Addgene

We narrowed to 6,346 results for: Rel

Showing: 2201 - 2220 of 6346 results

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pBSV2_Psyn-sgRNA500

Plasmid

#149613

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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PHY23-phage-ubc-nls-ha-stdMCP-tdTomato

Plasmid

#164044

Purpose

Expression of stdMCP-tdTomato in mammalian cells

Depositor

Baohui Chen

Article

Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

Insert

stdMCP-tdTomato

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

human ubiquitin C (UBC)

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-FLAG-R-Ras2

Plasmid

#102335

Purpose

Human R-Ras2 with FLAG tag for mammalian expression

Depositor

Hening Lin

Article

Zhang et al Elife. 2017 Apr 13;6. pii: e25158. doi:

Insert

RRAS2 (RRAS2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pclbw-mitoCFP

Plasmid

#58426

Purpose

Mammalian expression of CFP targeted to the mitochondrial matrix

Depositor

David Chan

Article

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

mito-CFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

cmv-bactin

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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Atg24 1B

Plasmid

#197046

Purpose

His6 tagged Atg24 from S. cerevisiae for expression in E. coli.

Depositor

Michael Ragusa

Article

Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/

Insert

Atg24

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBSV2_P0026-mCherryBb

Plasmid

#118239

Purpose

Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0026 promoter driven mCherry

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

bb0026 promoter driven mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pDR FLIP43 GPS1

Plasmid

#101084

Purpose

GA biosensor expression plasmid

Depositor

Alexander Jones, Wolf Frommer

Article

Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

Insert

GPS1

Use

Tags

6HIS and cMyc

Expression

Yeast

Mutation

Promoter

pPMA1

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2 Notch1 ICv-6MT

Plasmid

#41730

Depositor

Raphael Kopan

Article

Schroeter et al Nature. 1998 May 28;393(6683):382-6.

Insert

Notch-1 Intracellular Domain (Notch1 Mouse)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

Contains murine Notch-1 protein beginning at Vali…

Promoter

Available since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pAD6

Plasmid

#134349

Purpose

E. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tag

Depositor

Seth Darst

Article

Deaconescu et al Acta Crystallogr Sect F Struct Biol Crys

Insert

mfd (mfd E. coli)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-mOrange

Plasmid

#214807

Purpose

Mammalian expression vector

Depositor

Norio Komatsu

Article

De Marchi et al Leuk Res. 2022 Aug;119:106883. doi: 10.1

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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PKC beta II CAT

Plasmid

#16384

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta II (Prkcb Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

CAT: catalytic domain, aa 329-673

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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mpeg:Rab7-stop-polyA; cmlc2:GFP

Plasmid

#213738

Purpose

Expresses mCherry-Rab7 in the macrophage lineage of zebrafish with a green heart transgenesis marker

Depositor

William Talbot

Article

Iyer et al Sci Adv. 2022 Sep 2;8(35):eabp8321. doi:

Insert

mCherry-Rab7

Use

Zebrafish

Tags

Expression

Mutation

Promoter

mpeg1.1

Available since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-SNAP-CLIP-GB1a

Plasmid

#125709

Purpose

Eukaryotic expression of a fusion protein encoding SNAP, CLIP and the GB1a subunit of the metabotropic GABA receptor

Depositor

Kai Johnsson

Article

Masharina et al J Am Chem Soc. 2012 Nov 21;134(46):19026

Insert

SNAP-CLIP-GB1a

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pGL-FLKif3B

Plasmid

#13743

Depositor

Linda Wordeman

Article

Ginkel et al Mol Biol Cell. 2000 Dec . 11(12):4143-58

Insert

mouse Kif3B (Kif3b Mouse)

Use

Cre/Lox

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2007

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-msfYFPBb

Plasmid

#118233

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfYFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven msfYFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pminiTol2-HuC-NE2h

Plasmid

#208689

Purpose

Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h pan-neuronally in zebrafish

Depositor

Yulong Li

Insert

GPCR activation based norepinephrine (NE) sensor GRAB_NE2h

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Huc

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pKIKan_idCas9_Chr_center

Plasmid

#149637

Purpose

suicide vector for knock in into the B. burgdorferi chromosome

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-mCeruleanBb

Plasmid

#118235

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCerulean

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven mCerulean

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_speG_Cterm_His

Plasmid

#220483

Purpose

heterologous expression for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

speG

Use

Tags

6xHs

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLNC Wnt-3a

Plasmid

#17994

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-3a (Wnt3a Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only