Available since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pNB648

Plasmid

#60140

Purpose

NanoLuc luciferase with a nuclear localization signal (NLS) driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

NLuc

Use

Tags

Nuclear localization signal (NLS)

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pNB783

Plasmid

#60162

Purpose

Firefly luciferase (Promega) driven by RNR1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Use

Tags

Expression

Yeast

Mutation

A4V & S504G (no functional effect)

Promoter

RNR1pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pNB777

Plasmid

#60153

Purpose

Red click beetle luciferase (Promega) driven by LEU1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

CBR

Use

Tags

Expression

Yeast

Mutation

Promoter

LEU1pr

Available since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pNB763

Plasmid

#60158

Purpose

Firefly luciferase (Promega) driven by LYS9 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Use

Tags

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

LYS9pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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