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Showing: 5451 - 5500 of 38145 results

  11. pORTMAGE311B

    Plasmid
    #120418
    Purpose
    Expresses Lambda Beta and a dominant negative MutL E32K allele, controlled by XylS-Pm expression system for high precision and efficient MAGE experiments at 37°C. RSF1010 Ori; Broad host-range; KanR
    Depositor
    Csaba Pál
    Article
    Szili et al bioRxiv (2018)
    Inserts
    MutL E32K
    Lambda Beta
    XylS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E32K mutation conferring dominant mutator phenoty…
    Promoter
    Pm
    Available since
    Feb. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    120418-plasmid-map-sequence-id-236013

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  17. pX037

    Plasmid
    #167156
    Purpose
    MoClo-compatible Level2 vector for autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, nnH3H, nnLuz and nnCPH. Does not contain NpgA.
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    caffeic acid cycle genes
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    pNOS, p35s
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167156-plasmid-map-sequence-id-335288

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  18. pAGT9569

    Plasmid
    #219431
    Purpose
    Transcriptional unit (MoClo-position 2): pAtRPS5a_PapE-4LF2-N-SpCas9i-N_tNOS
    Depositor
    Tom Schreiber
    Article
    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000
    Insert
    LB_AtRPS5a:PapE-4xLF2-NLS-SpCas9i-NLS:tNOS_RB (Position 2)
    Use
    CRISPR and Synthetic Biology; Moclo compatible le…
    Tags
    PapE-4LF2
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    219431-plasmid-map-sequence-id-436703

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  19. pUltra-Exon1Q23-Myc-B

    Plasmid
    #110488
    Purpose
    Lentiviral vector to express wild-type N terminal HTT gene with partial long 3'UTR in mammalian cells (encodes Myc-tagged wild-type huntingtin exon1 fragment with 23 repeats of Q)
    Depositor
    Baoji Xu
    Article
    Xu et al PLoS One. 2017 May 11;12(5):e0177610. do
    Insert
    Homo sapiens huntingtin (HTT Human)
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    short 3'UTR mutant huntingtin exon1 fragment…
    Promoter
    UbC
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    110488-plasmid-map-sequence-id-212609

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  25. Cav2.1 EA2, R57A, R59A pMT2

    Plasmid
    #206122
    Purpose
    expression of rabbit Cav2.1 calcium channel with a CaV2.1-P1217fs mutation causing a truncated protein, (EA2). R57A R59A mutations prevent dominant-negative suppression of Cav2.1 currents
    Depositor
    Annette Dolphin
    Article
    Dahimene et al Neurobiol Dis. 2016 Sep;93:243-56. doi:
    Insert
    cacna1a (Cacna1a Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Cav2.1-P1217fs, R57A, R59A
    Promoter
    Ad MLP/TPL/SV40
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    206122-plasmid-map-sequence-id-415911

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  29. pLPC-L647RLaminA-CS

    Plasmid
    #69062
    Purpose
    encodes a lamin A mutant with Leucine 647 mutated to an Arginin and with a substitution of cysteine in CaaX-motif by a serine
    Depositor
    Gerardo Ferbeyre
    Article
    Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d
    Insert
    LaminA (LMNA Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Lamin A with Leucine 647 mutated to Arginine and …
    Promoter
    CMV
    Available since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only

  32. pAED2

    Plasmid
    #234599
    Purpose
    Fluorescent reporter of the SOS response
    Depositor
    Tanel Tenson
    Article
    Kaldalu et al NPJ Antimicrob Resist. 2025 Apr 11;3(1):
    Inserts
    mScarlet-I
    gfp-mut2
    Use
    Reporter
    Tags
    Expression
    Bacterial
    Mutation
    GFPmut2 was derived from avGFP with the following…
    Promoter
    Ptet+dnaK P1 and cda
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234599-plasmid-map-sequence-id-467741

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  33. pCKC775

    Plasmid
    #226626
    Purpose
    piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT Human, S. pyogenes)
    EF1a promoter, sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    7xTCF-LEF and EF1a
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226626-plasmid-map-sequence-id-449161

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  34. pCKC764

    Plasmid
    #226625
    Purpose
    piggyBAC pCAG-hCas9-T2A-Unbiased3
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT Human, S. pyogenes, Chicken)
    EF1a promoter, blasticidin resistance
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and EF1a
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226625-plasmid-map-sequence-id-449160

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  35. pCKC776

    Plasmid
    #226627
    Purpose
    piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT Human, S. pyogenes)
    EF1a promoter, mCherry
    Use
    Synthetic Biology
    Tags
    oxygen dependent degron (ODD)
    Expression
    Mammalian
    Mutation
    Promoter
    4xHRE_YB TATA and EF1a
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226627-plasmid-map-sequence-id-449157

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  40. pLPC-S22AProgerin-CS

    Plasmid
    #69063
    Purpose
    encodes a Progerin mutant with serine 22 mutated to an alanine and with a substitution of cysteine in CaaX-motif by a serine
    Depositor
    Gerardo Ferbeyre
    Article
    Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d
    Insert
    Progerin (LMNA Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Progerin with serine 22 mutated to alanine and wi…
    Promoter
    CMV
    Available since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only

  44. pHEE401E

    Plasmid
    #71287
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    gRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71287-plasmid-map-sequence-id-352460

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  46. pFUSE2ss-CLIg-mK_M18

    Plasmid
    #82358
    Purpose
    Expression plasmid coding for kappa light chain of mouse antibody specific to antigen B of the ABO blood group system, clone M18.
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103
    Insert
    mouse immunoglobulin kappa light chain, variable fragment derived from clone M18 (Igk, Igkc, Igk-V Mouse)
    Use
    Tags
    none
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1-HTLV prom
    Available since
    Sept. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    82358-plasmid-map-sequence-id-400982

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  47. pAGT9054

    Plasmid
    #219430
    Purpose
    Transcriptional unit (MoClo-position 2): 2x35Ss_Omega enhancer_PapE-4LF2-N-SpCas9i-N_tOCS
    Depositor
    Tom Schreiber
    Article
    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000
    Insert
    LB_2x35Ss-omega enhancer:PapE-4xLF2-NLS-SpCas9i-NLS:tOCS_RB (Position 2)
    Use
    CRISPR; Moclo compatible level 1 module (position…
    Tags
    PapE-4LF2
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219430-plasmid-map-sequence-id-441037

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  49. pHEE401

    Plasmid
    #71286
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…
    Available since
    Jan. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    71286-plasmid-map-sequence-id-127777

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  50. pMDC32B-AtTAS1c-D2-B/c-AtMIR173

    Plasmid
    #137885
    Purpose
    Plant expression vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor downstream 3'D1[+]. Contains AtMIR173 for syntasi expression in any plant species.
    Depositor
    Alberto Carbonell
    Article
    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583
    Inserts
    AtTAS1c-D2-B/c
    2x35S-AtMIR173-Tnos
    Use
    Tags
    Expression
    Plant
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Promoter
    Available since
    May 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    137885-plasmid-map-sequence-id-275856

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Showing: 5451 - 5500 of 38145 results