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Plasmid#198690PurposePeriplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody H4 (AviTag) in BL21(DE3)DepositorInsertanti-SARS-CoV-2 Nucleocapsid nanobody H4
UseTags6xHis tag, Avitag, OmpA signal peptide, and Ribos…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLC047 - pL2_p2x35s-CrGLK-tMAS
Plasmid#203906PurposeOverexpression of the CrGLK coding sequence from C. roseus in a plant binary vector for Agrobacterium-mediated transformation.DepositorInsertCrGLK
UseTagsExpressionPlantMutationSilent mutation of BpiI or BsaI cut site for MoCl…PromoterCaMV 2x35SAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMDC32-FIT2-N[80]A
Plasmid#96992PurposeExpress mouse FIT2 gene in plants.DepositorInsertMmFIT2 (Fitm2 Mouse)
UseTagsExpressionPlantMutationAmino acid residue 80 was mutated (N[80]A). Mutat…Promoter35SAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9Pubi-H
Plasmid#66187Purposeexpression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selectionDepositorInsertCas9
UseTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotermaize ubiquitin promoter (Pubi)Available sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9Pubi-B
Plasmid#66188Purposeexpression of Cas9 in plants, maize ubiquitin promoter (Pubi), basta selectionDepositorInsertCas9
UseTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotermaize ubiquitin promoter (Pubi)Available sinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEAT8-137 M1V
Plasmid#173807PurposeExpression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cellsDepositorInsertSERPiNA1 (SERPINA1 Human)
UseTagsExpressionBacterialMutationE1M Otherwise this plasmid encodes the most commo…PromoterT7Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9pUbi-N
Plasmid#106331PurposeExpression of Cas9 in plants, ZmUbi promoter, Kanamycin selectionDepositorInsertCas9
UseCRISPRTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…PromoterZmUbiAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGB2Ω2_SF-35s:hCas9:tNos (GB1103)
Plasmid#75400PurposeTranscriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter. Specially conceived to be linked with the TU of the kanamycin resistance gene GB1181.DepositorInserthCas9
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoter35SAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_CH3-1
Plasmid#105859PurposeExpression plasmid coding for hybrid heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG1-derived CH3.DepositorUseTagsExpressionMammalianMutationhybrid heavy chain of mouse IgG3 with CH3 derived…PromoterhEF1-HTLV promAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_CH2-1
Plasmid#105857PurposeExpression plasmid coding for hybrid heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG1-derived CH2.DepositorUseTagsExpressionMammalianMutationhybrid heavy chain of mouse IgG3 with CH2 derived…PromoterhEF1-HTLV promAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_CH1h-1
Plasmid#105856PurposeExpression plasmid coding for hybrid heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG1-derived CH1 and hinge.DepositorUseTagsExpressionMammalianMutationhybrid heavy chain of mouse IgG3 with CH1 and hin…PromoterhEF1-HTLV promAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_h-1
Plasmid#105861PurposeExpression plasmid coding for hybrid heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG1-derived hinge.DepositorUseTagsExpressionMammalianMutationhybrid heavy chain of mouse IgG3 with hinge deriv…PromoterhEF1-HTLV promAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1-NbON10-NanoLuc
Plasmid#198691PurposeExpresses anti-SARS-Cov-2 Nucleocapsid nanobody ON10 fused to NanoLuc in HEK293 cell cultureDepositorInsertanti-SARS-CoV-2 Nucleocapsid nanobody ON10
UseTags6xHis tag, IgK leader sequence, NanoLuc, and twin…ExpressionMammalianMutationPromoterCMV promoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET303C-hSOD1 A4V/C6S
Plasmid#139667PurposeExpression plasmids containing human A4V/C6S SOD1DepositorInsertSuperoxide dismutase-1 (SOD1 Human)
UseTagsExpressionBacterialMutationchange alanine 4 to valine, change cysteine 6 to …PromoterT7Available sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_VPEVS
Plasmid#105863PurposeExpression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody has mutated 5 amino acids in lower hinge.DepositorInsertIgG3 heavy chain (Ighg3 Mouse)
UseTagsExpressionMammalianMutationmutated heavy chain of mouse IgG3 with five mutat…PromoterhEF1-HTLV promAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG1_ILGGP
Plasmid#105855PurposeExpression plasmid coding for mutated heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody has mutated 5 amino acids in lower hinge.DepositorInsertIgG1 heavy chain (Ighg1 Mouse)
UseTagsExpressionMammalianMutationmutated heavy chain of mouse IgG1 with five mutat…PromoterhEF1-HTLV promAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMDC43-FIT2-N[80]A
Plasmid#96995PurposeExpress GFP-tagged mutated mouse FIT2 gene in plants.DepositorInsertMmFIT2 (Fitm2 Mouse)
UseTagsGFPExpressionPlantMutationAmino acid residue 80 was mutated (N[80]A). Mutat…Promoter35SAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBABEneo mt T Ag
Plasmid#133713PurposeLarge T antigen mutant form in pBABEneo vector for mammalian expression.DepositorInsertSV40gp6 (SV40gp6 SV40)
UseRetroviralTagsExpressionMutationaa 1-354 of T antigen (including aa 1-130); no p5…PromoterAvailable sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9P35S-H
Plasmid#66189Purposeexpression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), hygro selectionDepositorInsertCas9
UseTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotercauliflower mosaic virus 35S promoter (P35S)Available sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9P35S-N
Plasmid#66191Purposeexpression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selectionDepositorInsertCas9
UseTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotercauliflower mosaic virus 35S promoter (P35S)Available sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only