We narrowed to 12,157 results for: pcDNA
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Plasmid#191848Purposeexpress C57D/C146D mutant SOD1 in mammalian cellsDepositorAvailable SinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only
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pcDNA3.1-MS2-HA-HsNot4_377-428_AG
Plasmid#148757PurposeMammalian Expression of HsNot4_377-428DepositorInsertHsNot4_377-428 (CNOT4 Human)
ExpressionMammalianAvailable SinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsRck_296-472_AC
Plasmid#148419PurposeMammalian Expression of HsRck_296-472DepositorInsertHsRck_296-472 (DDX6 Human)
ExpressionMammalianAvailable SinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PARL-D319N-FLAG C-t
Plasmid#191957PurposeExpression of Presenilin Associated Rhomboid Like (PARL) D319N mutantDepositorAvailable SinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-DEST47-Noa1-GFP
Plasmid#191968PurposeGateway Destination Vector, containing NOA1-GFP chimera coding sequence, for Gateway Cloning TechnologyDepositorInsertNoa1-GFP
UseSynthetic BiologyAvailable SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Cep41-STf
Plasmid#182010PurposeCMV overexpression of SNAP-tag fast in mammalian cell lines associated to microtubulesDepositorInsertCep41-STf
ExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_H2B-STf
Plasmid#181998PurposeCMV overexpression of SNAP-tag fast fusion to H2B for nuclear localization in mammalian cell linesDepositorInsertH2B-STf
ExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_b4g-STf
Plasmid#182005PurposeCMV overexpression of SNAP-tag fast in the golgi apparatus of mammalian cell linesDepositorInsertb4g-STf
ExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HNF1B-FUS-IDR
Plasmid#183240PurposeExpression of HNF1B fused to a intrinsically disordered regions of the FUS proteinDepositorInsertHNF1 homeobox B (HNF1B Human)
TagsFusion to an IDR from the FUS protein and FLAG ta…ExpressionMammalianPromoterCMVAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HNF1A-delta-IDR2
Plasmid#183238PurposeExpression of FLAG tagged HNF1A with deletion of a intrinsically disordered regions (AA545-573)DepositorInsertHNF1 homeobox A (HNF1A Human)
TagsFLAG tagExpressionMammalianMutationDeletion of amino acids 545-573PromoterCMVAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1(+)-PKAcat(G1A)-mCherry
Plasmid#181852PurposemCherry-tagged PKA catalytic subunit lacking N-terminal myristoylation site; for mammalian expression.DepositorInsertPKAcat-mCherry (PRKACA Human)
TagsmCherryExpressionMammalianMutationGlycine residue immediately after start site in P…PromoterCMVAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-NLS-YFP-CCTc
Plasmid#184327PurposeMammalian expression of the nuclear localized YFP-CCTd(peptide of Cav1.2)DepositorInsertCCTc (CACNA1C Human)
ExpressionMammalianAvailable SinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-AKAR (mScarlet-I)
Plasmid#181861PurposeFluoSTEP-AKAR using mScarlet-I as the FRET acceptor.DepositorInsertFluoSTEP-AKAR (mScarlet-I)
TagsmScarlet-I and sfGFP(1-10)ExpressionMammalianPromoterCMVAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-hAP1 420-440
Plasmid#183643PurposeTo (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expressionDepositorInsertATP6AP1 (ATP6AP1 Human)
TagsmycExpressionMammalianMutationtruncated ATP6AP1 mutant; contains only the trans…PromoterCMVAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Prom1 Del EC2
Plasmid#184025PurposeMammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 273-287.DepositorInsertProm1 SV8(-Ex19a) Del EC2 (Prom1 Mouse)
TagsFlagExpressionMammalianMutationDeletion of exon 19a (amino acids 696-701), and d…Available SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Prom1 SV8 (-Ex19a)
Plasmid#184012PurposeMammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701).DepositorInsertProm1 SV8(-Ex19a) (Prom1 Mouse)
TagsFlagExpressionMammalianMutationDeletion of exon 19a (amino acids 696-701).Available SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA T7-Gnat1 Mut-3UTR
Plasmid#184027PurposeExpresses mouse Gnat1 protein with N-terminal T7 tag from cDNA that contains the mutant 3'-UTR where the TAG binding sites for MSI1 are mutated to TGADepositorInsertGnat1 (Gnat1 Mouse)
TagsT7ExpressionMammalianMutationTAG sites in the 3'-UTR are mutated to TGAPromoterCMVAvailable SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Hygro(+)_GRHL1-R9A-L3F6H
Plasmid#177767PurposeOverexpression of 3xFLAG-6xHis tagged human GRHL1 (R9A mutation)DepositorAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Hygro(+)_GRHL1-S95A-L3F6H
Plasmid#177768PurposeOverexpression of 3xFLAG-6xHis tagged human GRHL1 (S95A mutation)DepositorAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Hygro(+)_GRHL1-K116A-L3F6H
Plasmid#177769PurposeOverexpression of 3xFLAG-6xHis tagged human GRHL1 (K116A mutation)DepositorAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Hygro(+)_GRHL1-T139A-L3F6H
Plasmid#177770PurposeOverexpression of 3xFLAG-6xHis tagged human GRHL1 (T139A mutation)DepositorAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Hygro(+)_GRHL1-K370A-L3F6H
Plasmid#177771PurposeOverexpression of 3xFLAG-6xHis tagged human GRHL1 (K370A mutation)DepositorAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Hygro(+)_GRHL1-S374A-L3F6H
Plasmid#177772PurposeOverexpression of 3xFLAG-6xHis tagged human GRHL1 (S374A mutation)DepositorAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Hygro(+)_GRHL1-K592A-L3F6H
Plasmid#177773PurposeOverexpression of 3xFLAG-6xHis tagged human GRHL1 (K592A mutation)DepositorAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsSmg5-G120E_W
Plasmid#147819PurposeMammalian Expression of HsSmg5-G120EDepositorInsertHsSmg5-G120E (SMG5 Human)
ExpressionMammalianMutationone silent mutation compared to the sequence give…Available SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsSmg7_633-1091_T
Plasmid#147558PurposeMammalian Expression of HsSmg7_633-1091DepositorInsertHsSmg7_633-1091 (SMG7 Human)
ExpressionMammalianMutationone non silent mutation V854I compared to the seq…Available SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-TRBP-dsRBD2
Plasmid#180602PurposeExpressing various parts of TRBPDepositorAvailable SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-TRBP-dsRBD1
Plasmid#180601PurposeExpressing various parts of TRBPDepositorAvailable SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-α-COPI-WD40-Arg57Ala
Plasmid#180759PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg57Ala (residues 1-327)DepositorInsertα-COPI-WD40-Arg57Ala
TagsC-terminal strep tagExpressionMammalianMutationEncodes residues 1-327; mutations: Arg57Ala, Leu1…PromoterCMVAvailable SinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-α-COPI-WD40-Tyr139Ala
Plasmid#180761PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Tyr139Ala (residues 1-327)DepositorInsertα-COPI-WD40-Tyr139Ala
TagsC-terminal strep tagExpressionMammalianMutationEncodes residues 1-327; mutations: Tyr139Ala, Leu…PromoterCMVAvailable SinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-α-COPI-WD40-Asp115Ala
Plasmid#180760PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Asp115Ala (residues 1-327)DepositorInsertα-COPI-WD40-Asp115Ala
TagsC-terminal strep tagExpressionMammalianMutationEncodes residues 1-327; mutations: Asp115Ala, Leu…PromoterCMVAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TRBP-L2+D2+L3-myc/His
Plasmid#180600PurposeExpressing various parts of TRBPDepositorAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-gp130dimer-stm2585
Plasmid#174395PurposeConstitutively active gp130 dimer with codon-optimized stm2585 insert (replacing amino acids 140-179)DepositorInsertIL6ST cytosolic domain and sarA chimera
ExpressionMammalianAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-stm2585-gp130
Plasmid#174393PurposeChimera of codon-optimized stm2585 (sarA/steE) and human gp130 sequence.DepositorInsertsarA-IL6ST chimera
TagsFlagExpressionMammalianMutation140-177 of sarA replaced with homologous IL6ST se…Available SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3HA-AMOT p130∆PPxY2
Plasmid#166712Purposeexpresses AMOT with inactivating mutation in second PPxY motif in mammalian cellsDepositorInsertAngiomotin p130 P262A P263A (AMOT Human)
Tags2xHAExpressionMammalianMutationchanged proline 262 to alanine, proline 263 to al…PromoterCMV promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3HA-AMOT p130∆PPxY3
Plasmid#166713Purposeexpresses AMOT with inactivating mutation in third PPxY motif in mammalian cellsDepositorInsertAngiomotin p130 P3072A P308A (AMOT Human)
Tags2xHAExpressionMammalianMutationchanged proline 307 to alanine, proline 308 to al…PromoterCMV promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mas-PRGRH-Nluc
Plasmid#158608PurposePlasmid expressing PRG(RH)-Nluc in mammalian cellsDepositorInsertmyristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - PDZ RhoGEF RH domain - linker (GGTGGS) - Nluc
ExpressionMammalianPromoterCMVAvailable SinceJuly 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-mas-p115RH-Nluc
Plasmid#158607PurposePlasmid expressing p115(RH)-Nluc in mammalian cellsDepositorInsertmyristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - p115RhoGEF RH domain - linker (GGTGGS) - Nluc
ExpressionMammalianPromoterCMVAvailable SinceJune 29, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDNA3_PKA-CαN (E12,14A)-mVenus
Plasmid#168499PurposeMammalian expression of the E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
ExpressionMammalianAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (K8,9R)-mVenus
Plasmid#168498PurposeMammalian expression of the K8,9R mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe K8,9R mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
ExpressionMammalianAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN(K8,9A)-mPAGFP
Plasmid#168494PurposeMammalian expression of the K8,9A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertK8,9A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.
ExpressionMammalianAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (E12,14K)-mVenus
Plasmid#168488PurposeMammalian expression of the E12,14K mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe E12,14K mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
ExpressionMammalianAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (K8,9E)-mVenus
Plasmid#168487PurposeMammalian expression of the E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
ExpressionMammalianAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (K22,24A)-mVenus
Plasmid#168484PurposeMammalian expression of the K22,24A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe K22,24A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
ExpressionMammalianAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (K8,9A)-mVenus
Plasmid#168483PurposeMammalian expression of the K8,9A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe K8,9A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
ExpressionMammalianAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (G2A)-mVenus
Plasmid#168482PurposeMammalian expression of the G2A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe G2A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
ExpressionMammalianAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3. Flag-CSL Q307R
Plasmid#167009PurposeMammalian expression of CSL Q307RDepositorAvailable SinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3. Flag-CSL A258R
Plasmid#167008PurposeMammalian expression of CSL A258RDepositorAvailable SinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3. Flag-CSL V237A
Plasmid#167007PurposeMammalian expression of CSL V237ADepositorAvailable SinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only