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pIRESneo-FLAG/HA-Ago2-Y529E

Plasmid

#215842

Purpose

Expression of AGO2-Y529E mutant

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

Human AGO2 (AGO2 Human)

Expression

Mammalian

Mutation

Ago2-Y529E

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-IFT43

Plasmid

#218735

Purpose

Expresses N-terminally EGFP-tagged IFT43 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

Insert

IFT43 (IFT43 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-Universal.Sth.dCas9

Plasmid

#171088

Purpose

CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.

Depositor

Sebastian Lourido

Article

Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

Inserts

Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
DHFR-TSc3

Use

Toxoplasma gondii expression

Mutation

Note: A S245F mutation is present but did not app…

Promoter

DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pClneoFLAG human Dendrin

Plasmid

#47934

Purpose

expresses FLAG tagged human Dendrin in mammalian cells

Depositor

Yutaka Hata

Article

Kawata et al J Biochem. 2006 May;139(5):931-9.

Insert

Dendrin-1 (DDN Human)

Tags

FLAG

Expression

Mammalian

Mutation

contains amino acids 2-711 of human Dendrin

Promoter

CMV

Available Since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only
GW1-mito-mCherry-roGFP2

Plasmid

#107220

Purpose

Mammalian expression plasmid to target the mCherry-roGFP2 sensor to the mitochondrial matrix.

Depositor

Mathew Tantama

Article

Norcross et al ACS Sens. 2017 Nov 22;2(11):1721-1729. d

Insert

mCherry-roGFP2

Tags

4xCox8

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pJZC620

Plasmid

#62282

Purpose

Expresses dCas9, MCP-VP64, and PCP-VP64 in Yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

MCP-VP64
PCP-VP64
dCas9

Tags

VP64

Expression

Yeast

Mutation

Nuclease activity has been inactivated by mutatio…

Promoter

pAdh and pTdh3

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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ELF3_pet28a

Plasmid

#131658

Purpose

Bacterial expression of ETS gene ELF3

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELF3 (ELF3 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Venus-MDGA2

Plasmid

#82544

Purpose

Overexpression vector for Venus-MDGA2 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

MDGA2 (Mdga2 Rat)

Tags

Venus fluorescent protein

Expression

Mammalian

Promoter

chicken beta actin

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K3-2xCSTREP

Plasmid

#136150

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K3

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K3

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_HCLS1-1/1

Plasmid

#91437

Purpose

Protein expression and purification of human SH3 domain construct HCLS1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

HCLS1-1/1 (HCLS1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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