We narrowed to 10,858 results for: Kars

1. pTPGI_dCas9_VP64

   Plasmid

   #49013

   Purpose

   encodes yeast-optimized dCas9_VP64 synthetic transcription factor

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   pTPGI (galactose+aTc inducible)

   Available since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEvoltac EcW-TGA-h9

   Plasmid

   #218765

   Purpose

   encodes EcWRS-h9 and EcW-tRNA-TGA

   Depositor

   Abhishek Chatterjee

   Article

   Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

   Insert

   EcWRS-h9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S8A, V144S, V146A

   Promoter

   tacI

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSSM110: pCMV-HA-NLS-SpuFz1-v2

   Plasmid

   #205277

   Purpose

   Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-v2

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1
   

   Use

   Tags

   HA, NLS(Makkerh)

   Expression

   Mammalian

   Mutation

   C310R/D487K/T513K

   Promoter

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSKI074

   Plasmid

   #11571

   Depositor

   Detlef Weigel

   Article

   Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

   Insert

   CaMV 35S enhancers
   

   Use

   Activation tagging

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUW-TetO-Sox9

   Plasmid

   #41080

   Depositor

   Rudolf Jaenisch

   Article

   Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

   Insert

   Sox9 (Sox9 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO + minimal CMV

   Available since

   Nov. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET22b-csgA

   Plasmid

   #89581

   Purpose

   Express csgA curli protein with GS linker and Histaq

   Depositor

   Urartu Seker

   Article

   Abdelwahab et al Langmuir. 2017 Apr 7. doi: 10.1021/acs.l

   Insert

   csgA (csgA )
   

   Use

   Tags

   3xGGSG linker - TEV site - 3xGGSG linker and 6xHi…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAB1620 hU6-BpiI-Cas13bt3-DR

   Plasmid

   #176315

   Purpose

   hU6-BpiI-Cas13bt3-DR (crRNA expression)

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

   Insert

   Cas13bt3 crRNA direct repeat
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO-RFP-shCntrl

   Plasmid

   #69040

   Purpose

   Lentiviral expression of control shRNA

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   control shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET22b-csgB

   Plasmid

   #89582

   Purpose

   Express csgB curli protein with GS linker and Histaq

   Depositor

   Urartu Seker

   Article

   Abdelwahab et al Langmuir. 2017 Apr 7. doi: 10.1021/acs.l

   Insert

   csgB (csgB )
   

   Use

   Tags

   3xGGSG linker - TEV site - 3xGGSG linker and 6xHi…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETM6-T7-VHH72

    Plasmid

    #202487

    Purpose

    Expresses the nanobody VHH72 with T7 induction

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody VHH72
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPomyces-AsCas12j-2

    Plasmid

    #191655

    Purpose

    All-in-one editing CRISPR-Cas construct for one-step genome editing of Streptomyces using AsCas12j-2

    Depositor

    Fong Tian Wong

    Article

    Tan et al bioRxiv 2021.10.28.465406

    Insert

    AsCas12j-2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsL(XC)-BbsI

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC-HBH

    Plasmid

    #88896

    Purpose

    Carries phiC31 integrase attB site flanked by cHS4 insulators.

    Depositor

    Michele Calos

    Article

    Zhao et al PLoS One. 2014 Apr 29;9(4):e96279. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pACλCI-β-flap (831-1057)

    Plasmid

    #53733

    Purpose

    Expression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Insert

    λCI-β-flap fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac UV5

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMJ548: pU6-SpuFz1_MS2-omegaRNA(75nt)-entry

    Plasmid

    #205269

    Purpose

    Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (BbsI guide entry site)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1 omegaRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMJ204: pU6-SpuFz1_omegaRNA(350nt)-entry

    Plasmid

    #205268

    Purpose

    Human expression vector for SpuFz1 omegaRNA 5' +275 nt extended scaffold (BbsI guide entry site)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1 omegaRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCHERRY3

    Plasmid

    #24659

    Depositor

    Tanya Parish

    Article

    Carroll et al PLoS One. 2010 . 5(3):e9823.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-FLAG- gate-pGK-HYG

    Plasmid

    #107397

    Purpose

    Gateway destination vector

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBMN HA

    Plasmid

    #188645

    Purpose

    This is the Lazarou lab's standard retrovirus vector backbone for expression of HA-tag fusions onto the N-terminus of cloned genes

    Depositor

    Michael Lazarou

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Flag-Rap1b

    Plasmid

    #118325

    Purpose

    Expression of bovine Rap1b

    Depositor

    Philip Stork

    Article

    Takahashi et al J Biol Chem. 2013 Sep 27;288(39):27712-2

    Insert

    Rap1b (RAP1B Bovine)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWALIUM-intron 3x UAS attB

    Plasmid

    #206360

    Purpose

    includes two standard UAS cassettes and one UAS cassette designed to co-express shRNAs/shRNA clusters and prodein coding sequences

    Depositor

    Erdem Bangi

    Article

    Teague et al PLoS Genet. 2023 Jun 2;19(6):e1010792. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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