Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 3,666 results for: antibody

Showing: 2221 - 2240 of 3666 results

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pT7RNAPNev-FSR22

Plasmid

#216338

Purpose

Arabinose-inducible PBad promoter driving expression of the evolved N-terminal portion (residues 1-179) of T7RNAP translationally fused to anti-SARS-CoV-2 RBD DARPin FSR22

Depositor

Mark Styczynski

Insert

T7RNAPN-FSR22 fusion

Expression

Bacterial

Available Since

Jan. 17, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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2XSmBiT-SARS-CoV-2 S1

Plasmid

#173430

Purpose

protein expression plasmid of 2XSmBiT-SARS-CoV-2 S1-IgG1 Fc

Depositor

David Nemazee

Article

Huang et al J Immunol. 2021 Jul 1;207(1):344-351. do

Insert

2XSmBiT SARS-CoV-2 S1-IgG1 Fc (S SARS-CoV-2)

Use

Luciferase

Tags

human IgG1 Fc

Expression

Mammalian

Mutation

furin cleavage site RRAR to GSAS

Promoter

CMV

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHybr_r2a>m2a(silent)-srt-his

Plasmid

#124807

Purpose

HDR template to knock-in FC silent mIgG2a isotype in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to FC silent producing cell lines.

Depositor

Ferenc Scheeren, Martijn Verdoes

Article

van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

Insert

Murine IgG2a (Fc silent)

Use

CRISPR; Hdr template

Tags

Histag (HHHHHH) and Sortag (LPETGG)

Expression

Bacterial and Mammalian

Mutation

L234A/L235A/N297A

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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PX459-gR2A_Hinge

Plasmid

#124811

Purpose

Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.

Depositor

Ferenc Scheeren, Martijn Verdoes

Article

van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

Insert

Cas9 – gRNA_R2A_hinge

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Bacterial and Mammalian

Mutation

R166H in PuroR (please see depositors comment bel…

Promoter

pUC

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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Trp1-Ala-display

Plasmid

#242868

Purpose

Expression of ECD of Trp-1-ala fused to display TMD.

Depositor

Ferenc Scheeren

Article

van der Plas-van Duijn et al Commun Biol. 2025 Nov 13;8(1):1561. doi:

Insert

Trp-1-ala (TYRP1 Human)

Use

Lentiviral

Tags

Display domain

Expression

Mammalian

Mutation

First amino acid after signal peptide mutated fro…

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-10c)

Plasmid

#241036

Purpose

Expresses SARS-CoV-2 spike (B.1-10c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-10c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T76R,K113T,K147N,S155R,N439R,T478K,E484V,S494R,K5…

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-9c)

Plasmid

#241053

Purpose

Expresses SARS-CoV-2 spike (B.1-9c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-9c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K113T,K147N,R158A,T478K,E484P,F490R,S494R,K529L,D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-9a)

Plasmid

#241051

Purpose

Expresses SARS-CoV-2 spike (B.1-9a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-9a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

H69G,Y248D,R403T,N439R,T478K,E484V,F490R,S494R,D6…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-9b)

Plasmid

#241052

Purpose

Expresses SARS-CoV-2 spike (B.1-9b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-9b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K113T,K147N,S155R,T478K,E484V,F490R,S494R,K529L,D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-8c)

Plasmid

#241049

Purpose

Expresses SARS-CoV-2 spike (B.1-8c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-8c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K113T,K147N,S155R,N439R,T478K,E484V,K529L,D614G,R…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-8d)

Plasmid

#241050

Purpose

Expresses SARS-CoV-2 spike (B.1-8d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-8d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

H69G,Y248D,R403T,N439R,T478K,E484V,F490R,S494R,D6…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-8a)

Plasmid

#241047

Purpose

Expresses SARS-CoV-2 spike (B.1-8a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-8a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K113T,R158A,K147N,N439R,T478K,S494R,F490R,D614G,R…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-8b)

Plasmid

#241048

Purpose

Expresses SARS-CoV-2 spike (B.1-8b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-8b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,S155R,R403T,N439R,T478K,E484V,K529L,D614G,R…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-5a)

Plasmid

#241046

Purpose

Expresses SARS-CoV-2 spike (B.1-5a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-5a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T76I,M153S,Y473F,N481T,Q493R,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-4b)

Plasmid

#241044

Purpose

Expresses SARS-CoV-2 spike (B.1-4b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,S494R,K529L,D614G,R683L

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-4c)

Plasmid

#241045

Purpose

Expresses SARS-CoV-2 spike (B.1-4c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

N439R,T478K,E484V,D614G,K529L

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-4a)

Plasmid

#241042

Purpose

Expresses SARS-CoV-2 spike (B.1-4a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,S494R,F490R,D614G,R683N

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-4d)

Plasmid

#241043

Purpose

Expresses SARS-CoV-2 spike (B.1-4d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T76I,M153S,Y473F,Q493R,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-10b)

Plasmid

#241035

Purpose

Expresses SARS-CoV-2 spike (B.1-10b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-10b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K113T,K147N,S155R,Y248D,T478K,E484V,F490R,S494R,K…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDMJ2-SARS-CoV-2-Spike (B.1-10d)

Plasmid

#241037

Purpose

Expresses SARS-CoV-2 spike (B.1-10d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-10d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

H69G,Y248D,R403T,N439R,T478K,E484V,F486E,F490R,S4…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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