We narrowed to 1,145 results for: ttl

Showing: 201 - 220 of 1145 results

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pUPD2 PhiC31 RL (attR:P35S:TMtb:attL) (GB1506)

Plasmid

#160582

Purpose

35S promoter inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attR and attL)

Depositor

Diego Orzaez

Publication

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

attR:P35S:TMtb:attL

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pMIRNA1-METTL1-msg6-EIR107-109AAA

Plasmid

#254423

Purpose

Mammalian expression plasmid encoding mutant human METTL1 (sgMETTL1-6-resistant) fused to a 3_FLAG tag.

Depositor

Rui Su

Publication

Ren et al Blood. 2026 Jun 18;147(25):3069-3085. do

Insert

METTL1 (METTL1 Human)

Use

Lentiviral

Tags

3_FLAG

Mutation

EIR107-109AAA

Promoter

CMV

Available since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pMIRNA1-METTL1-msg3-EIR107-109AAA

Plasmid

#254422

Purpose

Mammalian expression plasmid encoding mutant human METTL1 (sgMETTL1-3-resistant) fused to a 3_FLAG tag.

Depositor

Rui Su

Publication

Ren et al Blood. 2026 Jun 18;147(25):3069-3085. do

Insert

METTL1 (METTL1 Human)

Use

Lentiviral

Tags

3_FLAG

Mutation

EIR107-109AAA

Promoter

CMV

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop Mettl3 HDR

Plasmid

#207136

Purpose

DNA sequence source for amplifying an HDR template for mouse Mettl3 knockout and reporter knock-in

Depositor

Silvia Monticelli

Publication

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

5'HDR Mettl3 - ZsGreen- sv40(polyA) - 3'HDR Mettl3 (Mettl3 Mouse)

Expression

Mammalian

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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M3-M14-dCas9

Plasmid

#134779

Purpose

Expression of fusion protein M3-M14-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Publication

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Insert

METTL3/METTL14 methyltransferase domain and inactive dCas9 (METTL3, METTL14 Human, S. pyogenes)

Expression

Mammalian

Promoter

CMV

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas13-inactive M3M14nes

Plasmid

#157855

Purpose

Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

Depositor

David Liu

Publication

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Insert

dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)

Expression

Mammalian

Mutation

PspCas13b Δ984-1090 H133A; METTL3 D395A

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas13-inactive M3nls

Plasmid

#157854

Purpose

Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

Depositor

David Liu

Publication

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Insert

dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)

Expression

Mammalian

Mutation

PspCas13b Δ984-1090 H133A; METTL3 D395A

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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SPDK3876(TRV-RNA2-pPEBV-MCS)

Plasmid

#149275

Purpose

Modified Tobacco rattle virus RNA2 vector that could be used for expression of proteins or RNAs

Depositor

Savithramma Dinesh-Kumar

Publication

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

Modified TRV RNA2 with PEBV promoter

Use

T-dna vector

Mutation

T2318C (DNA) in PEBV coat protein sequence

Available since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHel2

Plasmid

#102960

Purpose

E.coli/H.Pylori shuttle vector

Depositor

Rainer Haas

Publication

Heuermann et al Mol Gen Genet. 1998 Mar;257(5):519-28.

Type

Empty backbone

Use

Plasmid shuttle vector system for genetic complem…

Available since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHel3

Plasmid

#102961

Purpose

E.coli/H.Pylori shuttle vector

Depositor

Rainer Haas

Publication

Heuermann et al Mol Gen Genet. 1998 Mar;257(5):519-28.

Type

Empty backbone

Use

Shuttle vector

Available since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSF463

Plasmid

#167671

Purpose

Intermediate vector for two gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSF464

Plasmid

#167672

Purpose

Intermediate vector for four gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pG106 - KA

Plasmid

#178041

Purpose

Bacteroides - Escherichia coli shuttle vector with ErmF and ErmAM, Kan and Amp selection markers

Depositor

Janina Lewis

Publication

Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

Type

Empty backbone

Use

Shuttle vector

Expression

Bacterial

Available since

Feb. 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pKO403_lacZ_Cm

Plasmid

#174725

Purpose

Bifidobacterium-E.coli shuttle vector for seamless gene cloning

Depositor

Tohru Suzuki

Publication

Altaib et al Microbiol Resour Announc. 2022 Jan 20;11

Type

Empty backbone

Expression

Bacterial

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pSF278

Plasmid

#167673

Purpose

Intermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSII415

Plasmid

#35454

Depositor

Steven Haase

Publication

Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

Type

Empty backbone

Use

Yeast centromere vector

Promoter

None

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSII416

Plasmid

#35456

Depositor

Steven Haase

Publication

Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

Type

Empty backbone

Use

Yeast centromere vector

Promoter

None

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pYUB28b

Plasmid

#37277

Depositor

Ted Baker

Publication

Bashiri et al PLoS One. 2010 Dec 29;5(12):e15803.

Type

Empty backbone

Use

Mycobacteria shuttle vector

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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M3D395A-M14-dCas9

Plasmid

#134780

Purpose

Expression of fusion protein M3D395A-M14-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Publication

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Insert

METTL3/METTL14 methyltransferase domain and inactive dCas9

Expression

Mammalian

Mutation

D395A

Promoter

CMV

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB

Plasmid

#52889

Purpose

"CoinFLP-Gal4" - Expresses Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express Gal4, or FRT3-FRT3 pair to prevent Gal4 expression

Depositor

Iswar Hariharan

Publication

Bosch et al Development. 2015 Feb 1;142(3):597-606.

Insert

Gal4 (GAL4 Budding Yeast)

Expression

Insect

Available since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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