We narrowed to 2,540 results for: organization Addgene

1. pMRX-IP-EGFP-PA-BAX(lyso, G108V)

   Plasmid

   #233345

   Purpose

   Stable expression of EGFP-LOV2(G528A, N538E)-BAX-TMEM106B in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX.

   Depositor

   Noboru Mizushima

   Article

   Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

   Inserts

   LOV2 domain of phototropin1
   BAX (BAX Human)
   TMEM106B (TMEM106B Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   BAX(3-171), N-terminus and C-terminus are deleted…

   Available Since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pF1-Hs Cdk1/cyclin B1

   Plasmid

   #177330

   Purpose

   Co-expression of the human active Cdk1/cyclin B1 kinase complex

   Depositor

   Andreas Boland

   Article

   Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

   Inserts

   Cyclin-dependent kinase 1 (CDK1 Human)
   G2/mitotic-specific cyclin-B1 (CCNB1 Human)
   

   Tags

   2 x Strep-tag and 8 x His

   Expression

   Insect

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH-EF1a-mCherry-EGFP-LC3B

   Plasmid

   #170446

   Purpose

   Lentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.

   Depositor

   Sang-Hun Lee

   Article

   Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

   Insert

   mCherry-GFP-LC3 (MAP1LC3B Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-EF1a-hDNAJC6-T2A-copGFP

   Plasmid

   #170443

   Purpose

   Lentiviral vector expressing human DNAJC6 with copGFP reporter gene, under control of EF1alpha promoter

   Depositor

   Sang-Hun Lee

   Article

   Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

   Insert

   human DNAJC6 (DNAJC6 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV x EF1a_EKAREN4-ires-puro

   Plasmid

   #167828

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available Since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB CAG SMARCA4-IRES-EGFP

   Plasmid

   #153948

   Purpose

   piggyBac transposon vector with CAG promoter expressing wild-type SMARCA4 (FLAG-tagged) and EGFP

   Depositor

   Luca Tiberi

   Article

   Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

   Insert

   SMARCA4 (SMARCA4 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-CMV-PEmax-IRES-ZeoR

   Plasmid

   #207353

   Purpose

   Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PEmax enzyme for prime editing.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   Prime editor max (PEmax) enzyme
   

   Use

   Lentiviral

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV x EF1a_EKAREN5-ires-puro

   Plasmid

   #167821

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN5
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. RKS-Fzd7/8 subtype NGS Wnt

   Plasmid

   #159628

   Purpose

   Express Fzd7/8 subtype NGS Wnt in mammalian cells

   Depositor

   Chris Garcia

   Article

   Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

   Insert

   Fzd7/8 subtype NGS Wnt
   

   Use

   Lentiviral

   Tags

   His tag

   Expression

   Mammalian

   Available Since

   Sept. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. P521_VSX2_p2A-h2b_mRuby3

    Plasmid

    #239104

    Purpose

    Used in CRISPR gene editing to Insert a p2A-h2b_mRuby3 sequence before the stop codon of the endogenous human VSX2 gene

    Depositor

    Karl Wahlin

    Article

    Jones et al Cells. 2022 Oct 28;11(21):3412. doi: 10.

    Insert

    VSX2 (VSX2 Human)
    

    Use

    CRISPR

    Tags

    -p2A-h2b-mRuby3

    Expression

    Mammalian

    Promoter

    Endogenous VSX2 promoter

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV x EF1a_EKAREN5-ires-blast

    Plasmid

    #167822

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-CMV-PE2-IRES-ZeoR

    Plasmid

    #207352

    Purpose

    Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PE2 enzyme for prime editing.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    Prime editor 2 (PE2) enzyme
    

    Use

    Lentiviral

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. RKS-Fzd7 subtype NGS Wnt

    Plasmid

    #159626

    Purpose

    Express Fzd7 subtype NGS Wnt in mammalian cells

    Depositor

    Chris Garcia

    Article

    Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

    Insert

    Fzd7 subtype NGS Wnt
    

    Use

    Lentiviral

    Tags

    His tag

    Expression

    Mammalian

    Available Since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-Fzd4 subtype NGS Wnt

    Plasmid

    #159625

    Purpose

    Express Fzd4 subtype NGS Wnt in mammalian cells

    Depositor

    Chris Garcia

    Article

    Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

    Insert

    Fzd4 subtype NGS Wnt
    

    Use

    Lentiviral

    Tags

    His tag

    Expression

    Mammalian

    Available Since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RKS-Fzd8 subtype NGS Wnt

    Plasmid

    #159627

    Purpose

    Express Fzd8 subtype NGS Wnt in mammalian cells

    Depositor

    Chris Garcia

    Article

    Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

    Insert

    Fzd8 subtype NGS Wnt
    

    Use

    Lentiviral

    Tags

    His tag

    Expression

    Mammalian

    Available Since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV x EF1a_EKAREN5(TA)-ires-puro

    Plasmid

    #167823

    Purpose

    control sensor for EKAREN5

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5(TA)
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Ad:2CMV_A2R2-N25Ctr

    Plasmid

    #122243

    Purpose

    Expresses a dominant negative Kash control, that lacks the actual Kash domain for LINC complex integration, in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone

    Depositor

    Corey Neu

    Article

    Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516

    Inserts

    C-terminal end of Nesprin-3 w/o Kash (Syne3 Mouse)
    Actinin alpha 2 (Actn2 Mouse)
    

    Use

    Adenoviral

    Tags

    Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…

    Expression

    Mammalian

    Mutation

    deleted Kash domain (GGCGAGGAGGAGCGCAGCTGCGCCCTGG…

    Promoter

    CMV

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. miniTol2 x EF1a_EKAREN5 + PGK-puro

    Plasmid

    #167817

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR

    Plasmid

    #207355

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 engineered prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    RNF2 + 5G>T tevopreq1-prime editing guide RNA (epegRNA)
    

    Use

    Lentiviral

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV x EF1a_EKAREN4-ires-blast

    Plasmid

    #167829

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge