We narrowed to 5,822 results for: pCas

1. pCAG-Flag-Avi-ires-CHD5

   Plasmid

   #68878

   Purpose

   Transient mammalian expression of CHD5 for BirA tagging

   Depositor

   Adrian Bracken

   Article

   Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

   Insert

   CHD5 (CHD5 Human)
   

   Tags

   FLAG/AVI

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pspCas9-ABE-3'UTR-sgRNA-2xMS2

   Plasmid

   #132554

   Purpose

   Vector plasmid expressing ABEmax and sgRNA scaffold with MS2 replacing the Tetraloop and loop 2

   Depositor

   Baisong Lu

   Article

   Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

   Inserts

   ABEmax
   sgRNA, with Tetraloop and loop 2 replaced by MS2 aptamer
   

   Expression

   Mammalian

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3xNLS-NLP-cMyc-cMyc enAspCas12a

   Plasmid

   #182127

   Purpose

   pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   3xNLS-NLP-cMyc-cMyc enAspCas12a
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCALNL-eGFP-NT1-6-gix-6-NT1

   Plasmid

   #81204

   Purpose

   contains two target sites consisting of PAM-NT1-6bp-gix psuedo site-6bp-NT1-PAM flanking a kan/pA as previously described in the pCALNL-eGFP plasmid

   Depositor

   David Liu

   Article

   Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

   Insert

   gix-Neo-gix deletion cassette upstream of EGFP
   

   Expression

   Mammalian

   Promoter

   pCBa

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-mcherry-WIPI2d-cs-TEV-STREP

   Plasmid

   #178912

   Purpose

   Mammalian expression of human WIPI2d with N-terminal mCherry and C-terminal Strep

   Depositor

   James Hurley

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2d
   

   Tags

   C term strep and N term mcherry

   Expression

   Mammalian

   Available Since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3xNLS-NLP-cMyc-cMyc AspCas12a

   Plasmid

   #182122

   Purpose

   pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   3xNLS-NLP-cMyc-cMyc AspCas12a
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS/Daxx myc S502A/669A

   Plasmid

   #19283

   Depositor

   Philip Leder

   Article

   Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

   Insert

   Daxx (Daxx Mouse)
   

   Expression

   Mammalian

   Available Since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

8. -161+80pCalb2/E2F2/NRF-1(-64)-like

   Plasmid

   #66742

   Purpose

   luciferase reporter for Calb2 promoter (-161to +80, mutant)

   Depositor

   Rolf Stahel, Emanuela Felley-Bosco

   Article

   Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

   Insert

   Calb2 promoter (-161bp+80) (CALB2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   CGCG(-64) to TACA

   Promoter

   Calb2

   Available Since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-EGFP/RFP-miR-E1-1int

   Plasmid

   #19819

   Depositor

   Zuoshang Xu

   Article

   Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

   Insert

   miRNA
   

   Use

   Cre/Lox and RNAi

   Expression

   Mammalian

   Available Since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pCAG-Venus-pA_U6-BbsI (pE057)

    Plasmid

    #149351

    Purpose

    Mammalian expression vector for Venus

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    Venus
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. M11 Cardiac Actin Promoter pCarA

    Plasmid

    #17148

    Depositor

    Randall Moon

    Insert

    cardiac actin promoter (actc1 Frog)
    

    Use

    Xenopus expression

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pCAG-BFP-pA_U6-BbsI (pE058)

    Plasmid

    #149352

    Purpose

    Mammalian expression vector for BFP

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    BFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCSDest2-SpCas9-NLS-3XHA-NLS

    Plasmid

    #69220

    Purpose

    Expresses wild type SpCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-Flag-wtSpCas9 (without sgRNA)

    Plasmid

    #92353

    Purpose

    Expression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFlag-NLS-wild-type SpCas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBT250_(pCA-G-intron(Neo)-tTA2)

    Plasmid

    #36876

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tTA2
    beta-globin intron
    Neo
    

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-sfGFP-GSAx9-iCre-ERT2

    Plasmid

    #125748

    Purpose

    sfGFP and iCreERT2 fused by GSAx9 linker

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    sfGFP-GSAx9-iCre-ERT2
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBT241_(pCA-ATG-intron-tTA2)

    Plasmid

    #36875

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Insert

    tTA2
    

    Expression

    Mammalian

    Mutation

    insertion of a beta-globin intron with a loxP sit…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer)

    Available Since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAA)

    Plasmid

    #137859

    Purpose

    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAA N-terminus)

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-LplA(AILR,36)-NRX3b D137A

    Plasmid

    #43920

    Depositor

    Alice Ting

    Article

    Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

    Insert

    AILR-LplA36-NRX3b (lplA Human, Synthetic)
    

    Tags

    2XHA

    Expression

    Mammalian

    Mutation

    D137A

    Promoter

    Chicken Beta Actin

    Available Since

    June 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-EGFP/RFP-miR-E2-4int

    Plasmid

    #19820

    Depositor

    Zuoshang Xu

    Article

    Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

    Insert

    miRNA
    

    Use

    Cre/Lox and RNAi

    Expression

    Mammalian

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only