We narrowed to 224 results for: lifeact

1. pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

   Plasmid

   #175526

   Purpose

   Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Lifeact-HaloTag11 and Tomm20-HaloTag7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag11 = HaloTag7-M175W

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTub-alpha1-EB3-NeonGreen-T2A-iLifeact-mCherry

   Plasmid

   #175261

   Purpose

   labelling of microtubule plus end and F-actin structures

   Depositor

   Frank Bradke, Sebastián Dupraz

   Article

   Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

   Insert

   EB3-NeonGreen-T2A-iLifeact-mCherry (MAPRE3 Human)
   

   Use

   Tags

   mCherry (on iLifeact) and mNeonGreen (on EB3)

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   pTub-alpha1

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Tet-Off-FLEX-Lifeact-mScarlet-HA-WPRE-pA (JDW 721)

   Plasmid

   #229826

   Purpose

   Tet-off, AAV vector for cre dependent expression of Lifeact mScarlet tagged with HA with EFS (minimal EF1a promoter or EF1a short/ EFS) driving destablized tTA (d2tTA)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mScarlet-I
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPI226

   Plasmid

   #113228

   Purpose

   safe haven knock-in plasmid for the act5 locus, Expression of LifeAct-mCherry

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Insert

   LifeAct
   

   Use

   Safe haven knock-in plasmid for the act5 locus, l…

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   act5

   Available since

   Aug. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPI227

   Plasmid

   #113231

   Purpose

   safe haven knock-in plasmid for the act5 locus, Expression of LifeAct-GFP

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Insert

   LifeAct
   

   Use

   Safe haven knock-in plasmid for the act5 locus,li…

   Tags

   GFP

   Expression

   Mutation

   Promoter

   act5

   Available since

   Aug. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGI3EM22C

   Plasmid

   #135488

   Purpose

   Agrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hph

   Depositor

   Nicolas Buchler

   Article

   Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

   Insert

   hph SpunH2A/Bpr LifeAct-tdTomato
   

   Use

   Tags

   LifeAct

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpunH2A/Bpr (divergent)

   Available since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. NK803

   Plasmid

   #166057

   Purpose

   Puromycin resistance and LifeAct-mCherry expression for Salpingoeca rosetta

   Depositor

   Nicole King

   Article

   Brunet et al Elife. 2021 Jan 15;10. pii: 61037. doi:

   Inserts

   Pac puromycin resistance gene
   LifeAct-mCherry
   

   Use

   S. rosetta expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMU-ACTLr

   Plasmid

   #61193

   Purpose

   Fluorescent reporter for F-actin LifeAct-mCherry expressed under AtUBQ10 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   LifeAct-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJP118

   Plasmid

   #176494

   Purpose

   Expresses Lifeact-mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoter

   Depositor

   Duojia Pan

   Article

   Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

   Insert

   Lifeact peptide
   

   Use

   Capsaspora owczarzaki expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMB-ACTLg

    Plasmid

    #61195

    Purpose

    Fluorescent reporter for F-actin LifeAct-eGFP expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    LifeAct-eGFP
    

    Use

    Tags

    eGFP

    Expression

    Plant

    Mutation

    Promoter

    MtBCP1

    Available since

    March 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMB-ACTLr

    Plasmid

    #61194

    Purpose

    Fluorescent reporter for F-actin LifeAct-mCherry expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    LifeAct-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    MtBCP1

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAV0782

    Plasmid

    #133514

    Purpose

    Expression of sfGFP tagged LifeAct probe to monitor actin cytoskeleton

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pAde6(PmeI)-p(act1)-LifeAct-sfGFP-terminator(ScADH1)-bsdMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAV0783

    Plasmid

    #133515

    Purpose

    Expression of mCherry tagged LifeAct probe to monitor actin cytoskeleton

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pAde6(PmeI)-p(act1)-LifeAct-mCherry-terminator(ScADH1)-bsdMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPI138

    Plasmid

    #113229

    Purpose

    extrachromosomal expression vector, expression of LifeAct-GFP

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Insert

    LifeAct
    

    Use

    Dictyostelium discoideum expression vector, extra…

    Tags

    GFP

    Expression

    Mutation

    Promoter

    act15

    Available since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPI161

    Plasmid

    #207439

    Purpose

    Shuttle vector LifeAct-mCherry

    Depositor

    Rob Kay

    Article

    Nichols et al Dev Cell. 2019 Feb 25;48(4):491-505.e9.

    Insert

    LifeAct-mCherry
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPI304

    Plasmid

    #113232

    Purpose

    extrachromosomal expression vector, expression of LifeAct-mCherry/PH-pkgE-GFP

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Insert

    LifeAct & pkgE-PH
    

    Use

    Dictyostelium discoideum expression vector, extra…

    Tags

    mCherry & GFP

    Expression

    Mutation

    Promoter

    act15

    Available since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)

    Plasmid

    #99229

    Purpose

    Mammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEos2
    

    Use

    Tags

    Lifeact

    Expression

    Mammalian

    Mutation

    A69T for primed conversion applications

    Promoter

    CMV

    Available since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. NK612

    Plasmid

    #109092

    Purpose

    Reporter gene expression in S. rosetta

    Depositor

    Nicole King

    Article

    Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

    Insert

    Lifeact-mCherry
    

    Use

    Choanoflagellate expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSP64-GFP-ePDZ-CAAX

    Plasmid

    #135603

    Purpose

    Expression of GFP-ePDZ-CAAX mRNA for microinjection to be used for optogenetics in S. purpuratus

    Depositor

    Mamiko Yajima

    Article

    Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

    Insert

    Lifeact-GFP-ePDZ-CAAX
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    CAAX, EGFP, and ePDZ

    Expression

    Mutation

    Promoter

    Unknown

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNP151

    Plasmid

    #106359

    Purpose

    To insert a single copy of a construct at a site on Chr II, corresponding to a mKate-labelled LifeAct expressed specifically in the adult epidermis.

    Depositor

    Jonathan Ewbank

    Article

    Taffoni et al Elife. 2020 Jan 29;9. pii: 45047. doi: 1

    Insert

    col-62p_lifeact_mkate2_3'nmy-2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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