166,034 results

1. piggyBAC-U6-GFP-WPRE

   Plasmid

   #200030

   Purpose

   piggyBAC vector backbone with GFP for cloning DNA Ticker Tapes and U6 for pegRNAs

   Depositor

   Jay Shendure

   Type

   Empty backbone

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   U6, EF1a

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pExp-MBP

   Plasmid

   #112568

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   8xHis-MBP and Strep tag

   Expression

   Bacterial

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBMN mEGFP-OPTN

   Plasmid

   #188784

   Purpose

   For mammalian expression of OPTN tagged with mEGFP

   Depositor

   Michael Lazarou

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   OPTN (OPTN Human)
   

   Use

   Retroviral

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. EBFP2-N1

   Plasmid

   #54595

   Purpose

   Localization: N1 Cloning Vector, Excitation: 383, Emission: 448

   Depositor

   Michael Davidson

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   EBFP2

   Expression

   Mammalian

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pAcCas12nHS_RV6_empty

   Plasmid

   #203811

   Purpose

   Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.

   Depositor

   Quanjiang Ji

   Article

   Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

   Insert

   AcCas12nHS_RV6
   

   Use

   CRISPR

   Promoter

   CMV

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. L4440_BioBrick-SCR-sgRNA-B

   Plasmid

   #177812

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   Scrambled sgRNA targeting sequences
   

   Expression

   Bacterial

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Green Pegassos

   Plasmid

   #163114

   Purpose

   Expresses biosensors for pyruvate in mammalian cells

   Depositor

   Takashi Tsuboi

   Article

   Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

   Insert

   Green Pegassos
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW57.1-Tet-UPF1WT (pRKB264)

   Plasmid

   #99146

   Purpose

   Inducible lentiviral construct to express FLAG-tagged UPF1wt

   Depositor

   Robert Bradley

   Article

   Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

   Insert

   UPF1 (UPF1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   First 127 codons are optimized to reduce GC conte…

   Promoter

   pTight

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNCS-mRuby3

   Plasmid

   #74234

   Purpose

   Constitutive expression of mRuby3 in bacteria

   Depositor

   Michael Lin

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Insert

   mRuby3
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAVS1-CAG-hrGFP

    Plasmid

    #52344

    Purpose

    AAVS1 donor vector for constitutive gene expression

    Depositor

    Su-Chun Zhang

    Article

    Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

    Insert

    hrGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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