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Plasmid#165436PurposeExpresses ShadowY-CBD(H83L/H86L) and Clover(T153M/F223R)-Cdc42 in mammalian cells as a negative control for FLIM imaging.DepositorInsertShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-Cdc42
UseTagsShadowY, Clover(T153M/F223R)ExpressionMammalianMutationH83L/H86L: CDB(Cdc42 Binding Domain, Pak3, residu…PromoterCMVAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET_His-rsCaMPARI-mRuby3
Plasmid#120804PurposeExpresses rsCaMPARI in bacterial cellsDepositorInsertrsCaMPARI
UseTagsHis-Xpress and mRuby3ExpressionBacterialMutationPromoterT7Available sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn_NES-His-rsCaMPARI-mRuby3
Plasmid#120805PurposeAAV plasmid for expressing rsCaMPARI in neurons, nucleus excludedDepositorInsertrsCaMPARI
UseAAVTagsNES-His and mRuby3ExpressionMutationPromoterhsyn (synapsin-1)Available sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTol2-elavl3_NES-rsCaMPARI-mRuby3
Plasmid#122129PurposePan-neuronal expression of rsCaMPARI in zebrafish, nucleus excludedDepositorInsertrsCaMPARI
UseZebrafish expressionTagsNES-His and mRuby3ExpressionMutationPromoterelavl3Available sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn_NLS-His-rsCaMPARI-mRuby3
Plasmid#122092PurposeAAV plasmid for expressing rsCaMPARI in neurons, nucleus localizedDepositorInsertrsCaMPARI
UseAAVTagsNLS-His and mRuby3ExpressionMutationPromoterhsyn (synapsin-1)Available sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLC25A13-APEX2
Plasmid#228198Purposeplasmid encoding human SLC25A13 with an APEX protein tagDepositorInsertSLC25A13 (SLC25A13 Human)
UseSynthetic BiologyTagsAPEX2ExpressionMutationPromoterM13Available sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-HA-hTAF4b
Plasmid#17998DepositorInserthTAF4b (TAF4B Human)
UseTagsHAExpressionMammalianMutationaa1-64 deletedPromoterAvailable sinceMay 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
CFP-NS1
Plasmid#85739Purposeexpresses CFP-FLAG-tagged NS1 monobody.DepositorInsertFLAG-tagged NS1 monobody
UseTagsECFP and FlagExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGGG_Sr1644-1Sh
Plasmid#164087PurposeExpresses pGGG_Sr1644-1Sh in plantsDepositorInsertpGGG_Sr-1644-1Sh
UseTagsnoneExpressionBacterial and PlantMutationPromoterNative promoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSC38
Plasmid#104812PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr3g107390 (MtRdr6). Also expresses Cas9 from AtUBQ10 promoter.DepositorInsertMedtr3g107390
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC49
Plasmid#104823PurposeCRISPR/Cas9 2xplex gRNA targeting Glyma17g11235 (Dcl4a). Also expresses Cas9 from AtUBQ10 promoter.DepositorInsertGlyma17g11235
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC37
Plasmid#104811PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr3g107390 (MtRdr6). Also expresses Cas9 from Gmubi promoterDepositorInsertMedtr3g107390
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE myc-Raptor
Plasmid#18116DepositorInsertRaptor (RPTOR Human)
UseRetroviralTagsMycExpressionMammalianMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
myc-Raptor S722A/S792A
Plasmid#18118DepositorInsertRaptor (RPTOR Human)
UseTagsMycExpressionMammalianMutationS722A, S792APromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X2-RalBP1
Plasmid#37245DepositorInsertRalBP1 (RALBP1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRS712
Plasmid#213041PurposeCRISPR activation based overexpression in Nakaseomyces (Candida) glabrataDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBABE myc-Raptor S722A/S792A
Plasmid#18117DepositorInsertRaptor (RPTOR Human)
UseRetroviralTagsMycExpressionMammalianMutationS722A, S792APromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRS159
Plasmid#122378PurposeCRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS143
Plasmid#122377PurposeCRISPRi plasmid for use in Candida albicans. Contains dCas9 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLC-284
Plasmid#124294PurposeA low copy plasmid constitutively expressing vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.DepositorInsertvsfGFP-9
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only