We narrowed to 2,463 results for: T2A

1. pCRISPaint-mNeon-BlastR [M1G]

   Plasmid

   #171805

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and blasticidin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-T2A-BlastR
   

   Use

   Gene tagging

   Mutation

   BlastR: Changed Methionin 1 to Glycine.

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUW-tetO-hOKMminiSOX

   Plasmid

   #206383

   Purpose

   Expresses four genes (human OCT4, KLF4, c-MYC and mouse miniSOX) in mammalian cells, for lentivirus generation.

   Depositor

   Ralf Jauch

   Article

   Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

   Insert

   OCT4, KLF4, c-MYC, miniSOX (POU5F1, KLF4, MYC Human, Mouse)
   

   Use

   Lentiviral

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE2-TetOminiCMV-OKM

   Plasmid

   #136554

   Purpose

   Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Klf4, cMyc

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Oct4-T2A-Klf4-E2A-cMyc (Pou5f1, Myc, Klf4 Mouse)
   

   Use

   Lentiviral

   Expression

   Bacterial

   Promoter

   tetO-miniCMV (dox-inducible)

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. rTTA-gRNA-AAV

   Plasmid

   #213036

   Purpose

   This vector contains rTTA expressed under CMV promoter and gRNA expressed under U6 promoter

   Depositor

   Nadav Ahituv

   Article

   Nguyen et al Nat Biotechnol. 2025 Feb 4:10.1038/s4158

   Insert

   rTTA-T2A-mCherry
   

   Use

   AAV and CRISPR

   Promoter

   CMV

   Available Since

   Oct. 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB-TR-hCAII

   Plasmid

   #232480

   Purpose

   Tetracycline inducible PiggyBac vector expressing human CAII gene including flag-tag (DYKDDDDK) on the 3' end

   Depositor

   Lukas Trantirek

   Article

   Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

   Insert

   Human carbonic anhydrase II (CA2 Human)
   

   Use

   Transposon system

   Tags

   DYKDDDDK (FLAG-tag)

   Expression

   Mammalian

   Promoter

   CMV-TetO

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-K36M TAIL[1-44]-3AID-HA-2A-mCherryBSD

   Plasmid

   #225872

   Purpose

   Lentiviral expression of AID degron tagged H3.3(K36M) histone tail corresponding to amino acids 1-44 and mCherry fused BlasticidinR

   Depositor

   Lacra Bintu

   Article

   Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

   Insert

   H3.3(K36M) amino acids 1-44 (H3-3A, H3-3B Human, Synthetic)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3xAID HA and T2A-mCherry

   Expression

   Mammalian

   Mutation

   amino acids 1-44 from H3.3 K36M

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-GCN5

   Plasmid

   #65386

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   GCN5 (KAT2A Human)
   

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF2959

   Plasmid

   #144435

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   KAT2A (KAT2A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-KIF1B beta-FL

   Plasmid

   #79108

   Purpose

   Mammalian expression of GFP-KIF1B beta-FL

   Depositor

   Susanne Schlisio

   Article

   Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

   Insert

   GFP-KIF1B beta full length (KIF1B Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   exon 25 missing (natural splice varient)

   Promoter

   CMV

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

10. RFP-KIF1B beta-FL

    Plasmid

    #79111

    Purpose

    Mammalian expression of RFP-KIF1B beta-FL

    Depositor

    Susanne Schlisio

    Article

    Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

    Insert

    RFP-KIF1B beta full length (KIF1B Human)
    

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    exon 25 missing (natural splice varient)

    Promoter

    CMV

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pT22UbhaEng2aE4PCh(#271)

    Plasmid

    #184085

    Purpose

    cyclofen-inducible fish Eng2a (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    3HA-ZfEng2a-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    3xHA

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT22UbhaEng2bE4PCh(#272)

    Plasmid

    #184086

    Purpose

    cyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    3HA-ZfEng2b-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    3xHA

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT22gCfpUbm5En2E4PCh(#251)

    Plasmid

    #184083

    Purpose

    cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    5myc-chkEn2-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    5xMyc

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT22Ubm5Eng2bE4PCh(#284)

    Plasmid

    #184088

    Purpose

    cyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    5myc-ZfEng2b-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    5xMyc

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT22UbHEn2E4PCh(#262)

    Plasmid

    #184084

    Purpose

    cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    3HA-chkEn2-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    3xHA

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT22Ubm5Eng2aE4PCh(#283)

    Plasmid

    #184087

    Purpose

    cyclofen-inducible fish Eng2a (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    5myc-ZfEng2a-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    5xMyc

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-KIF1B beta-600-1400

    Plasmid

    #79109

    Purpose

    Mammalian expression of GFP-KIF1B beta-600-1400

    Depositor

    Susanne Schlisio

    Article

    Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

    Insert

    GFP-KIF1B beta (600-1400 amino acids) (KIF1B Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    exon 25 missing (natural splice varient)

    Promoter

    CMV

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pT2iApowriter2(#336)

    Plasmid

    #184104

    Purpose

    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    

    Use

    Zebrafish transient transgenesis

    Tags

    internal tag: 5myc

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    Zf-Ubi

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT22Apowriter2(#340)

    Plasmid

    #184105

    Purpose

    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    internal tag: 5myc

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    Zf-Ubi

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT22iuasvApoWtEos3N(#486)

    Plasmid

    #184107

    Purpose

    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-2nls-tEosFP-nls'-P2A-Casp9-ERT2
    

    Use

    Zebrafish transgenesis (blue eyes)

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    4xUAS

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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