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pLenti-CaMKIIa-eNpHR-EYFP-WPRE

Plasmid

#20946

Purpose

3rd gen lentiviral expression of halorhodopsin 2.0 (eNpHR) fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

halorhodopsin

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Promoter

CaMKIIa

Available Since

May 29, 2009

Availability

Academic Institutions and Nonprofits only

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pQCXIP-HA.Tetherin

Plasmid

#41069

Depositor

Paul Spearman

Article

Hammonds et al PLoS Pathog. 2010 Feb 5;6(2):e1000749.

Insert

Tetherin (BST2 Human)

Use

Retroviral

Tags

HA and IRES Puro

Expression

Mammalian

Promoter

CMV/MSV

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only
pBlu/gRNA

Plasmid

#59188

Purpose

Serves as a shuttle vector for target oligo before insertion into Cas9 destination vector

Depositor

Robert Stupar

Article

Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

Insert

Guide RNA Cassette

Use

CRISPR

Expression

Plant

Promoter

U6 (Arabidopis)

Available Since

Feb. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCIS GEM-CEPIA1er

Plasmid

#58217

Purpose

Ratiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

GEM-CEPIA1er

Tags

myc

Expression

Mammalian

Mutation

GEM-GECO1 E298D F359W D400E E407D

Promoter

CAG

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-myrSNAP

Plasmid

#60098

Purpose

Expresses membrane-targeted SNAP(f)-tag and histone-GFP, non-conditional

Depositor

Gregory Jefferis

Article

Kohl et al Proc Natl Acad Sci U S A. 2014 Aug 25. p

Inserts

SNAP-tag
histone-GFP

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

March 26, 2015

Availability

Academic Institutions and Nonprofits only

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pETM6-Amp-T7-roGFP

Plasmid

#202464

Purpose

Expresses redox sensitive GFP (roGFP) with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

reduction-oxidation sensitive green fluorescent protein

Tags

6X-His

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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scAAV-hSyn-Cre

Plasmid

#138253

Purpose

A self-complementary AAV vector expresses Cre recombinase from hSyn promoter.

Depositor

Li Zhang

Article

Zingg et al J Neurosci. 2020 Apr 15;40(16):3250-3267

Insert

Cre recombinase

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

hSyn

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pET29a-YS14

Plasmid

#66890

Purpose

Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)

Depositor

Jung-Hyun Min

Article

Shim et al Anal Biochem. 2012 Aug 15;427(2):190-2.

Inserts

His6-Thrombin-Xenopus laevis histones H2A
Xenopus laevis histones H2B
Xenopus laevis histones H3
Xenopus laevis histones H4-Thrombin

Tags

H2A, H2B, H3, H4, His6 tag, and Thrombin

Expression

Bacterial

Promoter

T7

Available Since

Sept. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Plasmid

#163059

Purpose

mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

mito-roGFP2-Orp1

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-Lime

Plasmid

#191255

Purpose

Bacterial expression plasmids for pH sensitive fluorescent protein Lime

Depositor

Robert Campbell

Article

Shen et al ACS Sens. 2023 Aug 25;8(8):3014-3022. do

Insert

Lime

Tags

His tag

Expression

Bacterial

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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