We narrowed to 162,377 results for: addgene

1. AAV EGFP donor pDY0404

   Plasmid

   #219862

   Purpose

   AAV EGFP donor

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPB_EF1a_Gag-EIF4E-TM

   Plasmid

   #212658

   Purpose

   piggyBac vector to express an MLV Gag-EIF4E fusion protein (W56L, W102L, W166L mutations installed into EIF4E)

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   MLV Gag (UniProt ID: P03332) (gag MoMLV)
   

   Use

   Piggybac

   Tags

   EIF4E (W56L, W102L, W166L)

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPB_EF1a_Gag∆MA

   Plasmid

   #212654

   Purpose

   piggyBac vector to express MLV Gag with truncated MA domain

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   MLV Gag (UniProt ID: P03332) (gag MoMLV)
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of MA domain (amino acids 2-215)

   Promoter

   EF1a

   Available since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pZB604_pT7-sfGFP

   Plasmid

   #228510

   Purpose

   Bacterial Hybrid Reporter Plasmid (sfGFP), pT7-sfGFP

   Depositor

   Timothy Whitehead

   Article

   Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pZB641_pT7_Lysin-KU1

   Plasmid

   #228511

   Purpose

   T7 RNAP inducible Lysin KU1 Expression

   Depositor

   Timothy Whitehead

   Article

   Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

   Insert

   Lysin KU1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGFP-AUG-IRES-mCherry

   Plasmid

   #222109

   Purpose

   Start codon reporter (WT AUG)

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   GFP-IRES-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pST39-Mega'-deltaAll

   Plasmid

   #216878

   Purpose

   Derived from the plasmid for Mega GVs, pST39-pNL29 (Addgene #91696), but all gas vesicle proteins have been deleted.

   Depositor

   George Lu

   Article

   Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p8390 LentiCRISPRv2 Neo sgNT-2

   Plasmid

   #221650

   Purpose

   Expression of non-targeting control sgRNA

   Depositor

   Elizabeth White

   Article

   Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

   Insert

   non-targeting sgNT-2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MAC3-C-GFP-NLS

   Plasmid

   #222604

   Purpose

   Expression of MAC3-tagged GFP-NLS (C-terminal)

   Depositor

   Markku Varjosalo

   Article

   Liu et al Methods Mol Biol. 2023;2690:281-297. doi

   Insert

   EGFP and 5' nuclear localizaition sequence (NLS) (PPKTTRKVED)
   

   Use

   Tags

   MAC3

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 6, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. MAC3-N-GFP-NES

    Plasmid

    #222606

    Purpose

    Expression of MAC3-tagged GFP-NES (N-terminal)

    Depositor

    Markku Varjosalo

    Article

    Liu et al Methods Mol Biol. 2023;2690:281-297. doi

    Insert

    EGFP and 3' nuclear exclusion sequence (NES)
    

    Use

    Tags

    MAC3

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. MAC3-NTRK3

    Plasmid

    #222612

    Purpose

    Expression of MAC3-tagged NTRK3

    Depositor

    Markku Varjosalo

    Article

    Liu et al Methods Mol Biol. 2023;2690:281-297. doi

    Insert

    NTRK3 (NTRK3 Human)
    

    Use

    Tags

    MAC3

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. MAC3-N-GFP-NLS

    Plasmid

    #222607

    Purpose

    Expression of MAC3-tagged GFP-NLS (N-terminal)

    Depositor

    Markku Varjosalo

    Article

    Liu et al Methods Mol Biol. 2023;2690:281-297. doi

    Insert

    EGFP and 3' nuclear localizaition sequence (NLS)
    

    Use

    Tags

    MAC3

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. MAC3-C-GFP-NES

    Plasmid

    #222603

    Purpose

    Expression of MAC3-tagged GFP-NES (C-terminal)

    Depositor

    Markku Varjosalo

    Article

    Liu et al Methods Mol Biol. 2023;2690:281-297. doi

    Insert

    EGFP and 5' nuclear exclusion sequence (NES)
    

    Use

    Tags

    MAC3

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. MAC3-N-GFP-Myr

    Plasmid

    #222608

    Purpose

    Expression of MAC3-tagged GFP-Myr (N-terminal)

    Depositor

    Markku Varjosalo

    Article

    Liu et al Methods Mol Biol. 2023;2690:281-297. doi

    Insert

    EGFP and 3' myristoylation sequence (Myr)
    

    Use

    Tags

    MAC3

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. MAC3-C-GFP-Myr

    Plasmid

    #222605

    Purpose

    Expression of MAC3-tagged GFP-Myr (C-terminal)

    Depositor

    Markku Varjosalo

    Article

    Liu et al Methods Mol Biol. 2023;2690:281-297. doi

    Insert

    EGFP and 5' myristoylation sequence (Myr)
    

    Use

    Tags

    MAC3

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone

    Plasmid

    #219823

    Purpose

    EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)

    Depositor

    Philipp Maass

    Insert

    RfxCas13d
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSCL391

    Plasmid

    #219501

    Purpose

    Express Ade2 and Faa1 editrons from a single Gal7 promoter, separated by a Csy4 recognition sequence

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    Eco1 editing ncRNAs and Cas9 gRNAs to edit ADE2 and FAA1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL7

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LEGO-pDHS-AP1x6-GM55

    Plasmid

    #217416

    Purpose

    Luciferase expression vector with 6 AP-1 sites and the minimal GM-CSF promoter, and a chromatin priming element. Alias: LEGO-GM55-AP-1x6-pDHS(IL-3)

    Depositor

    Peter Cockerill

    Insert

    Luciferase, GFP
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    6 AP-1 sites, Human -55 to +28 minimal CSF2 promo…

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LEGO-pDHS-ITDE-AP1x6-DUSP5

    Plasmid

    #217419

    Purpose

    Luciferase expression vector with an oncogene-inducible enhancer, 6 AP-1 sites, the full length DUSP5 promoter, and a chromatin priming element. Alias: LEGO-DUSP5p-AP-1x6-ITDe

    Depositor

    Peter Cockerill

    Insert

    Luciferase, GFP
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human DUSP5 promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SHLD3 N-terminal sgRNA

    Plasmid

    #207094

    Purpose

    pX330 based plasmid for expression of Cas9 and the TTACTGCAGAATGACTACAG sgRNA to target the SHLD3 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    TTACTGCAGAATGACTACAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge