166,034 results

1. 6xHis-MBP-huMb2Cas12a

   Plasmid

   #174676

   Purpose

   Expresses 6xHis-MBP tagged Mb2Cas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huMb2Cas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF0675

   Plasmid

   #141659

   Purpose

   Lentiviral vector for overexpressing the PRDM16 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PRDM16 (PRDM16 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-BAZ1A

   Plasmid

   #65371

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BAZ1A (BAZ1A Human)
   

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   796I compared to all reference sequences

   Promoter

   CMV

   Available Since

   June 15, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pLV-EF1a-H2B-miRFP670

   Plasmid

   #212668

   Purpose

   Express tagged H2B

   Depositor

   Yumi Konagaya, Tobias Meyer

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   H2B (H2BC11 Human)
   

   Use

   Lentiviral

   Tags

   miRFP670

   Promoter

   EF-1a

   Available Since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3.1(+)-NEMOm

   Plasmid

   #189930

   Purpose

   Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

   Depositor

   Yubin Zhou

   Article

   Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

   Insert

   NEMOs
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. mTFP1-pBAD

   Plasmid

   #54553

   Purpose

   Localization: Protein Expression Vector , Excitation: 462, Emission: 492

   Depositor

   Robert Campbell, Michael Davidson

   Article

   Ai et al Biochem J. 2006 Dec 15. 400(3):531-40.

   Type

   Empty backbone

   Tags

   6xHis and mTFP1

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUW

   Plasmid

   #14882

   Depositor

   David Baltimore

   Article

   Lois et al Science. 2002 Feb 1. 295(5556):868-72.

   Insert

   flap-Ub promoter-WRE
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 25, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223_TNFRSF17_WT

   Plasmid

   #82263

   Purpose

   Gateway Donor vector containing TNFRSF17 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   TNFRSF17 (TNFRSF17 Human)
   

   Use

   Gateway entry vector

   Mutation

   N81S; K86E

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPP381

   Plasmid

   #158798

   Purpose

   pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   dCasPhi-2
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   RuvC-inactivating mutation D394A

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS-SKII-3XHA-fCLIP-NAT

    Plasmid

    #188921

    Purpose

    Integration cassette to create C-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    3XHA-fCLIP-NAT
    

    Expression

    Yeast

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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