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Showing: 2181 - 2200 of 2316 results

  1. pBABE-MCS(extended)-Puro (empty vector)

    Plasmid
    #172603
    Purpose
    Empty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.
    Depositor
    Michele Pagano
    Article
    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    The multiple cloning site (MCS) has been edited/e…
    Promoter
    Available since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172603-plasmid-map-sequence-id-340216

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  2. pTight-9-124-BclxL

    Plasmid
    #60857
    Purpose
    2nd generation lentiviral transfer plasmid. Expresses a synthetic cluster of miR-9/9* and miR-124 fused to Bcl-xL under a doxycycline-inducible promoter
    Depositor
    Andrew Yoo
    Article
    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1
    Insert
    miR-9/9*-miR-124-BCLxL (BCL2L1 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pTight
    Available since
    Dec. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    60857-plasmid-map-sequence-id-195309

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  5. pGEX6P1‐hsRNASEH2BCA

    Plasmid
    #108692
    Purpose
    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzyme
    Depositor
    Andrew Jackson, Martin Reijns
    Article
    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9
    Inserts
    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A (RNASEH2A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Shine Dalgarno sequence upstream of start codon a…
    Promoter
    tac
    Available since
    April 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    108692-plasmid-map-sequence-id-208976

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  8. G9A[SET]-dCas9

    Plasmid
    #100089
    Purpose
    Catalytic domain [SET] of human G9A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Insert
    G9A (EHMT2 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-G9A[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    G9A[SET] includes aa 829-1209
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100089-plasmid-map-sequence-id-197561

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  10. pGEX6P1‐hsRNASEH2BCA(D34A/D169A)

    Plasmid
    #108693
    Purpose
    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A (with D34A and D169A catalytic site mutations) for purification of the RNase H2 trimeric enzyme
    Depositor
    Andrew Jackson, Martin Reijns
    Article
    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9
    Inserts
    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A-D34A/D169A
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Shine Dalgarno sequence upstream of start codon, …
    Promoter
    tac
    Available since
    April 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    108693-plasmid-map-sequence-id-208987

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  12. pMDC32B-AtTAS1c-D2-B/c-AtMIR173

    Plasmid
    #137885
    Purpose
    Plant expression vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor downstream 3'D1[+]. Contains AtMIR173 for syntasi expression in any plant species.
    Depositor
    Alberto Carbonell
    Article
    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583
    Inserts
    AtTAS1c-D2-B/c
    2x35S-AtMIR173-Tnos
    Use
    Tags
    Expression
    Plant
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Promoter
    Available since
    May 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    137885-plasmid-map-sequence-id-275856

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  14. PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-ins

    Plasmid
    #78099
    Purpose
    Nuclear fluorescent reporter in mammalian cells, phiC31 attB for integration, can be silenced with TetR-CR or rTetR-CR (e.g. CR can be KRAB or DNMT3B).
    Depositor
    Michael Elowitz
    Article
    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do
    Insert
    Histone H2B - Citrine (H2BC21 Synthetic, Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    5xTetO pEF alpha
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only

  16. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899,884 (All)

    Plasmid
    #194190
    Purpose
    Includes the promoter (1kb) of SMTS with 5 mutated KLF binding sites (positions 449,548,732,899,884)
    Depositor
    Ralf Brandes, Matthias Leisegang
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    5 mutated KLF binding sites (positions 449,548,73…
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194190-plasmid-map-sequence-id-381947

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  17. pGL3-pSMANTIS_1kb_KLF2mut 899

    Plasmid
    #194193
    Purpose
    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 899)
    Depositor
    Ralf Brandes, Matthias Leisegang
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated KLF binding sites (position 899)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194193-plasmid-map-sequence-id-381931

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  19. pCRISPR-Cas9/CD4-TK2

    Plasmid
    #104397
    Purpose
    The designed sgRNA cloned into this plasmid directs the specific DNA cleavage exerted by Cas9 nuclease in a region of exon 5 of the human TK2 gene. The plasmid also includes the Cas9 nuclease and CD4.
    Depositor
    Jeronimo Blanco
    Article
    Meca-Cortes et al Mol Ther Nucleic Acids. 2017 Sep 15;8:39
    Insert
    TK2 (TK2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    104397-plasmid-map-sequence-id-208338

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Showing: 2181 - 2200 of 2316 results