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We narrowed to 9,242 results for: ef1

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Showing: 3361 - 3390 of 9242 results

  1. lenti.Cas9.BFP.Blast

    Plasmid
    #196714
    Purpose
    WT SpCas9 with BSD-TagBFP for selection and sorting
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196714-plasmid-map-sequence-id-388043

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  2. lenti-RfxCas13d-Blast-BFP

    Plasmid
    #196726
    Purpose
    Sortable and selectable RfxCas13d.
    Depositor
    Bernhard Schmierer
    Insert
    RfxCas13d-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    SV40 NLS
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196726-plasmid-map-sequence-id-389737

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  3. pLV Tmx1-V5

    Plasmid
    #175138
    Purpose
    Lentiviral expression of V5-tagged mouse Tmx1
    Depositor
    Larry Gerace
    Insert
    Tmx1 (Tmx1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175138-plasmid-map-sequence-id-352052

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  4. pAAV_Efla_FLEX_APEX2-eGFPf

    Plasmid
    #171939
    Purpose
    Expresses a membrane targeted (farnesylation) APEX2-eGFP fusion protein
    Depositor
    David Berson
    Insert
    APEX2-eGFPf
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    Ef1a
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171939-plasmid-map-sequence-id-338504

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  5. BII-gR-PnTW

    Plasmid
    #133356
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Tags
    HA-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available Since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133356-plasmid-map-sequence-id-267393

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  6. pSBbi-Pur-AktAR2

    Plasmid
    #125195
    Purpose
    SB-transposon plasmid for stable expression of AktAR2 variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    AktAR2
    Use
    Transposon
    Tags
    6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125195-plasmid-map-sequence-id-243600

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  7. pLV Tmem9b-V5

    Plasmid
    #175152
    Purpose
    Lentiviral expression of mouse Tmem9b-V5
    Depositor
    Larry Gerace
    Insert
    Tmem9b (Tmem9b Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    175152-plasmid-map-sequence-id-352193

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  8. pLV Ndc1-V5

    Plasmid
    #175115
    Purpose
    Lentiviral expression of mouse Ndc1-V5
    Depositor
    Larry Gerace
    Insert
    Ndc1 (Ndc1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    175115-plasmid-map-sequence-id-351773

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  9. pSBbi-Puro_LPAR_AKAR-WT

    Plasmid
    #125206
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Eric Davis
    Insert
    LPAR_AKAR-WT
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125206-plasmid-map-sequence-id-243604

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  10. pEF-1a_RfxCas13d-HA-IRES-BFP-2A-BSD

    Plasmid
    #251220
    Purpose
    Lentiviral transfer plasmid for the generation of EF1a driven RfxCas13d expressing cells
    Depositor
    Niels Geijsen
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    251220-plasmid-map-sequence-id-504686

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  11. pEF-1a_dEGFP-Y66S-IRES-PuroR-WPRE

    Plasmid
    #251210
    Purpose
    Lentiviral transfer plasmid for the generation of EF1a driven dEGFP-Y66S expressing cells. Puromycin selection marker.
    Depositor
    Niels Geijsen
    Insert
    dEGFP-Y66S
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Y66S
    Promoter
    EF-1a
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    251210-plasmid-map-sequence-id-504677

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  12. lenti-dCas9-VP64-BFP

    Plasmid
    #196711
    Purpose
    CRISPR-activation. dCas9-VP64 with TagBFP for sorting
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-VP64-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available Since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    196711-plasmid-map-sequence-id-392561

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  13. pBHM2641

    Plasmid
    #229525
    Purpose
    Carries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    CRISPR
    Tags
    t2a-mNeonGreen-AID*
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    TEF1
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229525-plasmid-map-sequence-id-459550

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  14. pLV-Puro_hCOL4A4

    Plasmid
    #198099
    Purpose
    lentiviral expression of hCOL4A4 in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A4 (COL4A4 Human)
    Use
    Lentiviral
    Promoter
    EF1a
    Available Since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    198099-plasmid-map-sequence-id-389063

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  15. pPB-US-ECasE

    Plasmid
    #83961
    Purpose
    Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.
    Depositor
    Eleanor Chen
    Insert
    ERT2-spCas9-ERT2
    Expression
    Mammalian
    Mutation
    please see depositor comments below
    Promoter
    U6 (gRNA), EF1a (ERT2-Cas9-ERT2)
    Available Since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    83961-plasmid-map-sequence-id-214594

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  17. lenti-UCOE-dCas9-BFP-VP64

    Plasmid
    #196717
    Purpose
    CRISPR-activation. dCas9-VP64 with TagBFP for sorting.
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-TagBFP-VP64
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196717-plasmid-map-sequence-id-388038

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  18. Gal4-TEAD4

    Plasmid
    #24640
    Depositor
    Kunliang Guan
    Insert
    TEAD-4 (TEAD4 Human)
    Tags
    Gal4
    Expression
    Mammalian
    Available Since
    May 13, 2010
    Availability
    Academic Institutions and Nonprofits only

  19. pCAS9i_TRP

    Plasmid
    #141253
    Purpose
    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141253-plasmid-map-sequence-id-308890

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  20. pLV Tor1a-Myc

    Plasmid
    #175134
    Purpose
    Lentiviral expression of Myc-tagged mouse Tor1a
    Depositor
    Larry Gerace
    Insert
    Tor1a (Tor1a Mouse)
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175134-plasmid-map-sequence-id-352021

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  21. Cas9-ryt

    Plasmid
    #246021
    Purpose
    Expresses Cas9 under control of Hsp70 promoter and gRNAs targeting Lctra, yellow, and RFPex
    Depositor
    Max Scott
    Inserts
    Cas9
    RFPex
    Use
    CRISPR
    Tags
    NLS
    Available Since
    Jan. 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    246021-plasmid-map-sequence-id-498404

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  22. pLV V5-Senp2

    Plasmid
    #175118
    Purpose
    Lentiviral expression of V5-tagged mouse Senp2
    Depositor
    Larry Gerace
    Insert
    Senp2 (Senp2 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175118-plasmid-map-sequence-id-351807

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  23. pCAS9id_TRP

    Plasmid
    #141254
    Purpose
    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassettes with stuffer and Kpn restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141254-plasmid-map-sequence-id-308894

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  24. pSBBi-RP CCS1

    Plasmid
    #139162
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains coffee caffeine synthase 1 (CCS1); the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    caffeine synthase 1 [Coffea arabica]
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1α
    Availability
    Academic Institutions and Nonprofits only
    139162-plasmid-map-sequence-id-309352

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  25. pSBBi-GB CaXMT1

    Plasmid
    #139168
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains Coffea arabica xanthosine methyltransferase 1 (CaXMT1); the other side contains GFP and blasticidin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    7-methylxanthosine synthase 1 [Coffea arabica]
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1α
    Availability
    Academic Institutions and Nonprofits only
    139168-plasmid-map-sequence-id-309358

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  26. pLV-Hygro_hCOL4A3

    Plasmid
    #198098
    Purpose
    lentiviral expression of hCOL4A3 in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A3 (COL4A3 Human)
    Use
    Lentiviral
    Promoter
    EF1a
    Available Since
    April 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    198098-plasmid-map-sequence-id-467424

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  27. pLV-Puro_hCOL4A4-3FLAG

    Plasmid
    #198101
    Purpose
    lentiviral expression of hCOL4A4 in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A4 (COL4A4 Human)
    Use
    Lentiviral
    Tags
    3FLAG
    Promoter
    EF1a
    Available Since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    198101-plasmid-map-sequence-id-389065

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  29. pAAV-double floxed-eNpHR-EYFP-WPRE-pA

    Plasmid
    #20949
    Purpose
    Cre-activated AAV expression of halorhodopsin 2.0 (eNpHR) fused to EYFP for optogenetic inhibition
    Depositor
    Karl Deisseroth
    Has Service
    AAV9
    Insert
    halorhodopsin
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Promoter
    EF1alpha
    Available Since
    May 13, 2009
    Availability
    Academic Institutions and Nonprofits only
    20949-plasmid-map-sequence-id-200280

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  30. pSBBi-Pur GFP-TM

    Plasmid
    #139185
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains transmembrane-anchored GFP; the other side contains the puromycin resistance gene.
    Depositor
    Neal Devaraj
    Insert
    GFP-TM
    Use
    Transposon
    Expression
    Mammalian
    Mutation
    fused to membrane anchor
    Promoter
    EF1α
    Available Since
    March 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    139185-plasmid-map-sequence-id-309371

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Showing: 3361 - 3390 of 9242 results