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Showing: 5501 - 5550 of 32140 results

  6. pNSU299_MET16

    Plasmid
    #166091
    Purpose
    Plasmid for constituive spCas9 and tet-inducible MET16 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    MET16 (MET16 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    May 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166091-plasmid-map-sequence-id-329609

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  7. pMCSG53_Nsp3_NAB_2

    Plasmid
    #167260
    Purpose
    Bacterial expression of Nsp3 Nucleic acid binding domain (residues 1089-1203)
    Depositor
    Alexei Savchenko
    Insert
    Nsp3 (residues 1089-1203) (ORF1ab SARS-CoV-2)
    Use
    Tags
    His6-TEV site
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Available since
    April 27, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167260-plasmid-map-sequence-id-331337

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  8. pMCSG53_Nsp3_NAB_1

    Plasmid
    #167259
    Purpose
    Bacterial expression of Nsp3 Nucleic acid binding domain (residues 1050-1216)
    Depositor
    Alexei Savchenko
    Insert
    Nsp3 (residues 1050-1216) (ORF1ab SARS-CoV-2)
    Use
    Tags
    His6-TEV site
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Available since
    April 27, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167259-plasmid-map-sequence-id-331336

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  9. pANY4-pL18-ccdB

    Plasmid
    #164898
    Purpose
    Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    164898-plasmid-map-sequence-id-327989

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  10. pNSU299_CPR1_1

    Plasmid
    #166074
    Purpose
    Plasmid for constituive spCas9 and tet-inducible CPR1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    CPR1 (CPR1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166074-plasmid-map-sequence-id-330357

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  11. pNSU299_CPR1_2

    Plasmid
    #166071
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of an sgRNA targeting an intergenic site near CPR1 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Intergenic region near CPR1
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166071-plasmid-map-sequence-id-330355

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  12. pNSU299_GAL7

    Plasmid
    #166089
    Purpose
    Plasmid for constituive spCas9 and tet-inducible GAL7 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    GAL7 (GAL7 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166089-plasmid-map-sequence-id-330353

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  13. pNSU299_CUP1-1

    Plasmid
    #166086
    Purpose
    Plasmid for constituive spCas9 and tet-inducible CUP1-1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    CUP1-1 (CUP1-1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166086-plasmid-map-sequence-id-330352

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  14. pNSU299_GLK1

    Plasmid
    #166090
    Purpose
    Plasmid for constituive spCas9 and tet-inducible GLK1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    GLK1 (GLK1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166090-plasmid-map-sequence-id-329612

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  15. pNSU299_LHS1

    Plasmid
    #166092
    Purpose
    Plasmid for constituive spCas9 and tet-inducible LHS1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    LHS1 (LHS1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166092-plasmid-map-sequence-id-329611

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  16. pNSU299_APL3

    Plasmid
    #166073
    Purpose
    Plasmid for constituive spCas9 and tet-inducible APL1-targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    APL3 (APL3 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166073-plasmid-map-sequence-id-329133

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  17. pNSU299_YBL055C

    Plasmid
    #166077
    Purpose
    Plasmid for constituive spCas9 and tet-inducible YBL055C targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    YBL055C (near PTC3) (YBL055C Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166077-plasmid-map-sequence-id-329132

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  18. pNSU299_SLA1

    Plasmid
    #166075
    Purpose
    Plasmid for constituive spCas9 and tet-inducible SLA1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    SLA1 (SLA1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166075-plasmid-map-sequence-id-329130

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  19. pNSU299_PPE1

    Plasmid
    #166083
    Purpose
    Plasmid for constitutive spCas9 and tet-inducible PPE1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    PPE1 (PPE1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166083-plasmid-map-sequence-id-329122

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  20. pNSU299_PDR12

    Plasmid
    #166093
    Purpose
    Plasmid for constituive spCas9 and tet-inducible PDR12 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    PDR12 (PDR12 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166093-plasmid-map-sequence-id-329121

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  21. pNSU299_TDH2

    Plasmid
    #166082
    Purpose
    Plasmid for constituive spCas9 and tet-inducible TDH2 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    TDH2 (TDH2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166082-plasmid-map-sequence-id-329117

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  22. pNSU299_LYS9

    Plasmid
    #166085
    Purpose
    Plasmid for constituive spCas9 and tet-inducible LYS9 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    LYS9 (LYS9 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166085-plasmid-map-sequence-id-329116

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  23. pNSU299_GAL2

    Plasmid
    #166088
    Purpose
    Plasmid for constituive spCas9 and tet-inducible GAL2 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    GAL2 (GAL2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166088-plasmid-map-sequence-id-329112

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  24. pCASCADE-udhA-zwf

    Plasmid
    #87153
    Purpose
    udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    udhA-zwf guide array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87153-plasmid-map-sequence-id-174933

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  25. tdTomato-Talin-ABM-10

    Plasmid
    #58133
    Purpose
    Localization: Talin Actin Binding Domain, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    Talin ABM
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Actin Binding Module; I/LWEQ = 197 amino acids fr…
    Promoter
    CMV
    Available since
    Dec. 18, 2014
    Availability
    Academic Institutions and Nonprofits only

  26. mCitrine-Talin-ABM-10

    Plasmid
    #56569
    Purpose
    Localization: Talin Actin Binding Domain, Excitation: 516, Emission: 529
    Depositor
    Michael Davidson
    Insert
    Talin ABM
    Use
    Tags
    mCitrine
    Expression
    Mammalian
    Mutation
    Actin Binding Module; I/LWEQ = 197 amino acids fr…
    Promoter
    CMV
    Available since
    Dec. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  27. mOrange2-Talin-ABM-10

    Plasmid
    #57972
    Purpose
    Localization: Talin Actin Binding Domain, Excitation: 549, Emission: 565
    Depositor
    Michael Davidson
    Insert
    Talin ABM
    Use
    Tags
    mOrange2
    Expression
    Mammalian
    Mutation
    Actin Binding Module; I/LWEQ = 197 amino acids fr…
    Promoter
    CMV
    Available since
    Oct. 24, 2014
    Availability
    Academic Institutions and Nonprofits only

  28. pSB-Bleo Gal4 CCS1

    Plasmid
    #139189
    Purpose
    SB-transposon with constitutive bleomycin resistance and Gal4-inducible coffee caffeine synthase 1 (CCS1).
    Depositor
    Neal Devaraj
    Insert
    caffeine synthase 1 [Coffea arabica]
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Gal4-inducible CMV and STAT3
    Available since
    Availability
    Academic Institutions and Nonprofits only
    139189-plasmid-map-sequence-id-309396

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  31. pLVX ALIX-GFP WT

    Plasmid
    #138582
    Purpose
    Expresses fluorescent ALIX in mammalian cells
    Depositor
    Wesley Sundquist
    Insert
    ALIX (PDCD6IP Human)
    Use
    Lentiviral
    Tags
    GFP and h30
    Expression
    Mammalian
    Mutation
    Promoter
    TREtight
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    138582-plasmid-map-sequence-id-275088

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  32. p2B-58

    Plasmid
    #177928
    Purpose
    Expresses yeGFP under control of doxycycline-responsive TET4 promoter (contains four rtTA binding sites)
    Depositor
    Vadim Gladyshev
    Article
    Barre et al (unpublished)
    Insert
    EGFP
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    TET4 (contains four rtTA binding sites)
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177928-plasmid-map-sequence-id-349996

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  34. pCW57-MCS1-P2A-MCS2 (Neo)

    Plasmid
    #89180
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.
    Depositor
    Adam Karpf
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89180-plasmid-map-sequence-id-176308

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  35. pCW57.1 CMYC

    Plasmid
    #164145
    Purpose
    Doxycycline inducible expression of c-MYC
    Depositor
    Richard Possemato
    Insert
    MYC (MYC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Doxycycline inducible promoter
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    164145-plasmid-map-sequence-id-325859

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  36. pAAV-synp-F-synaptophysin-GCaMP6f

    Plasmid
    #102996
    Purpose
    Expresses synaptophysin fused GCaMP6f under the synapsin promoter
    Depositor
    David Cox
    Insert
    Synaptophysin-GCaMP6f
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    102996-plasmid-map-sequence-id-218069

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  40. pcDNA3-HA-CIRBP

    Plasmid
    #11607
    Depositor
    Steven Johnson
    Insert
    Cold Inducible RNA Binding Protein (CIRBP Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    no mutations
    Promoter
    Available since
    July 18, 2007
    Availability
    Academic Institutions and Nonprofits only

  41. pCT02

    Plasmid
    #212152
    Purpose
    Plasmid expressing 6x His tagged FAST-PETase under inducible promoter PT7 control.
    Depositor
    Kang Zhou
    Insert
    FAST-PETase
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    S121E, D186H, R224Q, N233K, R280A
    Promoter
    PT7
    Available since
    March 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    212152-plasmid-map-sequence-id-424774

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  48. pBV514

    Plasmid
    #204602
    Purpose
    Expresses F74A OsTir1-3X Myc from S. cerevisiae ADH1 promoter
    Depositor
    Scott Moye-Rowley
    Insert
    Os Tir1 (LOC4335696 Oryza sativa)
    Use
    Tags
    Myc epitope tags
    Expression
    Bacterial
    Mutation
    Changed phenylalanine 74 into an alanine (F74A).
    Promoter
    ScADH1
    Available since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    204602-plasmid-map-sequence-id-417406

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Showing: 5501 - 5550 of 32140 results