We narrowed to 2,296 results for: pET28

1. pMtb ATR

   Plasmid

   #138297

   Purpose

   Expression of Mtb ATR

   Depositor

   Ruma Banerjee

   Article

   Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

   Insert

   Corrinoid adenosyl transferase
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNIC28-Bsa4-ICL1

   Plasmid

   #105822

   Purpose

   Expression of Mycobacterium tuberculosis icl1 recombinant protein

   Depositor

   Ivanhoe Leung

   Article

   Bhusal et al Medchemcomm. 2017 Oct 17;8(11):2155-2163

   Insert

   icl1
   

   Use

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-28a-His6-SUMO-VAMP2

   Plasmid

   #135551

   Purpose

   Express mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   VAMP2
   

   Use

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. HpMscS

   Plasmid

   #78310

   Purpose

   H. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG Tag

   Depositor

   Douglas Rees

   Article

   Lai et al Protein Sci. 2013 Apr;22(4):502-9. doi:

   Insert

   Mechanosensitive channel of small conductance
   

   Use

   Tags

   6x His Tag and FLAG Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLyGo-Ec-6

   Plasmid

   #163132

   Purpose

   pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-28a-His6-SUMO-VAMP8

   Plasmid

   #135553

   Purpose

   Express mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   VAMP8
   

   Use

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. p28atDarpp

   Plasmid

   #108318

   Purpose

   Expresses tDarpp in E.coli

   Depositor

   Jamil Momand

   Article

   Momand et al FEBS Open Bio. 2017 Aug 29;7(9):1328-133

   Insert

   tDarpp (PPP1R1B Human)
   

   Use

   Tags

   6 Histadine

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZ004 (LseC2c2 locus)

   Plasmid

   #70170

   Purpose

   Expresses LseC2c2 locus with one spacer in array

   Depositor

   Feng Zhang

   Article

   Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

   Insert

   LseC2c2 locus with one spacer in array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

9. HOXB13 (human) HIS-tag pET

   Plasmid

   #8543

   Depositor

   Corey Largman

   Article

   Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

   Insert

   HOXB13 (HOXB13 Human)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   makes protein by Western and by transcription/tra…

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

10. Human USP30 (64-502, construct 13i, C77A, MG-31-28)

    Plasmid

    #110747

    Purpose

    Bacterial expression of human USP30 (construct 13i)

    Depositor

    David Komander

    Article

    Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

    Insert

    USP30 (USP30 Human)
    

    Use

    Tags

    N-His6-GST-3C

    Expression

    Bacterial

    Mutation

    C77A, F348D, M350S, I353E + insertion deletion: 6…

    Promoter

    T7

    Available since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-NEMOL329P

    Plasmid

    #11969

    Depositor

    Jon Ashwell

    Article

    Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

    Insert

    NEMOL329P (IKBKG Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Leucine329 to Proline

    Promoter

    Available since

    July 13, 2006

    Availability

    Academic Institutions and Nonprofits only

12. HOXD13 (chicken) HIS-tag pET

    Plasmid

    #8555

    Depositor

    Corey Largman

    Article

    Shen et al J Biol Chem 1997 Mar 28;272(13):8198-206

    Insert

    HOXD13 (HOXD13 )
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    contains 10 nt 5' to ATG start; makes prote…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

13. Dom34(265-386)

    Plasmid

    #19102

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Use

    Tags

    C tag and N tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

14. Dom34(265-377)

    Plasmid

    #19103

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Use

    Tags

    C tag and N tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pMIIS793

    Plasmid

    #238206

    Purpose

    Expresses TaTasR with an N-terminal Twin-Strep-SUMO tag. KanR

    Depositor

    Feng Zhang

    Article

    Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

    Insert

    TaTasR
    

    Use

    Tags

    TwinStrepII-bdSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. E547* FL USP7

    Plasmid

    #229001

    Purpose

    E547-mutant of human USP7

    Depositor

    Irina Bezsonova

    Insert

    USP7 1-546 (USP7 Human)
    

    Use

    Tags

    6xHis cleavable with thrombin

    Expression

    Bacterial

    Mutation

    E547 mutated to stop codon

    Promoter

    T7

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. PET-28a-YTH domain of YTHDC1

    Plasmid

    #228778

    Purpose

    Protein expression plasmid for YTH domain of YTHDC1 in E.Coli.

    Depositor

    Huiqing Zhou

    Article

    Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

    Insert

    YTH domain of YTHDC1 (YTHDC1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. SUMO CRYGS 16-177 Q16C

    Plasmid

    #217673

    Purpose

    N-terminally truncated human gamma S crystallin residues 16-177 with Q16C mutation for native chemical ligation

    Depositor

    Larry David

    Insert

    CRYGS (CRYGS Human)
    

    Use

    Tags

    6XHis and SUMO

    Expression

    Bacterial

    Mutation

    Glutamine 16 changed to Cysteine, and amino acids…

    Promoter

    T7

    Available since

    May 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. ppih.001.177.W133A

    Plasmid

    #137655

    Purpose

    Expresses N-terminal GST-(His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

    Depositor

    Tara Davis, Melissa Jurica

    Article

    Rajiv et al Biochem J. 2017 Oct 25;474(21):3689-3704

    Insert

    ppih (PPIH Human)
    

    Use

    Tags

    GST-(His)6-thrombin

    Expression

    Bacterial

    Mutation

    001-177-W133A

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-epELM-5

    Plasmid

    #179391

    Purpose

    Express a peptide (reside from 550 through 600) of human ELMSAN1 (Q6PJG2) in bacteria

    Depositor

    Shiro Iuchi

    Article

    Iuchi et al Commun Biol. 2021 Dec 15;4(1):1399. doi:

    Insert

    ELMSAN1 (MIDEAS Human)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac

    Available since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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