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We narrowed to 2,617 results for: pCas

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Showing: 2201 - 2220 of 2617 results

  1. pRhaB-8xHis-ABE8e-eVRQR (LLH551)

    Plasmid
    #242658
    Purpose
    pRhaB promoter expression plasmid for ABE8e-eVRQR with an N-terminal His8-tag
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pRhaB-8xHis-BPNLS-TadA8e-SpCas9(D10A)-eVRQR-BPNLS
    Use
    CRISPR
    Tags
    8x-His
    Expression
    Bacterial
    Mutation
    eVRQR mutations in SpCas9(S55R/D1135V/G1218R/R133…
    Promoter
    RhaB
    Available since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    242658-plasmid-map-sequence-id-491546

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  3. pSCBE3-NG

    Plasmid
    #218159
    Purpose
    This plasmid harbors the base editor SCBE3-NG along with an sgRNA cloning cassette, facilitating cytosine base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Publication
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-NG
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / L1111…
    Promoter
    rpsL promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218159-plasmid-map-sequence-id-433199

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  4. ABE-RA4.2

    Plasmid
    #208307
    Purpose
    Expresses ABE-RA4.2 in mammalian cells
    Depositor
    Weixin Tang
    Publication
    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TadA-RA4.2-SpCas9 D10A
    Expression
    Mammalian
    Mutation
    P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    208307-plasmid-map-sequence-id-410360

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  5. Polymerase expression - pCMV-B103 (LM2907)

    Plasmid
    #208968
    Purpose
    Unfused B103 DNA polymerase, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B103-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208968-plasmid-map-sequence-id-415815

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  6. BE3-hA3A-Y130F

    Plasmid
    #132945
    Purpose
    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.
    Depositor
    Hui Yang
    Publication
    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE3-hA3A(Y130F)
    Use
    CRISPR; Base editor
    Expression
    Mammalian
    Mutation
    BE3-hA3A(Y130F)
    Available since
    Nov. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    132945-plasmid-map-sequence-id-314826

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  8. sgALK(Chr. Inv.)

    Plasmid
    #85569
    Purpose
    Expresses sgRNA targeting human ALK between exon 20 and 21
    Depositor
    Luca Grumolato
    Publication
    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    anaplastic lymphoma receptor tyrosine kinase (ALK Human)
    Expression
    Mammalian
    Available since
    Feb. 8, 2017
    Availability
    Academic Institutions and Nonprofits only

  10. Polymerase expression - pCMV-Phi29(+exo) (LM2759)

    Plasmid
    #208964
    Purpose
    Unfused Phi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Phi29(+exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208964-plasmid-map-sequence-id-415810

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  11. AV13_pCAG.Cas9.gRNA.NT

    Plasmid
    #199260
    Purpose
    Expression construct encoding SpCas9 nuclease and a control non-targeting guide RNA
    Depositor
    Manuel Goncalves
    Publication
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    SpCas9 nuclease
    Non-targeting (NT) control gRNA
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG promoter and RNA polymerase III promoter for …
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    199260-plasmid-map-sequence-id-391374

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  12. PX459v2-ILK-gRNA 1-exon 1

    Plasmid
    #163320
    Purpose
    SpCas9 ILK gRNA 1 (G*CGGAGAACGACCTCAACCAG), targeting exon 1 within the N-terminal ankyrin repeat domain-1.
    Depositor
    Val Brunton
    Publication
    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ILK gRNA 1_Exon1
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only

  14. pPB_UCOE-CAG-2xMeCP2NLS-dCas9-HA-TagBFP-KOX1-rBGpA_chs4_EF1a-Blast-rBGpA

    Plasmid
    #254797
    Purpose
    Minimal construct for constituitve expression of dCas9-KOX1 with optimized nuclear localization in iPSC-derived neurons via transposase integration
    Depositor
    Ophir Shalem
    Publication
    Cajka et al bioRxiv [Preprint]. 2024 Oct 19:2024.10.
    Insert
    2xMeCP2NLS-dCas9-HA-TagBFP-KOX1
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    catalytically dead spCas9
    Promoter
    CAG
    Available since
    Sept. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    254797-plasmid-map-sequence-id-522131

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  16. pCMV-bpNLS-Stab-exin21-Bxb1(A315R)-Stab (CJT605)

    Plasmid
    #248211
    Purpose
    bpNLS-, stabilon-, and exin21-tagged Bxb1 (A315R) recombinase, expressed from a CMV promoter.
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586
    Insert
    bpNLS-Stab-exin21-Bxb1(A315R)-Stab
    Use
    CRISPR; In vitro transcription
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Bxb1(A315R)
    Promoter
    CMV and T7
    Available since
    April 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    248211-plasmid-map-sequence-id-506607

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  17. Recruited polymerase expression - pCMV-T7-EcKlenow-CC(N5) (LM2696)

    Plasmid
    #208969
    Purpose
    Recruited EcKlenow DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EcKlenow-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208969-plasmid-map-sequence-id-415816

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  18. PX459v2-ILK-gRNA 2-exon 8

    Plasmid
    #163321
    Purpose
    SpCas9 ILK gRNA 2 (G*ACATTGTAGAGGGATCCATA), targeting exon 8 within the C-terminal protein kinase domain.
    Depositor
    Val Brunton
    Publication
    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ILK gRNA 2_Exon8
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6F
    Available since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 2201 - 2220 of 2617 results