We narrowed to 4,223 results for: Pet2

1. pET22b-BirA-CPDSalI

   Plasmid

   #38258

   Depositor

   Aimee Shen, Matthew Bogyo

   Article

   Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

   Insert

   birA (birA E. coli)
   

   Use

   Tags

   6xHis and Vibrio cholerae MARTX toxin cysteine pr…

   Expression

   Bacterial

   Mutation

   cloned from pGEX4T1-BirA

   Promoter

   T7

   Available since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pET28a(His10-PD-1cyto)

   Plasmid

   #177896

   Purpose

   E coli expression of His10 tag fused to human PD-1 Intracellular Domain

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   PD-1 (aa 194-288 only) (PDCD1 Human)
   

   Use

   Tags

   10xHis-Thrombin cut site-T7 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-MSP1D1deltaH4/2H5

   Plasmid

   #71715

   Purpose

   Membrane scaffold protein (MSP), helix 4/2 + helix5 deletion

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Use

   Tags

   6xHis and TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21b-LOV-ipaA

   Plasmid

   #40236

   Depositor

   Brian Kuhlman

   Article

   Lungu et al Chem Biol. 2012 Apr 20;19(4):507-17.

   Insert

   LOV-ipaA
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Jα helix region hybridized (AsLOV2 aa 537-546/ipa…

   Promoter

   T7

   Available since

   Sept. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pET28a-mH6-Cas12i2

   Plasmid

   #120883

   Purpose

   Expresses E. coli codon optimized Cas12i2 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12i2
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lac

   Available since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET29b-ClbPpep-CHis

   Plasmid

   #48243

   Purpose

   First 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domain

   Depositor

   Emily Balskus

   Article

   Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

   Insert

   ClbP-pep
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   First 375 amino acids of the peptidase, which con…

   Promoter

   T7

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-baPrs-hdNadV

   Plasmid

   #91950

   Purpose

   pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi

   Depositor

   George Cătălin Marinescu

   Article

   Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

   Inserts

   Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
   Phosphoribosyl Pyrophosphate Synthetases
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed Leucine 135 to Isoleucine

   Promoter

   T7

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28_6xHis-PARP1-Y986H

   Plasmid

   #174801

   Purpose

   Bacterial expression of a PARP1 mutant that produces highly branched but short PAR chains overall

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-Y986H (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Y986H, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28_6xHis-PARP1-Y986S

   Plasmid

   #174802

   Purpose

   Bacterial expression of a PARP1 mutant that produces mainly short PAR oligomers

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-Y986S (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Y986H, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28_6xHis-PARP1-G972R

    Plasmid

    #174800

    Purpose

    Bacterial expression of a PARP1 mutant that produces less PAR oligomers overall

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-G972R (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    G972R, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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