We narrowed to 4,783 results for: Drosophila

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pWal10-roe-sfGFP

Plasmid

#240239

Purpose

Gateway destination plasmid to generate C-terminal sfGFP fusions under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Type

Empty backbone

Use

Gateway Cloning

Expression

Insect

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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Donor_YTHDF2_KO_Hygro

Plasmid

#186671

Purpose

Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Hygromycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

YTHDF2 KO Hygromycin (YTHDF2 Human)

Expression

Mammalian

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_YTHDF2_KO_Puro

Plasmid

#186670

Purpose

Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

YTHDF2 KO Puromycin (YTHDF2 Human)

Expression

Mammalian

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET21-His6dNLP

Plasmid

#105498

Purpose

Expression of N-terminally 6His tagged Drosophila nucleoplasmin-like protein

Depositor

James Kadonaga

Article

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

His6dNLP

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCaSpeR-act5cB-GAL4QF

Plasmid

#61309

Purpose

Expresses chimeric transactivator GAL4_DBD:QF2w_AD in Drosophila

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

GAL4_DBD::QF2w_AD

Expression

Insect

Mutation

Changed EK on C-terminus of QF2 to KKKK

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR_CG6867_nostop

Plasmid

#240238

Purpose

Gateway entry clone with CG6867 without stop codon

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

CG6867 (CG6867 Fly)

Use

Gateway shuttle vector

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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PRE-Hsp70BbCas9_1.2

Plasmid

#190796

Purpose

gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.

Depositor

Omar Akbari

Article

Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

Insert

gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP

Expression

Insect

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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puc19_N_tag

Plasmid

#186650

Purpose

Empty donor plasmid.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

N-terminal 3xFlag-3xHA Tag

Tags

3xFlag-3xHA

Expression

Insect

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET-SU(Z)12(624-900)-H6

Plasmid

#183772

Purpose

Express of SU(Z)12(624-900)-H6 in E. coli. Purified SU(Z)12(624-900)-H6 was used for raising polyclonal anti-SU(Z)12 antibody in guinea pigs.

Depositor

Peter Harte

Article

Tie et al Mol Cell Biol. 2003 May;23(9):3352-62. d

Insert

SU(Z)12 residues 624-900 (Su(z)12 Fly)

Tags

His tag at the C-terminus

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pExpreS2-1-CR-PA

Plasmid

#175445

Purpose

HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform

Depositor

Wyatt Yue

Article

Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

Type

Empty backbone

Tags

BiP Secretion Peptide and Thrombin-Protein A

Expression

Insect

Promoter

Fused Actin-HSP70

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pET24-ISWI-iCBD

Plasmid

#105499

Purpose

Expression of Drosophila ISWI C-terminally tagged with Combined Sce VMA intein and chitin binding domain (iCBD)

Depositor

James Kadonaga

Article

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

ISWI-iCBD

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-yki:V5-S168A

Plasmid

#85617

Purpose

Expresses V5 tagged mutant yki in Drosophila.

Depositor

Kenneth Irvine

Article

Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

Insert

yki-V5-S168A

Tags

V5

Expression

Insect

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pET-NDH6

Plasmid

#105503

Purpose

Expression of an N-terminally truncated form of Drosophila topoisomerase I with a C-terminal 6His tag

Depositor

James Kadonaga

Article

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

NDH6

Tags

6xHis

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-GMR-eya(shRNA)_EcoRImut

Plasmid

#240222

Purpose

pBS-GMR-eya(shRNA) vector with one of two EcoRI sites mutated (T->A)

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Type

Empty backbone

Expression

Insect

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_hhN-HA

Plasmid

#240225

Purpose

Gateway entry clone with N-terminal hedgehog (hhN) tagged with HA (contains stop codon)

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

hedgehog N-terminal fragment (hh Fly)

Use

Gateway shuttle vector

Tags

HA-spGFP11

Mutation

aa 1-257

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_spatzle-HA

Plasmid

#240227

Purpose

Gateway entry clone with spatzle tagged with HA (contains stop codon)

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

spatzle (spz Fly)

Use

Gateway shuttle vector

Tags

HA-spGFP11

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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GLE_sgRNA

Plasmid

#186658

Purpose

GLE sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Gle sgRNA Plasmid (CG14749 Fly)

Expression

Insect

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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piwi_sgRNA

Plasmid

#186653

Purpose

piwi sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Piwi sgRNA Plasmid (piwi Fly)

Expression

Insect

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Cas9_ANKRD1_sgRNA2

Plasmid

#186669

Purpose

Plasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

ANKRD1 KO sgRNA Plasmid (ANKRD1 Human)

Expression

Mammalian

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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Nbr_C_sgRNA2

Plasmid

#186664

Purpose

Nbr C-tag sgRNA2 plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Nbr sgRNA 2 Plasmid (Nbr Fly)

Expression

Insect

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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