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pET-Cas9-NLS-6xHis

Plasmid

#62933

Purpose

Expression of Cas9-NLS-6xHis in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

Cas9-NLS-6xHis

Tags

6xHis tag and NLS

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-TFE3

Plasmid

#38120

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFE3 (TFE3 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-ATF6

Plasmid

#32955

Purpose

For visualization of GFP-ATF6 in mammalian cells.

Depositor

Ron Prywes

Article

Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

Insert

ATF6 (ATF6 Human)

Tags

EGFP

Expression

Mammalian

Mutation

M67L and M313I

Promoter

CMV

Available Since

Nov. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pMLM290

Plasmid

#21872

Depositor

Keith Joung

Article

Foley et al Nat Protoc. 2009. 4(12):1855-67.

Type

Empty backbone

Use

Zebrafish expression

Tags

Flag

Expression

Mammalian

Available Since

Nov. 3, 2009

Availability

Academic Institutions and Nonprofits only

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Flag Snail WT

Plasmid

#16218

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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Stat3 Flag pRc/CMV

Plasmid

#8707

Depositor

Jim Darnell

Article

Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.

Insert

Stat3 (Stat3 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

*see comments

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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APEX2-NLS

Plasmid

#124617

Purpose

proximity biotinylation

Depositor

Alice Ting

Article

Kaewsapsak et al Elife. 2017 Dec 14;6. doi: 10.7554/eLife

Insert

APEX2-NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCRIS-PITChv2-BSD-dTAG (BRD4)

Plasmid

#91792

Purpose

PITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

BSD-P2A-2xHA-FKBP_F36V

Use

CRISPR

Expression

Mammalian

Mutation

Phenylalanine 36 to valine in FKBP12

Promoter

Promoterless

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-3XMyc-EGFP-OMP25

Plasmid

#83355

Purpose

For tagging mitochondria with Myc epitopes (Control-MITO construct)

Depositor

David Sabatini

Article

Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

Insert

3XMyc-EGFP-OMP25

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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paavCAG-post-mGRASP-2A-dTomato

Plasmid

#34912

Purpose

post

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Has Service

AAV5

Insert

postsynaptic mGRASP

Use

AAV

Promoter

CAG

Available Since

Nov. 25, 2013

Availability

Academic Institutions and Nonprofits only

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