166,034 results

1. pUFlip-floxed2A-KalTA4-; HE1A:BFP -2

   Plasmid

   #180012

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-KalTA4; HE1A: BFP
   

   Use

   Synthetic Biology

   Available Since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-myc-Cdc42-wt

   Plasmid

   #12972

   Depositor

   Gary Bokoch

   Insert

   Cdc42 (CDC42 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a MDHP_YEAST

   Plasmid

   #204233

   Purpose

   Bacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MDHP_YEAST (MDH3 Budding Yeast)
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   The last 3 C-terminal residues (SKL) are the pero…

   Promoter

   Lac

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPRi

   Plasmid

   #199808

   Purpose

   vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

   Depositor

   Steve Salipante

   Article

   Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

   Inserts

   dCas9
   sgRNA with GFP cassette
   

   Use

   CRISPR

   Promoter

   P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3xFLAG-CMV14-GINIP

   Plasmid

   #204845

   Purpose

   Express 3x FLAG tagged-GINIP in mammalian cells

   Depositor

   Mikel Garcia-Marcos

   Article

   Park et al Mol Cell. 2023 Jul 20;83(14):2540-2558.e

   Insert

   PHF24 (KIAA1045 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Sept. 14, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. Hy_pMT Flag-UAP56 WT

   Plasmid

   #110125

   Purpose

   Expresses Flag-tagged human UAP56 (DDX39B)

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   UAP56 (DDX39B Human)
   

   Tags

   Flag

   Expression

   Insect

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGT-B2

   Plasmid

   #122580

   Purpose

   MAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. mPA-GFP-CD81-10

   Plasmid

   #57124

   Purpose

   Localization: Tetraspanin/Membrane, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Insert

   CD81 (CD81 Human)
   

   Tags

   mPA-GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pLKO.1-TetON-Puro-HIF1a 3809

   Plasmid

   #118705

   Purpose

   Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

   Depositor

   William Kaelin

   Article

   Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

   Insert

   shRNA against human HIF1a
   

   Use

   Lentiviral

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD_FLAG-GFP(Y39TAG)-6His

    Plasmid

    #223510

    Purpose

    Fluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39

    Depositor

    Edward Lemke

    Article

    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

    Insert

    GFP(Y39TAG)
    

    Tags

    6xHis and FLAG

    Expression

    Bacterial

    Mutation

    Y39TAG

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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