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pLenti-PGK-RAP2A-V12-neo

Plasmid

#156178

Purpose

Lentiviral vector for expression of RAP2A-V12, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Expression

Mammalian

Mutation

changed Glycine 12 to Valine

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-flag-RAP1B-V12-neo

Plasmid

#156175

Purpose

Lentiviral vector for expression of flag-RAP1B-V12, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1B (RAP1B Bovine)

Use

Lentiviral

Tags

2xFLAG

Expression

Mammalian

Mutation

changed Glycine 12 to Valine

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-flag-RAP1B-N17-neo

Plasmid

#156176

Purpose

Lentiviral vector for expression of flag-RAP1B-N17, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1B (RAP1B Bovine)

Use

Lentiviral

Tags

2xFLAG

Expression

Mammalian

Mutation

changed Serine 17 to Asparagine

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pGL4.23 C3_25

Plasmid

#60305

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ROCK1 enhancer (ROCK1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pLenti-PGK-RAP2A-N17-neo

Plasmid

#156179

Purpose

Lentiviral vector for expression of RAP2A-N17, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Expression

Mammalian

Mutation

changed Serine 17 to Asparagine

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.23 C3_9

Plasmid

#60295

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

NKX6-1 enhancer (NKX6-1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pLentiCMVblast_SLC38A2_N82A

Plasmid

#156181

Purpose

CMV-driven expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.

Depositor

Alec Kimmelman

Article

Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

Insert

SLC38A2 (SLC38A2 Human)

Expression

Mammalian

Mutation

Silent mutations to prevent targeting by sgRNA: T…

Promoter

CMV

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pInducer20_SLC38A2_N82A

Plasmid

#156183

Purpose

Dox-inducible expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.

Depositor

Alec Kimmelman

Article

Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

Insert

SLC38A2 (SLC38A2 Human)

Expression

Mammalian

Mutation

Silent mutations to prevent targeting by sgRNA: T…

Promoter

TRE

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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JDW 822 (pDONR221-V5-mScarlet-I-KRAS4A-WT)

Plasmid

#156405

Purpose

Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4A-WT (KRAS Human)

Use

Gateway cloning

Tags

mScarlet-I

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.23 C3_29

Plasmid

#60309

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ZFP36L2 enhancer (ZFP36L2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pENTR D-TOPO-C3_10

Plasmid

#60259

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

RFX6 enhancer (RFX6 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_25

Plasmid

#60268

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ROCK1 enhancer (ROCK1 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Ai14-luc

Plasmid

#87118

Purpose

AAV backbone plasmid including luc-pA knock-in donor and Ai14gRNA for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

U6-Ai14sgRNA-Luciferase-nEF-GFPKASH-hGHpA

Use

AAV, CRISPR, and Luciferase

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23 C3_31

Plasmid

#60310

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

SLC2A2 enhancer (SLC2A2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.23 C3_21

Plasmid

#60296

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

CDKN2BAS enhancer (CDKN2B-AS1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.23 C3_19

Plasmid

#60302

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

THADA enhancer (THADA Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
TDP-43_CTD_12S→E_S305C/A326P

Plasmid

#248868

Purpose

Expresses MBP-tagged C-terminal domain of TDP-43 with 12S→E, S305C and A326P.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_12S→E_S305C/A326P (TARDBP Human)

Tags

6xHis tags-MBP

Expression

Bacterial

Mutation

changed S292E, S369E, S377E, S379E, S387E, S389E,…

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_13S→E_S317C

Plasmid

#248845

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 13S→E and S317C.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_13S→E_S317C (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed S292E, S305E, S369E, S377E, S379E, S387E,…

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TDP-43_CTD_13S→E_S273C

Plasmid

#248859

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 13S→E and S273C.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_13S→E_S273C (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed S292E, S305E, S369E, S377E, S379E, S387E,…

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TDP-43_CTD_12S→E_S305C

Plasmid

#248867

Purpose

Expresses MBP-tagged C-terminal domain of TDP-43 with 12S→E and S305C.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_12S→E_S305C (TARDBP Human)

Tags

6xHis tags-MBP

Expression

Bacterial

Mutation

changed S292E, S369E, S377E, S379E, S387E, S389E,…

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge