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KBB382

Plasmid

#185085

Purpose

HA-tagged POM152 with glycosylation sites mutated

Depositor

Ken Belanger

Article

Belanger et al Genetics. 2005 Nov;171(3):935-47. doi: 1

Insert

POM152

Tags

HA

Expression

Yeast

Mutation

Pom152 N280A, N398A, N???A, N???A = Pom152Dglyc

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only
pTSB-FICAT_Payload-Esp3I

Plasmid

#179380

Purpose

Transposon vector prepared for Esp3I golden gate cloning of Transgene

Depositor

Marc Güell

Article

Pallares-Masmitja et al Nat Commun. 2021 Dec 3;12(1):7071. doi:

Type

Empty backbone

Expression

Mammalian

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAra-tns

Plasmid

#170632

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

VchTnsABC

Expression

Bacterial

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pBzCas13b-gRNA-2

Plasmid

#164859

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-2

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBzCas13b-gRNA-3

Plasmid

#164860

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-3

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLsCas13a-gRNA-3

Plasmid

#164867

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-3

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLsCas13a-gRNA-2

Plasmid

#164866

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-2

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2080

Plasmid

#155160

Purpose

Expresses Strep-MlaF_L266A)-(6xHis-TEV-MlaB)

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Mutation

MlaF_L266A

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2081

Plasmid

#155161

Purpose

Expresses (Strep-MlaF_Y261A_L265A_L266A)-(6xHis-TEV-MlaB)

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Mutation

MlaF(Y261A_L265A_L266A)

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1305

Plasmid

#155143

Purpose

Expresses MlaFB, His tag on MlaF N-term

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

(6xHis-TEV-MlaF)-MlaB

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1306

Plasmid

#155144

Purpose

Expresses MlaFB, His tag on MlaF C-term

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

(MlaF-6xHis)-MlaB

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1307

Plasmid

#155145

Purpose

Expresses MlaFB, His tag on MlaB N-term

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1308

Plasmid

#155146

Purpose

Expresses MlaFB, His tag on MlaB C-term

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(MlaB-6xHis)

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1833

Plasmid

#155149

Purpose

Expresses MlaF(1-250)-GCN4

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-250)-GCN4_Dimerization_Domain

Promoter

araBAD

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1957

Plasmid

#155151

Purpose

Expresses Strep-MlaF-(6xHis-TEV-MlaB)

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Mutation

added Streptavidin tag on MlaF N-terminus

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2074

Plasmid

#155155

Purpose

Expresses Strep-MlaF-(6xHis-TEV-MlaB_T52E)

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Mutation

MlaB_T52E

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2076

Plasmid

#155156

Purpose

Expresses (Strep-MlaF_K47A)-(6xHis-TEV-MlaB)

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Mutation

MlaF_K47A

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2077

Plasmid

#155157

Purpose

Expresses (Strep-MlaF_E170Q)-(6xHis-TEV-MlaB)

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Mutation

MlaF_E170Q

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2078

Plasmid

#155158

Purpose

Expresses (Strep-MlaF_Y261A)-(6xHis-TEV-MlaB)

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Mutation

MlaF_Y261A

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2079

Plasmid

#155159

Purpose

Expresses (Strep-MlaF_L265A)-(6xHis-TEV-MlaB)

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Mutation

MlaF_L265A

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1244

Plasmid

#155138

Purpose

Expresses MlaFED operon in E. coli

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaFE-[6xHis-2xQH-TEV-mlaD]-mlaC operon

Promoter

araBAD

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-pol1-N1

Plasmid

#160988

Purpose

Express a fragment of yeast pol1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

pol1

Tags

GST

Expression

Bacterial

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGST-pol1-N2-2A2

Plasmid

#160991

Purpose

Express a fragment of yeast pol1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

pol1

Tags

GST

Expression

Bacterial

Mutation

F58A/D62A

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-pol1-N2

Plasmid

#160989

Purpose

Express a fragment of yeast pol1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

pol1

Tags

GST

Expression

Bacterial

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGST-pol1-N1-2A2

Plasmid

#160990

Purpose

Express a fragment of yeast pol1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

pol1

Tags

GST

Expression

Bacterial

Mutation

F58A/D62A

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLPhygSaCas9

Plasmid

#123262

Purpose

Expresses Staphylococcus aureus Cas9 (SaCas9) in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

Insert

Staphylococcus aureus Cas9 (NEWENTRY )

Use

CRISPR; Leishmania

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSPneoSagRNAH

Plasmid

#123265

Purpose

Expresses gRNA of Staphylococcus aureus Cas9 (SaCas9) in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

Type

Empty backbone

Use

CRISPR; Leishmania expression

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pNeoSagRNAH

Plasmid

#123266

Purpose

Expresses gRNA of Staphylococcus aureus Cas9 (SaCas9) in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

Type

Empty backbone

Use

CRISPR; Leishmania expression

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
IgH P+ VA20

Plasmid

#127249

Purpose

IgH V-region

Depositor

Christine Milcarek

Article

Shell et al J Immunol. 2007 Dec 1;179(11):7663-73.

Insert

Promoter+V-region of IgH of A20 B cell line

Use

Other

Promoter

IgH

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pZF-AA-GCN

Plasmid

#127479

Purpose

coding sequence for synthetic zinc finger ZF-AA-GCN

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-AA fused with GCN4 leucine zipper

Use

Cell-free transcription–translation

Tags

GCN4 leucine zipper

Promoter

T7

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_07: Plas_tet_bD

Plasmid

#113600

Purpose

PE_07: Plas_tet_bD

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Plas_tet_bD

Expression

Bacterial

Promoter

Hybrid Promoter : Plas_tet_bD

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_6: Para_tet_bD

Plasmid

#113601

Purpose

PE_6: Para_tet_bD

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Para_tet_bD

Expression

Bacterial

Promoter

Hybrid Promoter : Para_tet_bD

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_5: Pxyl_Lac_bD

Plasmid

#113602

Purpose

PE_5: Pxyl_Lac_bD

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Pxyl_Lac_bD

Expression

Bacterial

Promoter

Hybrid Promoter : Pxyl_Lac_bD

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_4: Prhl_lac_bD

Plasmid

#113603

Purpose

PE_4: Prhl_lac_bD

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Prhl_lac_bD

Expression

Bacterial

Promoter

Hybrid Promoter : Prhl_lac_bD

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_2: Plas_lac_bD

Plasmid

#113605

Purpose

PE_2: Plas_lac_bD

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Plas_lac_bD

Expression

Bacterial

Promoter

Hybrid Promoter : Plas_lac_bD

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_1: Para_lac_bD

Plasmid

#113606

Purpose

PE_1: Para_lac_bD

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Para_lac_bD

Expression

Bacterial

Promoter

Hybrid Promoter : Para_lac_bD

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_15: Pxyl_lac_tet_bD

Plasmid

#113592

Purpose

PE_15: Pxyl_lac_tet_bD

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Pxyl_lac_tet_bD

Expression

Bacterial

Promoter

Hybrid Promoter : Pxyl_lac_tet_bD

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_14: Prhl_lac_tet_bD

Plasmid

#113593

Purpose

PE_14: Prhl_lac_tet_bD

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Prhl_lac_tet_bD

Expression

Bacterial

Promoter

Hybrid Promoter : Prhl_lac_tet_bD

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_12: Plas_lac_tet_bD

Plasmid

#113595

Purpose

PE_12: Plas_lac_tet_bD

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Plas_lac_tet_bD

Expression

Bacterial

Promoter

Hybrid Promoter : Plas_lac_tet_bD

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_11: Para_lac_tet_bD

Plasmid

#113596

Purpose

PE_11: Para_lac_tet_bD

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Para_lac_tet_bD

Expression

Bacterial

Promoter

Hybrid Promoter : Para_lac_tet_bD

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_10: Pxyl_tet_bD

Plasmid

#113597

Purpose

PE_10: Pxyl_tet_bD

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Pxyl_tet_bD

Expression

Bacterial

Promoter

Hybrid Promoter : Pxyl_tet_bD

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_09: Prhl_tet_bD

Plasmid

#113598

Purpose

PE_09: Prhl_tet_bD

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Prhl_tet_bD

Expression

Bacterial

Promoter

Hybrid Promoter : Prhl_tet_bD

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_08: Plux_tet_bD

Plasmid

#113599

Purpose

PE_08: Plux_tet_bD

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Plux_tet_bD

Expression

Bacterial

Promoter

Hybrid Promoter : Plux_tet_bD

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CH110: Plas_lac_bD

Plasmid

#104867

Purpose

CH110: Plas_lac_bD

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Plas_lac_bD

Expression

Bacterial

Promoter

Hybrid Promoter : Plas_lac_bD

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_16: Para_lac_eG

Plasmid

#104869

Purpose

PE_16: Para_lac_eG

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Plas_lac_eG

Expression

Bacterial

Promoter

Hybrid Promoter : Plas_lac_eG

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_17: Plas_lac_eG

Plasmid

#104870

Purpose

PE_17: Plas_lac_eG

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Plas_lac_eG

Expression

Bacterial

Promoter

Hybrid Promoter : Plas_lac_eG

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_18: Plux_lac_eG

Plasmid

#104871

Purpose

PE_18: Plux_lac_eG

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Plux_lac_eG

Expression

Bacterial

Promoter

Hybrid Promoter : Plux_lac_eG

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_19: Prhl_lac_eG

Plasmid

#104872

Purpose

PE_19: Prhl_lac_eG

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Prhl_lac_eG

Expression

Bacterial

Promoter

Hybrid Promoter : Prhl_lac_eG

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_20: Pxyl_lac_eG::sfYFP

Plasmid

#104881

Purpose

PE_20: Pxyl_lac_eG::sfYFP

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Pxyl_lac_eG

Expression

Bacterial

Promoter

Hybrid Promoter : Pxyl_lac_eG

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PE_21: Para_tet_eG::sfYFP

Plasmid

#104882

Purpose

PE_21: Para_tet_eG::sfYFP

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter: Para_tet_eG

Expression

Bacterial

Promoter

Hybrid Promoter : Para_tet_eG

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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